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Table of Content

    10 September 2020, Volume 59 Issue 17
    Reviews
    Research status and prospect of rice planting mechanization
    ZHANG Ni, ZHANG Guo-zhong
    2020, 59(17):  5-6.  doi:10.14088/j.cnki.issn0439-8114.2020.17.001
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    The research status and development trend of rice mechanized direct seeding and mechanized transplanting technology and equipment at home and abroad were summarized. According to China's rice planting conditions and planting system, the shortcomings of existing rice planting equipment were pointed out; rice transplanting was an important development direction of rice planting mechanization, and the research status of rice pot seedling transplanting was emphatically elaborated. Finally, the research direction and trend of rice planting mechanization technology were prospected.
    Research progress on the impact of urbanization and urban green space on urban birds
    HE Meng, YANG Miao-chen, PEI Ying-feng, YANG Yuan-jiao, LUO Xu, WANG Yu-xiao, MA Chang-le
    2020, 59(17):  11-15.  doi:10.14088/j.cnki.issn0439-8114.2020.17.002
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    Through reading the literature of “urban birds” from 2000 to 2019, three hot topics of urban ornithology research were summarized, namely, the impact of human interference on urban birds, the research on the relationship between urban green space, urbanization and urban bird community, and the impact of plant community composition on urban bird habitat. On this basis, the research status of urbanization and the impact of urban green space on urban birds was analyzed, and then the prospect of urban green space landscape design was put forward.
    Breeding & Cultivation
    Comparison of the effect of several drought-resistant agents on drought resistance and loss reduction in rice production
    HOU Lin, LUO Yan-ping, HOU Xiao-jing, LUO Dong-yu, WEI Qiao, LI Hong-ju, ZHOU Jia-hua, JIN De-ming, WANG Fu, WANG Xiong
    2020, 59(17):  16-18.  doi:10.14088/j.cnki.issn0439-8114.2020.17.003
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    In order to find suitable drought-resistant agents for rice(Oryza sativa L.) production, field experiments were conducted using three candidate drought-resistant agents, namely, FA Handilong, Hanliting, and abscisic acid. The results showed that in the case of drought, all the three drought resistant agents could increase the chlorophyll content of leaves and panicle length of rice. In addition, FA Handilong could increase the seed setting rate, and Hanliting could increase the number of grains per panicle. Both Hanliting and FA Handilong could significantly increase the grain yield(P<0.05). Rational application of these two drought-resistant agents in rice production may effectively relieve drought losses.
    Correlation between SPAD Value and nitrogen indicators in rice leaves at different growth stages
    CHEN Qiu-yu, HUANG Ying-hua, ZHANG Hua-jie, ZHOU Li-yan, ZHAO Li-jun, ZHANG Shan-xuan, XIA Li-sha, HONG Rui-xia, LI Yan-da, CHEN Qing-chun
    2020, 59(17):  19-24.  doi:10.14088/j.cnki.issn0439-8114.2020.17.004
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    In order to clarify the correlation between the average SPAD value of rice (Oryza sativa L. ) leaves at different growth stages and the nitrogen content, accumulation of aboveground parts under condition of different amount of nitrogen fertilizer, a randomized block cultivation experiment under different nitrogen fertilizer levels was conducted. The results showed that the change trend of SPAD values measured in the upper, middle and lower parts of rice leaves was similar to the average SPAD values, and the SPAD values gradually decreased from tillering stage to yellow maturity stage. During the rice growth cycle, the nitrogen content of rice leaves and stems decreased gradually from tillering stage to yellow maturity stage, and the difference of nitrogen content increased with the increase of nitrogen application. During the whole growth period of rice, the average SPAD value of leaves was positively correlated with nitrogen content of stems and leaves(P < 0.01). Therefore, the SPAD value of rice leaves can be used as a basis for regulating nitrogen application.
    Analysis of some quality characters of 14 wheat new lines
    CUI Wen-li, CHEN Rui-rui, WANG Hui, ZHENG Wen-yin
    2020, 59(17):  25-27.  doi:10.14088/j.cnki.issn0439-8114.2020.17.005
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    In order to summarize the general situation of some new wheat lines, and to provide reference for the production of high-quality special wheat and the extraction of materials with excellent quality traits, 14 wheat breeding high-generation stable lines were selected, and some quality traits were determined by near-infrared analyzer. The results showed that the sedimentation values of 14 wheat strains were all low, but some strains reached the standard of high quality strong gluten wheat, with higher bulk density, higher protein content and higher wet gluten content. By comparison, the selection of C1, C2 and C9 lines can be strengthened.
    Investigation on present production situation and suggestions on structural adjustment of summer maize in Xiangyang city
    LUO Zhen, TANG Hao-jun, ZHAO Yao, TAO Meng, LUO Zhen-mei
    2020, 59(17):  28-30.  doi:10.14088/j.cnki.issn0439-8114.2020.17.006
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    The present situation of summer maize (Zea mays L.) production in Xiangyang city was expounded from three aspectsvariety application, climate influence and processing application. The problems in planting structure adjustment of summer maize in the Xiangyang city were analyzed in combination with investigation, and the structural adjustment suggestions of “one stable and multiple adjustment” was put forward , that was, to develop disversified mode and improve planting level,and to steadily promote planting structure adjustment for production reference.
    Resource & Environment
    Climatic characteristics and circulation type of snowstorm in the northern Tibet in the past 31 years
    YU Yan-qun, XIONG Qiu-fen, Zuoma, De Ji Baizhen
    2020, 59(17):  31-37.  doi:10.14088/j.cnki.issn0439-8114.2020.17.007
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    Using the daily precipitation data, weather phenomena, and snow accumulation data of observatories in northern Tibet from 1988 to 2018, the climatic characteristics of snowstorms in the northern Tibet were analyzed by methods of meteorological diagnosis and wavelet analysis. The results showed that the spatial distribution of the number of snowstorm days, snow amount, maximum snow depth and annually average snow depth in the northern Tibet in the past 31 years generally showed a trend of more in the east than in the west, mainly concentrated in the east, with a significant regional difference. The number of the snowstorm and the amount of snow in the last 31 years showed a decreasing trend. The turning year of which was 2002, it was a snowy period before 2002, and it was a period of few snowfalls after 2002. After the 2000s, the number of snowstorms decreased at a rate of 2 stops every 10 years, and the amount of snowstorms decreased at a rate of 2.4 mm per 10 years. The season distribution of the snowstorm was bimodal, with the largest peaks occurring in April and May. The number of snowstorm days showed a periodic change of 3a, 5a, and 10a. Using NCEP to analyze the height field and wind field of the data, the circulation and impact system of snowstorm weather were summarized and concluded. The circulation pattern of the regional snowstorm in north Tibet could be divided into 4 types, namely, the Indian low-pressure type, the north ridge & south trough type, stepped trough type, and Balkhash lake low trough type. The southern branch trough, tropical depression, low vortex-shear and plateau trough were the main influence systems of the snowstorm in the northern Tibet.
    Study on the response relationship between the area change and climate of Dongting lake based on a long time series
    WANG Wei, SUI Bin, LIN Nan, CHEN Tai-long
    2020, 59(17):  38-42.  doi:10.14088/j.cnki.issn0439-8114.2020.17.008
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    Dongting lake is the largest reserve lake in the middle and lower reaches of the Yangtze river. It plays an important role in regulating the surrounding climate of the lake, and the climate also reacts on the lake. In order to study the response mechanism between the area change of Dongting lake and climate change, the long sequence data of water area of Dongting lake from 1989 to 2018 was used to analyze the spatio-temporal evolution characteristics of water area of Dongting lake, and the relationship between the change of water area and climate factors was studied. The results showed that the area of Dongting lake fluctuated significantly, with the largest monthly average area in July and the smallest in January. In recent 30 years, the water area of Dongting lake was in a general shrinking trend, and the annual maximum lake area and area change had a decreasing trend. According to the analysis of the lake area of nearly 30a with temperature and precipitation, the change of the water area of Dongting lake had a significant correlation with the surrounding climate conditions in the long-term evolution process, but the correlation was not significant in the short term. The change of lake area caused the temperature around the lake area to rise faster than the average rise rate of Hunan province and even the whole country.
    Effects of exogenous Se on the content of Cd and Se in rice grain under Cd pollution
    WU Juan, GUO Zhen
    2020, 59(17):  43-45.  doi:10.14088/j.cnki.issn0439-8114.2020.17.009
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    Exogenous Se fertilizer was applied during the growth of rice to explore the accumulation effect of exogenous Se on Cd in rice grain under heavy metal Cd pollution. The results showed that in the Cd pollution environment, exogenous Se could decrease the accumulation of Cd in rice grains.
    Study on fertilizer utilization ratio of cherry tomato under limited root cultivation with coconut-coir substrate
    TAN Hai-yan, ZHANG Zhen-rong, LIN Feng-ying, ZHANG Shao-ming, CHI Jing-di, TONG Jiang-yun
    2020, 59(17):  46-48.  doi:10.14088/j.cnki.issn0439-8114.2020.17.010
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    In order to implement substrates cultivation under root system control and improve the effect of fertilizer application, two different nutrient solution treatments were set up to study the utilization rate of nitrogen, phosphorus and potassium under coconut-coir cultivation of cherry tomato (Lycopersicon esculentum var. cerasiforme Alef.). The results showed that the utilization rate of nitrogen, phosphorus and potassium of two treatments were 58%,44%,64% and 59%,30%,67%, respectively. After the cultivation, the pH of coconut-coir substrate was decreased, the partial nutrients and the salt content were accumulated.
    Effect of different combination of organic and inorganic potassium on the yield,quality and soil regime of lettuce
    WANG Su-ping, DU Lei, HONG Juan, HUANG Xiang, ZHANG Gui-you, ZHANG Li-hong, LIAN Zhi-cheng, YE Li-xia, JIANG Li, CHEN Gang
    2020, 59(17):  49-53.  doi:10.14088/j.cnki.issn0439-8114.2020.17.011
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    In order to clarify the optimum proportion of organic and inorganic potassium fertilizer on lettuce (Lactucas sativa L. var. ramosa Hort.), the effects of different combination of organic and inorganic potassium on the yield, quality of lettuce, the content of soil microorganisms and soil enzyme activity in Wuhan were studied. The results showed that the application of potassium fertilizer promoted the increase of leaves, SPAD value, photosynthetic rate and transpiration rate, decreased the content of nitrate and increased soluble sugar in the leaves, and significantly increased the yield, potassium content and potassium accumulation of lettuce, and also increased the activities of urease, phosphatase and invertase in the rhizosphere of lettuce after harvest, and the number of bacteria and fungi that could be cultured in the soil. Under the condition of equal potassium application, the application of organic fertilizer could reduce the accumulation of nitrate in leaves, reduce the content of VC in leaves and increase the content of soluble sugar. The yield of lettuce, accumulation of potassium, apparent utilization rate and agronomic efficiency of potassium fertilizer with the ratio of organic and inorganic potassium of 3∶7 and 1∶1 were all higher than other potassium treatments, and the activity of urease and phosphatase in the soil with the ratio of 1∶1 was also higher.Analysis indicated that the application of organic and inorganic potash fertilizer could improve the yield, quality, fertilizer utilization rate and soil enzyme activity of lettuce, and the effect of organic and inorganic potash fertilizer was better than that of all chemical fertilizers. The ratio of 3∶7 or 1∶1 of organic and inorganic potash fertilizer had better effect.
    Analysis and ecological risk assessment on heavy metal contents of farmland soils in Jiangxia district, Wuhan city
    ZHANG Gui-you, WANG Su-ping, DU Lei, HUANG Xiang, JIANG Li, HONG Juan, LIAN Zhi-cheng, ZHANG Li-hong, YE Li-xia, CHEN Gang
    2020, 59(17):  54-57.  doi:10.14088/j.cnki.issn0439-8114.2020.17.012
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    A total of 149 soil samples were collected from farmland soils in Jiangxia district, Wuhan city. The contents of 8 heavy metals (Pb, Cd, Cr, Hg, As, Ni, Cu and Zn) were analyzed. The single factor pollution index and Nemerow synthetical pollution index were used to evaluate the heavy metals in farmland soils in this area based on the soil environmental quality risk management and control standard. The results showed that more than half of the farmland soils in Jiangxia distract were clean. The main pollution was Cd pollution, 40.27% of farmland soils were in the state of Cd pollution, about 1% of farmland soils were in the state of As, Ni and Zn pollution respectively, and no farmland soils were found to be contaminated by Cr, Cu, Hg and Pb.
    Rhizosphere soil microorganisms diversity of Cardamine violifolia O. E Schulz analyzed by Illumina MiSeq high-throughput sequencing system
    CHEN Fei-fei, CONG Xing, XIANG Ji-qian, LI Hong-ying, SUN Ju-zhi, XU Feng, XUE Hua
    2020, 59(17):  58-62.  doi:10.14088/j.cnki.issn0439-8114.2020.17.013
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    Taking nine soil samples growing Cardamine violifolia O. E Schulz from three area (Shuanghe town, Enshi city; Wufeng county, Yichang city, Hubei province and Shimen county, Changde city, Hunan province) as materials, the microbial community structure and diversity were analyzed by using Illumina MiSeq high-throughput sequencing system. The results showed that the microbial community composition of S1 soil sample form Shuanghe and S2 soil sample from Wufeng were similar, but the composition were quite different between S1 soil sample and S3 soil sample from Shimen, and were different between S2 and S3 soil sample. The diversity index and rarefaction curves analysis showed that in nine soil samples, the highest microbial diversity was No. S1.2 soil sample from Shuanghe and the lowest microbial diversity was No. S3.2 soil sample from Shimen. It indicated that the community diversity of Shuanghe soil sample was the highest and that of Shimen soil sample was the lowest. Proteobacteria was the dominant population in three area soil. The same phyla of the three area were Crenarchaeota, Acidobacteria, Actinobacteria, Bacteroidetes, Chlamydiae, Chloroflexi, Firmicutes, Gemmatimonadetes, Nitrospirae, OD1, Planctomycetes, Proteobacteria, Verrucomicrobia and WS3.
    Investigation and evaluation of ectomycorrhizal fungal resources in Xiangxidong forest park
    LI Na, DU Hui-juan, HE Fei
    2020, 59(17):  63-66.  doi:10.14088/j.cnki.issn0439-8114.2020.17.014
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    The Xiangxidong forest park is a region with special natural geographical conditions and excellent ecological environment, located in Ankang city, Shaanxi province. Yet, little information is available on ectomycorrhizal fungal (ECMF) resources associated with various trees in this region. In this study, the ECMF resources in Xiangxidong forest park were investigated by the method of field survey, specimen collection and laboratory identification. In total, 16 ECMF species of eight families were identified, belonging to 8 families, including 4 species of Boletaceae, 3 species of Cortinariaceae, 2 species of Russulaceae, 2 species of Tricholomataceae, 2 species of Cantharellaceae, 1 species of Strobilomycetaceae, 1 species of Lycoperdaceae and 1 species of Sclerodermataceae. Among them, ECMF was most widely distributed in coniferous and broad-leaved mixed forests, accounting for 75.00%, 37.50% of fungi were found in coniferous forests, and 62.50% of fungi were found in broad-leaved forest. In the view of economic value, the fungi included 9 species of edible fungi(Excl. those who have been poisoned and those who have been safe to eat it), 9 species of medicinal or anticarcinogenic fungi(including fungi that have medicinal and anti-cancer properties), and 7 species of poisonous fungi(including fungi that toxic to them). The ECMF resources in the Xiangxidong forest park are relatively abundant and have a good prospect for development and utilization.
    Plant Protection
    Study on the control effect of different pesticides on EmpoascaMatsumurascaonukii
    LIU Chao, PU De-qiang, HUANG Qiong, MAO Jian-hui, CHEN Yu, XIAO Ke-jun, LUO Xi
    2020, 59(17):  67-68.  doi:10.14088/j.cnki.issn0439-8114.2020.17.015
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    The field efficacy of three new agents of azadirachtin, eucalyptol and chlorothiazide on EmpoascaMatsumurascaonukii was studied. The results showed that the three agents had certain control effects on E. onukii. Among them, 0.3% emulsifiable azadirachtin 250 times liquid had lower control effect; 5% solution eucalyptus oil 300 times liquid had better control effect, the control effect on the 3th day after treatment was 66.92%, and the control effect on the 7th day after treatment was 57.72%. The 10% soluble powder chlorothiazolate 750 times solution had good control effect. During the test, each treatment did not have an adverse effect on the growth of tea trees, and it was safe and harmless. Azadirachtin and eucalyptol are biological pesticide, and are environmentally friendly and could be used interchangeably with other chemical agents to delay the development of drug resistance.
    Effects of food factors on the period and survival rate of the first and second stage larvae of Holotrichia parallela
    LIU Yan-tao, XI Guo-cheng, HU Tie-huan
    2020, 59(17):  69-71.  doi:10.14088/j.cnki.issn0439-8114.2020.17.016
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    Based on the indoor rearing methods of adult and larvae, the effects of different foods on the survival rate and the duration of the first and second instar larvae of Holotrichia parallela were determined. The results showed that the survival rates of the first and second instar larvae were significantly affected by different foods. The survival rates of the first and second instar larvae when they were fed with peanut roots were the highest, and it was lower when they were fed with cotton roots, peanut pieces and millet roots. When the first instar larvae was fed with potatoes, the development speed was fast and the concentration was high, while the larvae fed with cotton roots grew slowest and lasted the longest. The second instar larvae fed with potato, sweet potato and peanut roots developed faster, while the second instar larvae fed with cotton and millet roots developed slower and lasted longer. On the basis of this study, by adjusting the field layout and planting structure, the development duration of the larvae, the mortality rate, the population number and the harm to peanuts could be prolonged.
    Studies on the biological characteristics and phototaxis of the Hydrillodes repugnalis, a insect used for producing insect tea
    FENG Shi-xin, WEI Yu-guo, WEI Yu-qian
    2020, 59(17):  72-74.  doi:10.14088/j.cnki.issn0439-8114.2020.17.017
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    The purpose of this study is to study the biological characteristics and phototaxis of Hydrillodes repugnalis, and increase yield of insect tea. The source of insects were obtained by attracting wild adults and bred by close net cages, then the development process and life habits of adults, larvae and pupae was observed regularly. While, the effects of monochromatic light waves and frequency conversion light waves on the photochemotactic behavior of adult worms were studied by photoethological method. The results show that in the production area of Guangxi, there are 3 generations per year. Pupation is in mid-March and emergence is in late April in the next year. Sexual maturation and mating occurs 2~3 days after emergence, and oviposition occurs 5~7 days after emergence. The growth course of each generation is different. The first generation is about 64 days, the second is about 72 days, and the third generation is about 205 days. Larvae growth period is the longest and adult period is the shortest in all generations. Adults are lurking in shallow food gaps or dark places during the day, act from eight to nine o’clock of night, and mating at night. Most eggs lay on the leaf surface of food layer, and are scattered. The larvae are photophobic and pseudo-lethal. The larvae are active in a warm, humid environment, and are fed on uncaria, jujube and fragrant leaves. In the range of 340 to 420 nm, the adult’s tendency to light is high. Gender and wavelength have certain influence on the chemotactic rate. The female adults have high phototaxis rateat wavelength of 360 nm, 390 nm and 350 to 369 nm, and the male adults have high phototaxis rate at wavelength of 360 nm, 420 nm and 350 to 369 nm. The adults of Hydrillodes repugnalis have strong phototaxis and concentrate in the range of 350 to 369 nm.
    Horticulture & Local Products
    Effect of temperature and matrix on seed germination of Lupinus micranthus
    XU Lai-xian, AI Xun-ru, ZHANG Ying-tuan, ZHU Jiang
    2020, 59(17):  75-79.  doi:10.14088/j.cnki.issn0439-8114.2020.17.018
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    By measuring the germination rate, germination energy, growth and seedling growth process of Lupinus micranthus seeds under different temperature and matrix conditions, the effects of temperature and matrix on the germination of L. micranthus seeds were analyzed. The results showed that, temperature had a significant effect on seed germination and seedling growth, and was the most important environmental factor for L. Micranthus seed germination and seedling growth. 15 ℃ was the most conducive to obtain a higher germination rate and 25 ℃ was the most conducive to obtain high growth. The matrix had a very significant effect on the maximum growth of L. micranthus, and had a significant effect on the initial stage and germination energy, but had no significant effect on the germination rate, end stage, germination days and average daily growth. The most comprehensive advantage of the matrix was perlite, vermiculite was the most conducive to the growth of seedlings. Temperature and matrix had obvious interaction effects on germination rate, germination period index, germination energy and maximum growth, the optimal treatment of temperature and matrix was “15 ℃+local yellow heart soil” and “15 ℃+peat”.
    Effect of temperature on growth and development of Petunia hybrida
    BAI Yan-rong, WANG Jin-ying, JIANG Ya-lian
    2020, 59(17):  80-82.  doi:10.14088/j.cnki.issn0439-8114.2020.17.019
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    Taking Petunia hybrida double waterfall, single dream and single carpet as materials, the effects of temperature on the growth and development of P. hybrida were studied. The results showed that the growth and development of P. hybrida were influenced by temperature, the optimum temperature was 16~25 ℃, the plant height, crown width, lateral branches and the number of flowers increased the most at the constant temperature of 21~25 ℃, while the plants grew spindly and the number of flowers decreased when the temperature was 25~35 ℃. Temperature higher than 35 ℃ or lower than 15 ℃ inhibited the growth of P. hybrida, reduced disease resistance, large area of leaves wilting, or even dead.
    Effects of different shading treatments on flowering period and color quality of peony
    YUE Gao-feng, HAN Zhi-qiang, MA Jun-qiang, XU Xian-xin, YANG Wen-yue
    2020, 59(17):  83-86.  doi:10.14088/j.cnki.issn0439-8114.2020.17.020
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    Paeonia suffruticosa Andr var. papaveracea (Andr.) Kerner was used to study the effects of different shading treatments on the flowering period and color quality of P. suffruticosa. The results showed that the light conditions under different shading degrees had obvious effects on the flowering period and color quality of peony. The full flowering period under 40% and 60% shading was 3 and 6 days later than that under full sunlight and the number, diameter, fresh weight and flower rate of peony were the best. 20% shade significantly improved the ornamental value of red peony; Under 40% shading, except for purple, the ornamental value of white, pink, red and black lines decreased; The effect of 60% shading on the flower color of all tested color systems was very obvious, which seriously affected and reduced the ornamental value of flower color.
    Comparison of drought resistance of five kinds of Hainan winter crops
    YAN Shi-long, QIAN Kun, MENG Rui, AO Su
    2020, 59(17):  87-92.  doi:10.14088/j.cnki.issn0439-8114.2020.17.021
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    In this experiment, polyethylene glycol(PEG-6000) was used to simulate drought conditions, the effects of PEG-6000 on five species including Lycopersicon esculentum, Zea mays L., Brassica chinensis, Citrullus lanatus and Raphanus sativus seed germination and seedling growth were investigated. The results showed that the germination rate, germination potential, germination index, germination rate and average germination time of the five tested plants were obviously affected under high concentration PE-6000 treatment. The comprehensive evaluation of the subordinate function values showed that the order of drought resistance of the five tested plants was as followsZea mays L. > Brassica chinensis > Lycopersicon esculentum > Citrullus lanatus > Raphanus sativus.
    Animal Husbandry & Veterinary Medicine
    Effects of pioglitazone on oocytes maturation in vitro and parthenogenetic embryos development of pig
    LYU Ling-yan, WANG Yan-ling, PAN Xing-chen, ZHANG Jia-qing, WU Yong-shao, SUN Ru-yu, PAN Tian-biao, ZHANG Bing
    2020, 59(17):  93-96.  doi:10.14088/j.cnki.issn0439-8114.2020.17.022
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    Aiming to investigate the effects of pioglitazone(PIO) on oocytesmaturation in vitro and parthenogenetic embryos development of pig, the effect of different concentration PIO on oocytes maturation in vitro and parthenogenetic embryos developmental potency were compared. The results showed that the polar body excretion rate of 1.0 μmol/L PIO-treated group was significantly higher than that of 0.5 μmol/L PIO-treated group in maturation medium(P<0.05),was very significantly higher that of control an 1.5 μmol/L PIO-treated group(P<0.01). The mature oocytes cultured in different concentrations PIO were parthenogenetic activated, the experimental results showed that the cleavage rate of the 1.0 μmol/L PIO group was significantly higher than of the 0.5 μmol/L group in embryo culture medium(P<0.05), was very significantly higher than that of the control and 1.5 μmol/L PIO group (P<0.01), as for the blastocyst rate and the total number of cells, the group of the 1.0 μmol/L PIO-treated group was the highest among the groups (P<0.01). In conclusion, 1.0 μmol/L PIO in maturation medium could improve the maturation rate of porcine oocyte, also could significantly improve the blastocyst rate and the total number of cells of porcine parthenogenetic embryos.
    Aquatic Products
    Study on phages in control of common bacterial diseases in Macrobrachium nipponense and its application
    HANG Xiao-ying, YU Zhe, LYU Sun-jian, SHI Wei-da, YUAN Xue-mei, LIU Li
    2020, 59(17):  97-100.  doi:10.14088/j.cnki.issn0439-8114.2020.17.023
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    41 bacterial strains were isolated from water and shrimps of three Macrobrachium nipponense ponds, by 16S rDNA gene sequence analysis and colony morphology observation, the strains from water were preliminarily identified as Bacillus megaterium, Aeromonas caviae, Enterobacter roggenkampii,et al, the strains from shrimp were preliminarily identified as Aeromonas caviae, Citrobacter murliniae, Enterobacter roggenkampii, et al. The phage NTHP01 inhibition of bacteria was detected by drop injection methodthe results showed that, phage NTHP01 had good cracking effect on Bacillus, Aeromonas, Acinetobacter, Enterobacter, Citrobacter, Arthrobacter protophormiae, Orthomonad, Lactococcus garvieae, et al. Phage NTHP01 preparation was appliled in shrimp breeding pond, the preliminary results showed that, the yield of Macrobrachium nipponense increased by 338~412 kg/hm2, and the output value increased by 2.25 ten thousand ~3.00 ten thousand yuan/hm2.
    Study on the technique of pond cage culture of Siniperca chuatsi with compound feed
    LI Sheng-hua, HUANG Yong-tao, WANG Cong-dan, WU Fan, XIA Chen, ZHANG Cong-yi, HOU Hai-ying, CHEN Xia, XU Wei, ZHANG Liang-ming, SHI Yi-yuan, XIAO Zhi-hua, LI Xu-chun
    2020, 59(17):  101-106.  doi:10.14088/j.cnki.issn0439-8114.2020.17.024
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    In order to to study the technique of Siniperca chuatsi with compound feed, the artificial ecology was set up in three ponds, and the net cages were less than 10% of the total area of the pond. Fishes with different niche and feeding habits were controlled outside the cages to control the total biomass of the ponds; Siniperca chuatsi seedlings were placed in the cages. Feed a compound feed combined with a bait made with Chinese herbal medicine ingredients. The result showed that, no disease occurred during the breeding period. The water quality of the three test ponds was mild eutrophication for 52.4% of the time and moderate eutrophication for 47.6% of the time. The yield of commercial Siniperca chuatsi was 15 to 18 kg/m2, and the average pond production was 12 700 to 14 400 kg/hm2, breeding profit 194 400~249 900 yuan/hm2, commercial Siniperca chuatsi Besilewsky specifications were neat, catching and selling were convenient, and economic and ecological benefits were significant.
    Storage & Processing
    Study on extraction technology and content of coumarin from three varieties of Viburnum
    NIU Yin-yin, LIAO Teng-fei, WANG Li, SUN Min, XU Ping, LUO Xing-wu
    2020, 59(17):  107-111.  doi:10.14088/j.cnki.issn0439-8114.2020.17.025
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    The raw materials are Viburnum rhytidophyllum,Viburnum propinquum and Viburnum sargentii. The single factor experiment was carried out by selecting ethanol concentration, extraction time, extraction temperature and ratio of material to liquid as factors, absorbance as index, and then orthogonal test analysis was carried out at appropriate factor level and HPLC was used to carry out the experiment. The yield of coumarin from three varieties of Viburnum was compared by the optimized extraction process. The results showed that coumarin was found in all three kinds of Viburnum, Viburnum rhytidophyllum was the highest,Viburnum propinquum was the second, and Viburnum sargentii was the least. The optimum conditions of Viburnum rhytidophyllum and Viburnum propinquum were as followsThe concentration of ethanol was 55%, the extraction time was 60 min, the extraction temperature was 80 ℃, and the ratio of material to liquid was 1∶25. The optimum conditions of Viburnum sargentii were as followsThe concentration of ethanol was 55%, the extraction time was 80 min, the extraction temperature was 70 ℃, and the ratio of material to liquid was 1∶25. The content of coumarin was different in the extraction process of different varieties of Viburnum.
    Optimization of extraction technique of polyphenols from Hemsleya chinensis by response surface methodology
    ZHOU Zi-shun, DUAN Ke-zhao, QUAN Rui-lan, ZHANG Guang-hui, YANG Sheng-chao, ZHAO Yan
    2020, 59(17):  112-118.  doi:10.14088/j.cnki.issn0439-8114.2020.17.026
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    Taking Hemsleya chinensis as the experimental material, the Box-Behnken central composite design and response surface methodology were used to optimize the extraction conditions of polyphenols from Hemsleya chinensis. The extraction rate of polyphenols was used as index. Based on the results single factor experiments, a mathematical model of 4 factors and 3 levels center combinations was established with the Design-Expert 8.0.6 software on the extraction temperature, ethanol concentration, liquid to material ratio, extraction time. The results showed that the fitting degree of polyphenol extraction rate model was good and significant. The best process parameters were extraction time 59 min, ethanol concentration (V/V) 50 %, extraction temperature 74 ℃, and liquid to material ratio 16∶1(mL/g). Under these conditions, the extraction rate of polyphenols from H. chinensis was (1.479 6±0.004 1) mg/g.
    The low temperature extraction method of cellulose from corn straw
    SONG He-ming, JIA Hong-ge, HU Yu-jie, CHENG Hong-bo, ZHAO Xin-yi, WANG Qing-yu, XU Jing-yu, WANG Xue-feng
    2020, 59(17):  119-121.  doi:10.14088/j.cnki.issn0439-8114.2020.17.027
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    In this study, a new method of extracting cellulose from corn stalk at low temperature was explored. Cellulose was successfully extracted from corn straw by low temperature system. The cellulose samples were characterized by FTIR, TGA and XRD. The results showed that low temperature system was an environmental protection system, which could extract high purity cellulose from corn straw.
    Fuzzy mathematics comprehensive evaluation method for optimizing the formula of Hovenia dulcis beverage
    ZHU Xin-peng, REN Xing-xing, YUAN Zhi-ning, WEI Mei-juan, MING Jing, CHENG Zhu-lin, WANG Jin-meng
    2020, 59(17):  122-127.  doi:10.14088/j.cnki.issn0439-8114.2020.17.028
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    Using Hovenia dulcis concentrated juice as raw material through orthogonal test and based on single factor test, taking fuzzy mathematics comprehensive evaluation as index to optimize the formula. The results showed that,100 mL Hovenia dulcis beverage needed 20 mL Hovenia dulcis concentrated juice, the ratio of sucrose to acesulfame was 5.000 g∶0.020 g, the dosage of citric acid was 0.20 g, the ratio of lemon yellow to carmine was 0.100 mL∶0.050 mL, the ratio of xanthan gum to guar gum was 0.02g∶0.02g. The formula makes Hovenia dulcis beverage in harmony with sweet and sour, having delicate fragrance of Hovenia dulcis. It can be used in developing of Hovenia dulcis beverage.
    Preparation of calcium peroxide from kitchen waste egg shell and its application in fruit preservation
    YAN Zong-lan, YANG Fu-sheng, ZHAO Peng-ying, PAN Hong, BU Lu-xia
    2020, 59(17):  128-131.  doi:10.14088/j.cnki.issn0439-8114.2020.17.029
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    The eggshell of kitchen waste was pretreated to study the content of calcium peroxide produced by the reaction of eggshell with and without film. It was found that the content of calcium peroxide in the sample without membrane was 54.28%, and that in the sample with film was 50.97%. The preservation of bananas and strawberries with calcium peroxide was studied. With the passage of time, it was found that the higher the amount of calcium peroxide was added, the lower the weight loss rate of fruits was. The weight loss rate of samples without calcium peroxide preservative was the highest. The results showed that calcium peroxide could inhibit the water loss of bananas and strawberries, prolong the fresh-keeping period, and had a strong fresh-keeping effect.
    Application of CMC-Na in coating preservation of Chaenomeles sinensis
    XING Jie
    2020, 59(17):  132-136.  doi:10.14088/j.cnki.issn0439-8114.2020.17.030
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    In this study, sodium CMC was used as coating material to investigate the effects of coating solution on the storage and preservation of Chaenomeles sinensis, including the time-dependent change of mass loss, VC content, organic acid content, total sugar content, flavonoids content and total polyphenols content. In order to obtain the optimal treatment concentration of CMC-Na and extend the shelf-life of Chaenomeles sinensis, to provide references for the industrialization of Chaenomeles sinensis. The results showed that CMC-Na film could effectively reduce the mass loss of Chaenomeles sinensis during storage, inhibit the decrease of organic acid, total sugar, flavonoids, total polyphenols and the degrandation of VC. Among all the treatments, the massfraction of 1.0% in CMC-Na had the best preservation effect, which could effectively prolong the fresh-keeping period of Chaenomeles sinensis.
    Study on fresh-keeping condition of cut rose flowers for household use
    DENG Tian, HU Ting, YAN Li
    2020, 59(17):  137-141.  doi:10.14088/j.cnki.issn0439-8114.2020.17.031
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    To study the fresh-keeping effects of sprite, salt, vinegar, beer, vitamin C, detergent and citric acid on fresh cut rose flowers. The best fresh-keeping reagent, the best concentration, the best pretreatment temperature and the best length of cut rose were determined by single factor experiment. The best preservation combination was optimized by L9(34) orthogonal test with the best pretreatment temperature, the best concentration of reagent and the best length of flower branch as the factors and the changes of flower diameter, fresh weight and vase life as the indexes. The results showed that with the increase of vase time of cut rose flowers, the life of fresh-keeping liquid containing sprite was the longest, and it was 5 days longer than that of control group, and the time of reaching the maximum flower diameter was the longest, and the ornamental effect was the best. After low temperature pretreatment, the fresh-keeping effect of fresh-cut flowers was significantly improved, the life span of cut flowers was about 20 days. Different flower branch length had little effect on fresh cut flowers. The results showed that the best fresh-keeping reagent of cut rose was sprite, the best concentration was 2%, the best low temperature pretreatment was 3 ℃, the best length of flower was 30 cm. The optimum preservation conditions were low temperature pretreatment at 3 ℃, reagent(sprite) concentration at 2%, flower diameter length at 25 cm.
    Detection Analysis
    Determination of 13 pesticide residues in Wuniuzao tea by SPE-GC-MS/MS
    LEI Hai-yan
    2020, 59(17):  142-144.  doi:10.14088/j.cnki.issn0439-8114.2020.17.032
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    To simultaneously determine 13 pesticides in 50 samples of local tea Wuniuzao. The samples extracted by acetonitrile and dried by rotated, purified by Cleanert TPT column,then subjected to GC-MS/MS using internal standard method. The treated samples pesticide residues were not detected. The results showed that the Wuniuzao of the local mountain area were very low. This paper provides some basic theory for the development of local tea industry.
    Detection of biochemical components and quality analysis of ancient tea tree black tea of zhuquesi in Ziyang population species
    LUO Ting-ting, ZHANG Lin, HAN Hao-yu, CHEN Fang
    2020, 59(17):  145-149.  doi:10.14088/j.cnki.issn0439-8114.2020.17.033
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    The quality characteristics of the spring and autumn rosefinch black tea samples in 2018, were judged by compared with the main content of biochemical components and sensory quality evaluation. The results of sensory evaluation showed that spring rosefinch black tea was brown and bright, uniform and tight, sweet and lasting, and the soup color of red and bright. Autumn rosefinch black tea was slightly loose in shape, but still had the characteristics of sweet and mellow, and the soup color was bright and so on. The comprehensive scores showed that spring black tea belonged to the first grade second class tea, and autumn black tea belonged to the third grade first class high quality tea. The results of physical and chemical examination showed that the total ash and water extract content of spring and autumn black tea samples were in accordance with the national standard. The contents of tea polyphenols, caffeine, free amino acids and tea pigment were little different from those of black tea from other producing areas in China. The average content of trace element selenium is in the range of 0.038 1~0.263 0 mg/kg. The contents of heavy metals Pb,As,Hg and Cd were lower than the China national export quality standards of tea. Moreover, the 48 pesticide residues were not detected in spring and autumn tea samples. Therefore, it can be seen that the spring and autumn rosefinch black tea samples are according with the relevant national standards, and the whole level of the black tea reaches the pure natural organic high-quality black tea, whether it is in the sensory aspect or on the main contents and pesticide residues.
    Improving detection of malachite green residue in aquatic products by HPLC
    ZHU Zhi-qiang, TONG Wen-yu, ZENG Zhi, ZHANG Liang-ming, SHI Yi-fu
    2020, 59(17):  150-153.  doi:10.14088/j.cnki.issn0439-8114.2020.17.034
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    Malachite green, as a synthetic industrial dye of triphenylmethane, has been widely used in aquaculture as an insecticide and fungicide. GB/T20361—2006 for the detection of malachite green residues in aquatic products was found that had some disadvantages, such as tedious operation, poor recovery and precision during years of testing. The effects of different extraction times and purification times on the recovery rate, and improved the pretreatment and chromatographic conditions of GB/T20361—2006,reducing the number of extractions and purification operations reduces the flow rate of the chromatographic method. The improved method has a detection limit of 0.40 μg/kg, a recovery rate of 81.8%~100.8%, and a precision of 0.93%~8.32%, which all meet and exceed the national standard.
    HPLC analysis of sulforaphane in different kinds of leaf mustard (Brassica juncea Coss. var. foliosa Bailey) seeds
    LAN Mei, YANG Hong-li, HU Jing-feng, ZHANG Li-qin, XU Xue-zhong, HE Jiang-ming
    2020, 59(17):  154-157.  doi:10.14088/j.cnki.issn0439-8114.2020.17.035
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    In order to get the resources of leaf mustard with abundant sulforaphane,seeds of twenty cultivars were used to analyze and compare the contents of sulforaphaneby high performance liquid chromatography (HPLC). The results showed that the range ofvariatoin of sulforaphane was 11.79~226.72 mg/kg(DW) with significant differences among tested lines. The material (Tai le sijiqing) with highest content of sulforaphane,which is 226.72 mg/kg (DW),was about 19.2 times compared with the lowest one (Spring cobbage with round leaves) which is 11.79 mg/kg (DW). It provides an foundational material for further research on rule of variation and the mechanism of metabolism of sulforaphan.
    Determination of chromium in food by ionic liquid-graphite furnace atomic absorption spectrometry
    LING Yue-tao, PAN Rui-hua, WANG Yao-xue, WANG Fan, GUO Shao-fei, ZHANG Lin, ZHAO Li-yang, XING Jun
    2020, 59(17):  158-160.  doi:10.14088/j.cnki.issn0439-8114.2020.17.036
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    A method for the determination of chromium in food by ionic liquid -graphite furnace atomic absorption spectrometry was established. The sample was directly dissolved in 1-butyl-3-methylimidazolium chloride ([Bmim]-Cl) and added with 0.5 mL nitric acid. The pretreatment process could be completed within 5 minutes. The solution was directly detected by graphite furnace atomic absorption spectrometry. Chromium has a good linear relationship in the range of 2.58~20.64 μg/L, under the optimized spectral conditions, the detection limit of chromium is 0.04 mg/kg, the recovery of standard addition is 92.00%~101.65%, RSD is 4.11%~6.69%. The pretreatment method is green and safe, easy to operate and fast to process, and has a wide application prospect.
    Agricultural Engineering
    Design and experiment of the controller of shutter machine in solar greenhouse
    CUI Yu-xiang, ZHAO Hui-fang, ZHAO Liang, YANG Fang, YANG Rui, PEI Wei-feng
    2020, 59(17):  161-165.  doi:10.14088/j.cnki.issn0439-8114.2020.17.037
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    The daily operation of curtain rolling machine is one of the main work of winter heat preservation in chinese solar greenhouse. A controller based on the minimum system of STM8S003F3 microcontroller was designed. With timer and EEPROM, the control program was designed according to the timing principle, and the semi-automatic operation of the curtain rolling machine was realized. Through wireless communication, the controller had remote control operation function. The controller has the advantages of low cost, simple principle and reliable operation, and reduces the labor burden.
    Design of automatic weather station based on Aliyun Internet of things platform
    HUANG Jiao-yu, TANG Hai
    2020, 59(17):  166-169.  doi:10.14088/j.cnki.issn0439-8114.2020.17.038
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    In view of the difficulty of maintenance and high professional requirement of meteorological station used by governmentalmeteorological department, a design scheme of civil automatic weather station based on Aliyun Internet of things platform is proposed. The system design includes hardware and software. The hardware part includes the core circuit of AM3358 controller, Modbus sensor data acquisition circuit ,4G communication module and so on. The software part includes device side Link kit software development, cloud server side IoT Studio website application UI interface development, background data service development, message flow and so on. All functions of the six-element automatic weather station are implementedin the system, and some practical functions are added, such as sending the message to the mobile phone APP group when a specific weather parameter appears. The practical test shows that it has the advantages of simple maintenance and low cost, which is especially suitable for the observation of meteorological elements in non-meteorological departments such as agricultural production, station and port.
    Biological Engineering
    Method for preparing Escherichia coli electrotransformation competent cells by rapid desalting
    CAI Song, YANG Dong-cheng, TANG Mei, XIONG Yuan-yuan, WANG Jin-hua, WANG Yong-ze
    2020, 59(17):  170-174.  doi:10.14088/j.cnki.issn0439-8114.2020.17.039
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    A similar density gradient centrifuge method was adopted to establish an efficient and fast protocol for Escherichia coli electrotransformation. Angle rotor centrifuge was used to achieve the goal of rapid desalination of cells using 20% glycerol (V/V) /1.5% mannitol (m/V) solution as gradient medium. The ratio of bacteria suspension to gradient medium was 1∶5 (V/V) to form a density gradient, which led to effectively centrifugal desalination. After centrifugation the cells were resuspended in a mixture of 20% glycerol (V/V)/1.5% mannitol (m/V), sequentially electroporated at 15 kV/cm. This procedure was suitable for effective desalination. Electroporation at room temperature did not improve transformation efficiency. In this study, the electransformation efficiency can reach 4.2×109 CFU/μg DNA only by two centrifugation, which greatly simplifies the experimental procedure.
    Rapid identification of differential protein related to rice quality by iTRAQ
    ZHA Wen-jun, YOU Ai-qing
    2020, 59(17):  175-177.  doi:10.14088/j.cnki.issn0439-8114.2020.17.040
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    Using Ezhong No. 5 and Guichao No. 2 as materials, the iTRAQ technology was used to quickly identify rice quality differential proteins, so that candidate genes related to rice quality can be rapidly and effectively screened based on the differential proteins. The results showed that Ezhong No. 5 and Guichao No. 2 identified a total of 61 significantly different proteins in rice, including 21 up-regulated proteins and 40 down-regulated proteins. This technology can be widely used in the research and development of rice quality traits and combined with transgenic technology to cultivate high-quality rice varieties.
    Economy & Management
    Research on the dilemma and solution of the integrated development of urban and rural economy in China
    LI Ping-nyu, LIU Jun-xia
    2020, 59(17):  178-181.  doi:10.14088/j.cnki.issn0439-8114.2020.17.041
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    Starting from the imbalance of urban and rural economic development in China, the main difficulties faced by China's urban-rural economic integration development were analyzed. The policy of urban bias has far-reaching impact, the level of agricultural modernization needs to be fully improvedand the rural environmental problems need to be fully rectified. The realization path of the integration of urban and rural economy in China was put forward.
    Opportunities and challenges for agriculture, rural areas and farmers under the impact of COVID-19
    CAO Zhi-jie, YANG Xue
    2020, 59(17):  182-184.  doi:10.14088/j.cnki.issn0439-8114.2020.17.042
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    The huge challenges and development opportunities of China's agriculture, rural areas and farmers were analyzed under the impact of COVID-19. How to promote the common development of agriculture, rural areas and farmers with the cooperation of government, enterprises, social organizations and individuals was discussed.
    Research on the cultivation of new agricultural management subjects based on economic transformation
    WANG Ying
    2020, 59(17):  185-188.  doi:10.14088/j.cnki.issn0439-8114.2020.17.043
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    Under the background of economic transformation, cultivating new agricultural management entities is not only an inevitable requirement for economic structure adjustment and upgrading, but also an inherent need for agricultural industrial structure adjustment and upgrading. Based on grasping the logic of the new agricultural management subject cultivation system, the problems existing in the cultivation of new agricultural management subjects in the process of transformation was analyzed. The empirical analysis on the factors influencing the cultivation of new-type agricultural business entities was made. The corresponding countermeasures including improving the rural land circulation market, innovating financing methods, building agricultural socialized service system, establishing risk prevention mechanism and strengthening agricultural vocational and technical training were put forward, so as to better cultivate new agricultural business entities and promote economic transformation and upgrading.
    Study on the distribution of income from market circulation of rural collective business construction land
    BAI Shan-wen
    2020, 59(17):  189-192.  doi:10.14088/j.cnki.issn0439-8114.2020.17.044
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    At present, the entry of rural collective business construction land into the market has been approved by the Third Plenary Session of the 18th Central Committee of the Communist Party of China on“equal access to the market, same right and same price” between urban and rural unified construction land and state-owned land in 2013. At the same time, article 63 and article 34 of the “Land Management Law” also have relevant provisions on the entry of collective business construction land into the market. With the promotion of the reform of rural collective business construction land, there are more and more researches on the distribution of construction land transfer income. This study mainly analyzes the theoretical basis of rural collective business construction land, the risk of entering the market and the ideas and strategies of value-added income distribution in order to improve the reference value.
    Research on performance evaluation of rural sewage network governance based on BSC:Take Changyuan city,Henan province as an example
    ZHU Li-juan
    2020, 59(17):  193-198.  doi:10.14088/j.cnki.issn0439-8114.2020.17.045
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    The management and protection of rural water environment involves multi-stakeholders. The joint participation of multiple subjects are the key to improve the effectiveness of governance. Based on the analysis of the current status of rural sewage network treatment in Changyuan city, Henan province, the balanced score card is used as a performance evaluation tool, this study sets and evaluates the performance of rural sewage network governance in Changyuan city of Henan province from four dimensions of finance, customers, internal operations, learning and growth. The results show that the endogenous network governance model with villages as the main body, government guidance, and social resources support is more effective, which will help to open a new situation of rural sewage treatment.
    Institutional economics analysis on the three rights division of rural housing land:A case study of Changyuan city in Henan province
    XU Chun-lian
    2020, 59(17):  199-203.  doi:10.14088/j.cnki.issn0439-8114.2020.17.046
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    Taking “relative price change of land elements-benefit game-reform on the three rights division of rural housing land” as the main line, this study analyzes the institutional economics of the reform of the three rights division in Changyuan city, Henan province, by means of theoretical analysis and literature review. It is found that the change of relative price of land factors increases the external profit, which also becomes the practical demand of the reform of the separation of three rights division. The reform of the three rights division is conducive to the development of local economy, the improvement of farmers’ living environment, and the strength of collective economic organizations. It can also realize the internal internalization of external profits among farmers, governments and collective economic organizations through benefit game, so as to achieve a win-win situation. Therefore, we should respect the change trend of relative price of homestead elements, recognize the value attributes of homestead assets, construct a value-added revenue sharing mechanism agreed by all stakeholders, and manifest the institutional performance.
    Study on the change and efficiency of rural land resource utilization:A case on Anhui province
    ZHU Yuan-yuan
    2020, 59(17):  204-208.  doi:10.14088/j.cnki.issn0439-8114.2020.17.047
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    Agricultural production efficiency determines the level of rural regional economic development and the quality of people's life. The mode and structure of rural land use in Anhui province are changing constantly. By constructing multi-factor evaluation index, the land use efficiency was analyzed comprehensively by using data envelopment analysis. The results showed that the comprehensive efficiency of rural land use in Anhui province showed an increasing trend, and there were significant differences among different regions. Therefore,to improve the utilization efficiency of rural land use, we should strengthen the service function of the government, perfect the infrastructure construction, perfect the supporting system of land circulation and enhance the value of land resources.
    Study on the adaptability of the Three Gorges trans provincial migrants in the post immigration period:Based on the sample survey of resettlement sites in Tongling city, Anhui province
    NIE Qian-yu
    2020, 59(17):  209-213.  doi:10.14088/j.cnki.issn0439-8114.2020.17.048
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    The resettlement of the Three Gorges project is the largest project resettlement in China. By the end of 2009, nearly 200 000 rural migrants had been relocated in the Three Gorges Reservoir area. Since 2000, the first group of immigrants to cross the province had been living there for nearly 20 years. The social adaptation of the immigrants in Tongling city, Anhui province is related to the survival and development of the immigrants in the Three Gorges, and also has an important impact on the community management and economic development of the immigrations. This study analyzed the adaptability of the Three Gorges immigrants in Tongling city, Anhui province by mean of literature method, interview method and field observation method, and studied the social adaptability of immigrants from the aspects of economy, politics, culture and social environment. It was found that there were some problems such as unsatisfied compensation, poor housing quality, petition for rights protection and disturbance and so on. In the post immigration period, we need to pay attention to the problems of employment, prosperity and ecological environment construction of immigrants.
    Research on the supply of rural public services in county areas from the perspective of collaborative governance
    XIANG Jia
    2020, 59(17):  214-217.  doi:10.14088/j.cnki.issn0439-8114.2020.17.049
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    Based on the theory of collaborative governance, focusing on county rural areas, the basic elements of county rural public service supply, namely, the four collaborative subjects of farmers' demand, government, market and other multiple subjects of society were extracted. The collaborative structure was analyzed from the strategic level, structural level and operational level; the county rural public service supply system was expounded with the collaborative process of establishing trust relationship, reaching ideological consensus, effective participation and collaborative ability. On this basis, the optimization path of public service supply from the perspective of collaborative governance in terms of concept updating and strategy selection was put forward.