HUBEI AGRICULTURAL SCIENCES

Research Progress in Volatile Substances of Congou Black Tea

GUI An-hui, GAO Shi-wei, YE Fei, GONG Zi-ming, WANG Xue-ping, ZHENG Peng-cheng, TENG Jing, WANG Sheng-peng, ZHENG Lin, LIU Pan-pan

2018, 57(18): 5-9. doi:10.14088/j.cnki.issn0439-8114.2018.18.001

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Congou black tea is a representative category of black tea in China.It is famous for its unique sweet,fragrant honey fragrance and fruit aroma. Volatile substances have always been the key research direction of congou black tea. In this paper,the volatile substances characteristics, extraction methods, processing process（withering, rolling, fermenting, drying） and the formation mechanism of the congou black tea were reviewed in four aspects,and the trend of the volatile substances in the congou black tea was prospected.

The Status and Problems of Wasps Artificial Breeding

HE Qiu-ju, YI Chuan-hui, ZHANG Zheng-wang, REN Xi-peng, LIN Zhong-yang

2018, 57(18): 10-13. doi:10.14088/j.cnki.issn0439-8114.2018.18.002

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Wasps are kinds of important insects resource, which have high value of edible,medicinal,health for people,and nature enemy for pests. The basic research,present situation and existing problems of artificial breeding of wasps were summarized in this paper. And at the same time,suggestions were put forward to strengthen the basic research to promote the rapid development of industry of wasps.

Effect of Density and Plants per Hill on Yield Characters of Millet Variety Jingu 29

HANG Li, ZHANG Xia, ZHANG Rui, LI Rui

2018, 57(18): 14-17. doi:10.14088/j.cnki.issn0439-8114.2018.18.003

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Experiment was conducted with millet variety Jingu 29 as materials to study the effects of density and plants per hill on the reproductive traits and economic traits. The optimal planting density and plants per hill of millet in the shenmu region were analyzed to provide theoretical basis for the high yield and high efficiency cultivation,and the mechanized seeding by small machines. Results showed that different densities and plants per hill had a great impact on the agronomic characters and yield of Jingu 29. With the increase of density,the plant height,ear length,ear diameter and grain weight per ear decreased,and the yield was influenced. The highest yield of Jingu 29 was 5 050.25 kg/hm² when the density was 180 000 plant hm² with 2 seedlings per hill on dry land.

Effects of Different Film Color and Fertilizer on Potato Growth,Yield and Quality

HE Qiang-qiang, HE Wen-shou, HE Jin-qin, WEI Chun-yu, WEI Zi-qi

2018, 57(18): 18-22. doi:10.14088/j.cnki.issn0439-8114.2018.18.004

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Potato variety Qingshu 9 was used to study the effects of different film colors and fertilizer types on potato growth, yield and quality in the southern mountainous area of Ningxia. Two factor random block design with nine treatments combining 3 film colors （no film, white film and black film） and 3 fertilizers （no fertilizer,formula fertilizer and controlled-release fertilizer） was used. Results showed that treatment of formula fertilizer with white film cover had fast growth, short growth period and the highest yield of 32 515 kg/hm². Treatment of controlled-release fertilizer with black film cover showed the highest potato starch content of 19.81 g/100 g. Treatment of formula fertilizer with black film cover got the highest potato vitamin C content of 23.41 mg/100 g. These results could provide certain reference for the potato industry in the southern mountainous area of Ningxia.

Improvement of Rice Blast Resistance in Red Rice by Pyramiding of Pi1 and Pi2 through Molecular Marker-Assisted Selection

SUN Fu, TANG Mei, HE Cong, LU Hong-cong

2018, 57(18): 23-27. doi:10.14088/j.cnki.issn0439-8114.2018.18.005

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Red rice is an extremely precious characteristic rice resource. Blast is one of the most serious rice diseases worldwide,which usually leads to a sharp decline in rice yield and even results in no yield. Breeding blast-resistant varieties is one of the most effective and economical ways to control the disease. In this study,BL122 containing two blast resistance genes Pi1 and Pi2 was used as gene donor to be crossed and then backcrossed with red rice variety Guihong 1. The improved materials of high resistance to rice blast with two resistance genes were obtained. The resistance identification of rice blast in seedling stage and mature stage of BC₃F₃ was carried out. The results showed that the resistance of target strains with two resistance genes was all above the resistance level,and some strains reached high resistance level. The resistance of these strains to rice blast was significantly higher than that of the recurrent parent. Statistical analysis showed that,compared with the control,the effective panicle and theoretical yield of BC₃F₃ were significantly increased, while there was no significant difference in other agronomic traits. The improved materials obtained through molecular marker-assisted selection is a new breeding resource for the breeding of red rice.

Regional Experiment of Spring-sowed Maize Variety in the Hilly Plain

ZHANG Jun-shou, HUANG Jie, ZHOU Lin

2018, 57(18): 28-30. doi:10.14088/j.cnki.issn0439-8114.2018.18.006

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In 2017,the regional experiment of spring-sowed maize（Zea may L.） group in the hilly plain was carried out at Dangyang city in Hubei province. Six varieties including dunyu 339,TL757,Tianhan 10,NKY1501,and Jindan485,were involved in the experiment,mean while,taking Yidan 629 as control. The results showed that,the yield of Jindan485 and Dunyu 339 were more than control,and were 8 362.5 kg/hm² and 8 053.5 kg/hm²,increased by 9.98% and 5.92% compared with control,respectively.

Cultivation and Management of Spring-sowing Maize in Jianghan Plain

OUYANG Dun-jun, WEN Chao, ZHANG Yi-zhuo, GENG Bo

2018, 57(18): 31-33. doi:10.14088/j.cnki.issn0439-8114.2018.18.007

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The climate characteristics, suitable planting density,occurrence characteristics and relevant measures for the prevention and control of diseases,pests and weeds of spring-sowing maize at growth and development period in Jianghan plain were analyzed,and a simplified cultivation technique for planting spring-sowing maize of Jianghan plain was discussed,some cultivation practices such as reducing inputs of manpower and material resources,saving cost and increasing efficiency,to increase farmers' profits from spring-sowing planting.

Study on the Relationship between Air Quality and Meteorological Factors and Its Prediction Method in Chuzhou

XIONG Shi-wei, WANG Man-li, DENG Han-qing, DENG Xue-liang, QIU Yang-yang, HU Shan-shan

2018, 57(18): 34-38. doi:10.14088/j.cnki.issn0439-8114.2018.18.008

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Based on the daily air quality data and meteorological data of January 2015 to May 2017 in Chuzhou,the relationship between air quality and meteorological factors were analyzed,and an air quality prediction model based on meteorological factors was established,the results showed that air pollutants were dominated by PM2.5,mainly in winter and spring,followed by O₃,mainly in summer. When Chuzhou had a moderate pollution in the region,it played an important role in the transportation of pollution from the northwest. Wind speed and precipitation were negatively correlated with AQI and all kinds of pollutants,there was a significant negative correlation between temperature and PM10,PM2.5,NO₂,CO. Temperature was positively correlated O₃. Sunshine hours and relative humidity were significantly positively correlated with PM10 and PM2.5 concentrations. The prediction model had good prediction effect on AQI,PM10,PM2.5 and O₃ which could meet the daily business requirements,but had relatively poor prediction effect on NO₂,the standardized mean error（NME） was over 30%,and its concentration was overestimated.

Study on the Purification of A Biogas Slurry of Chlorella pyrenoidosa

TIAN Chen-xue, LIU Ming, HUANG Kai-yao, JIANG Si-wen, GAO Qi-shuang, MIAO Wen

2018, 57(18): 39-43. doi:10.14088/j.cnki.issn0439-8114.2018.18.009

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In order to treat biogas slurry more economically and effectively, microalgae was used to purify biogas slurry. The aim was to fully detect the changes of biogas slurry at different times and different indexes during purifying 10% diluted biogas slurry by a Chlorella pyrenoidosa for 18 days. The results showed that the algae chlorella pyrenoidosa could survive in the biogas slurry after disinfection and had a good growth status whose highest OD_{680 nm} could reach 1.206,highest relative growth rate could reach 0.208 0. The removal rates of total nitrogen,total phosphorus and ammonium nitrogen in biogas slurry were 61.89%,99.72% and 96.79%,respectively; The contents of NO₃^--N and NO₂^--N increased a little; And COD content showed a fluctuation curve. The feasibility of purifying the biogas slurry by C. pyrenoidosa was proved.

Analysis of Appropriate Dosage of Boron Fertilizer for Oilseed in Macheng Region

CHANG Hai-bing, HU Hai-zhen, XIAO Qi-sheng, LU He-ping

2018, 57(18): 44-46. doi:10.14088/j.cnki.issn0439-8114.2018.18.010

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Based on the test of different boron fertilizer usage in Macheng showed that 9.0 kg/hm² borax（boron content 12%） processing for oilseed rape growth and yield increase effect was best. At the same time,borax and oilseeds simulcasting was not affect the application of rapeseed and could improve the utilization rate of boron fertilizer,which was a more effective boron fertilizer application technique.

Purification of Cigarette Smoke by Hedera helix L. and Chlorophtum comosum variegaturn Hort. and Their Physiological Responses

JIN Ting-ting, JIN Meng-die, CHEN Zhen, CHENG Li-ling, ZHU Kai-xuan

2018, 57(18): 47-51. doi:10.14088/j.cnki.issn0439-8114.2018.18.011

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Hedera helix L. and Chlorophtum comosum variegaturn Hort. were set as the objects,which were put into a sealed glass capsule with length,width,height all of 60 cm. Cigarette smoking of mankind was imitated and two cigarettes were injected into the capsule. Changes of CO and PM2.5 concentrations during 48 h,phenotypes and physiological indexes at 48 h were determined,to investigate the purifying capacity of the two hanging plants to cigarette smoke and their growth and physiological responses. The results showed that H. helix and C. comosum variegaturn both could absorb CO and PM2.5 to some degree and purify the air in the sealed space. The purifying capacity per unit leaf area of H. helix was superior than C. comosum variegaturn. Meanwhile,growth of the two plants were affected by cigarette smoking treatments and leaf chlorosis and necrosis were appeared. After cigarette smoking treatments,levels of reactive oxygen species（including superoxide anion and hydrogen peroxide） in leaves of the two plants were significantly increased,resulting in the increases of malondialdehyde,the products of membrane lipid peroxidation. The plants activited peroxidase or synthesized proline to resist the damages and the resistance of H. helix was better than C. comosum variegaturn.

Study on the Meterological Background of Procambarus clarkii’s Disease in Hubei Province

DENG Ai-juan, LIU Ke-qun, LIU Min, TANG Yang

2018, 57(18): 52-56. doi:10.14088/j.cnki.issn0439-8114.2018.18.012

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In order to investigate the meteorological cause of the occurrence of Procambarus clarkii’s disease in Hubei province in recent years, the climatic conditions during the breeding stage of the main cultivation areas in Hubei were analyzed. The results showed that the production of P. clarkia had some correlation with the time of cold air’s occurrence, the duration and degree of low temperature, and the amplitude of temperature in March to June in spring season. The more frequent the cold air activities occurred, the more duration days of low temperature continued, the lower the temperature level was, and the greater the magnitude of temperate fluctuated, then the more serious the disease would occur, and this could be reflected in the production of the year. Therefore, the monitoring and forecasting onto the meteorological conditions in spring could provide decision making services for the cultivators of P. clarkia, in order to remind to take timely prevention and control measures to reduce the probability or extent of disease occurrence.

Experiment Research on Coal Chemical Industry Excess Sludge Reduction Based on Ultraviolet Irradiation

ZHENG Lan-xiang, CHEN Wei-min, LI Gong

2018, 57(18): 57-59. doi:10.14088/j.cnki.issn0439-8114.2018.18.013

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The coal chemical industry sludge is solid waste which produced in the process of coal chemical industry wastewater treatment. On the basis of analyzing the basic characteristics of coal chemical industry sludge, the effects of coal chemical industry sludge reduction with ultraviolet irradiation was carried out. The experimental results showed that after 0 min,10 min,30 min,60 min after UV irradiation, the concentration of TN, TP in coal chemical excess sludge increased because of cell rupture. And due to available to other microorganisms, the concentration of COD showed a trend of increase after decreases first. And the concentration of SS gradually reduced after UV irradiation. So, it can obtain certain coal chemical industry excess sludge reduction using ultraviolet radiation. To provide reference for Ningdong coal chemical excess sludge reduction, it need to further strengthen the experimental and applied basic research on the sludge reduction.

Selecting of Antagonistic Bacillus against Tobacco Black Shank and Detecting of Control Efficacy

SHI He-li, TAN Jun, DING Cai-fu, ZHAO Xiu-yun, XIANG Bi-kun, LUO Fang

2018, 57(18): 60-65. doi:10.14088/j.cnki.issn0439-8114.2018.18.014

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In order to control the tobacco black shank caused by Phytophthora parasitica var. nicotianae,42 bacterial strains were isolated from different health status tobacco rhizosphere soil,10 of them had an antagonistic effect on P. nicotianae by using the plate confronting method and treatment with fermentation filtrate after heated at 115 ℃. Three groups of Bacillus could be identified under the 16S rDNA sequence analysis：Bacillus amyloliquefaciens, B. subtilis and B. velezensis. The characteristics of cellulase and protease activity,colonization of Bacillus in tobacco rhizosphere soil,root and stem,and field experiments were carried out on B. subtilis GJ1,B. amyloliquefaciens GJ7 and B. velezensis GJ11. The results showed that GJ1,GJ7 and GJ11 strains could secrete cellulase and protease,and colonize in the rhizosphere soil,root and stem of tobacco. The ability of colonization in the rhizosphere soil was GJ1>GJ7>GJ11,in root and stem was GJ7>GJ1>GJ11. However,the control efficiency against tobacco black shank of GJ1,GJ7 and GJ11 were 33.33%,80.00% and 28.60%,respectively,suggesting that the control efficacy in field of antagonistic Bacillus may depend on its colonization ability in tobacco.

Comparison of Control Effect of New Microbial Fungicide with Chemical Pesticides against Aphis gossypii Glover

ZHANG Hua-chong, YAN Zhen-hua, ZHAO Shu-qi, HUANG Xiao-li, DAI Bao-sheng, LI Wei

2018, 57(18): 66-68. doi:10.14088/j.cnki.issn0439-8114.2018.18.015

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In order to realize the technology of green prevention and control, the effect of new environmental-friendly microbial fungicide Yabijing against Aphis gossypii was explored, compared to two chemical pesticides （imidacloprid and chlorpyrifos）. Field tests indicated that Yabijing, imidacloprid and chlorpyrifos showed high control efficacy. The control effect maintaine up to 98% after 5~15 days of microbial fungicide Yabijing application, and it had significant difference with the control effect of 10% imidacloprid and 40% chlorpyrifos application. The control effect of 10% imidacloprid and 40% chlorpyrifos application began to decrease and declined greatly after 15 days which resulted in increasing the quantity of A. gossypi.

The Technical Measures for the Control of Weeds in the Rice Field of Direct Seeding

JIN Hong-mei

2018, 57(18): 69-70. doi:10.14088/j.cnki.issn0439-8114.2018.18.016

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According to the rice planting or leaf age and types of reference weeds and weeds size,the types and targeted methods of using chemical herbicides were listed; Before sowing,closed chemical control was mainly used,and the comprehensive prevention control technical measures of "one closed,two kill and three supplement" were mainly adopted after broadcasting.

Study of Weed Control with 3% Florpyrauxifen EC in the Direct Seeding Rice Field

ZHANG Jian-ping, ZHOU Zhi-zhong, TANG Wei, YU Xiao-yue, LU Yong-liang

2018, 57(18): 71-72. doi:10.14088/j.cnki.issn0439-8114.2018.18.017

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The effect of 3% florpyrauxifen EC on weed control in direct seeding rice field was studied. The results showed that 3% florpyrauxifen EC could effectively control a broad spectrum of weeds in the direct seeding rice filed. The weed control efficiency of Euphorbia lathyris,Ammannia arenaria,Polygonum hydropiper,Lindernia procumbens and Cyperus rotundus were totally up to 100% at 35 d by 36 g a.i./hm² 3% florpyrauxifen EC or 27 g a.i./hm² 3% florpyrauxifen EC mixed with 10% cyhalofop-butyl EC （112.5 or 225.0 g a.i./hm²）.

Study on Seed Stratification Technology of Idesia polycarpa

CHEN Yao-bing

2018, 57(18): 73-75. doi:10.14088/j.cnki.issn0439-8114.2018.18.018

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4 types of Idesia polycarpa Maxim seeds, the local wild type, the artificially cultivated yellow fruit type, the grafted red fruit type, and the artificially cultivated red fruit type, after removing the wax layer, were used as materials to compare the different layering time on generation in order to obtain the best stratification time and type. The result showed that, comparing the effects of different stratification treatment time on seed germination rate, the germination rate of four types of yam seeds increased with the stratification time in the stratification time of 60 to 90 d. Compared with the effects of accumulation time on different types of germination rate, the local wild type had the highest germination rate; and the germination rate from low to high was grafted red fruit type, artificial cultivation red fruit type, artificial cultivation yellow fruit type, and local wild type.

Analysis on the Biochemical Components in the Spring Shoots of Tea Cultivars

GAO Chang-qing, YI La-mei, TANG Ya-dong, YANG Xin-he

2018, 57(18): 76-79. doi:10.14088/j.cnki.issn0439-8114.2018.18.019

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The biochemical components including water extracts,caffeine,free amino acid,theanine,poly phends and catechin in the one bud and two leaves of spring shoots of 11 tea[Camellia sinensis （L.） O. Ktze.] cultivars were analyzed;and the ratio of tea poly phenols and amino acid was calculated. The results showed that the content of water extracts was 45.56% to 53.56%;the content of caffeine was 24.60 to 38.64 mg/g;the content of free amino acid was 25.17 to 79.80 mg/g;the content of theanine was 2.78 to 31.10 mg/g;the content of tea poly phenol was 127.30 to 242.22 mg/g;the content of catechin was 85.52~177.21 mg/g;and the ratio of tea poly phenols and amino acid was 1.60 to 9.62. The content of the biochemical components in the one bud and two leaves of spring shoots of different tea varieties is different. In comparison, Anji white tea,Duokangxiang,Shuchazao and Xianyuzao have obvious biochemical characteristics of high amino acid content and low tea polyphenol content,are more suitable for making high quality green tea.

Species Abundance Distribution Model of Keteleeria davidiana Community in Zhangheyuan Nature Reserve

ZHANG Min, ZHANG Xiao-lin, WANG Yang, LI Xin-zhi

2018, 57(18): 80-83. doi:10.14088/j.cnki.issn0439-8114.2018.18.020

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Based on model octave grouping method, Weibull distribution model was employed to fit the species abundance of the tree layers in the Keteleeria davidiana（Bertr.） Beissn. community in Zhangheyuan Nature Reserve of Nanzhang county, Hubei province. The results showed that, the Weibull distribution models of the tree layers of 3 consecutive sample plots and 3 samples plots combined were tested with ², and the fitting models of tree layers in sample plot 1 and sample plot 3 were in accordance with Weibull distribution, while the fitting models of tree layers in sample plot 2 and the sample plots combined were not completely in accordance with Weibull distribution; the K-S test showed that the species abundance of the tree layers was consistent with Weibull distribution through four sampling sources. Comprehensively speaking, Weibull distribution model proves to be applicable to the species abundance distribution of tree layers in K. davidiana communities.

Study on Mathematical Model of Comprehension Cultural Measure for High-Yield of Artemisia Annua Seed in Yellow Soil

CUI Guang-lin, LI Long-yun

2018, 57(18): 84-87. doi:10.14088/j.cnki.issn0439-8114.2018.18.021

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Experiment was done in yellow soil of Chongqing city. Quadratic regression universal rotary combination design experiment of four factors and five levels was used to study the effects of different density N,P,K fertilizer on seed yield of current representative Artemisia annua varieties Yuqing No.1. Optimum formula was found by simulated equation and optimality selection. The results showed that,planting density biomass and dose of N,P,K all had a significant regression relationship with seed yield. The effect was density,N,P,K in descending order. The best design was that density at 20 000 plant/hm²,N fertilizer of 250 kg/hm², P fertilizer of 750 kg/hm², K fertilizer of 75 kg/hm², it could attain a maximum seed yield of 743 kg/hm².

Effects of Aposter Combined with Canthaxanthin on Antioxidant Function, Egg Quality and Yolk Pigment Deposition in Laying Hens

LI Jun, CHENG Ye-fei, SU Yue, TAO Zheng-guo, ZHOU Di, ZHOU Yan-min, WEN Chao

2018, 57(18): 88-91. doi:10.14088/j.cnki.issn0439-8114.2018.18.022

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A total of 270 54-wk-old Hy-Line Brown laying hens were allocated into 3 treatments with 6 replicates of 15 hens each. Hens in the control group were fed a basal diet, and hens in the test Ⅰ and Ⅱ were given the basal diet supplemented with 5.0 mg/kg apoester combined with either 2.5 or 5.0 mg/kg canthaxanthin, respectively. The results suggested that compared with the control group, hens in test Ⅰ exerted a decrease in malondialdehyde accumulation in the liver（P<0.05）. Treatments increased values of Roche colorimetric fan, redness, and yellowness, and contents of canthaxanthin and lutein, but reduced lightness value in the egg yolk（P<0.05）. The supplementation of apoester combined with canthaxanthin could improve color and pigment accumulation in the egg yolk, and combination of 2.5 mg/kg canthaxanthin with 5.0 mg/kg apoester could enhance antioxidant capacity in the liver of laying hens.

Epidemiological Investigation of Chlamydia Antibody in Cattle and Sheep in Hubei Province

ZHOU Wen, PENG Fu-hu, HU Si-shun, ZHANG Hai-yan, XIAO Hong-de, LIU Yang-yang, LI Zi-li, BI Ding-ren

2018, 57(18): 92-93. doi:10.14088/j.cnki.issn0439-8114.2018.18.023

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In order to understand the epidemic situation of Chlamydia in Hubei Province,380 serum species from 40 farms （18 cattle and 20 sheep or goat） in various regions of Hubei province were collected tested by indirect hemagglutination test （IHA）. The results showed that the total positive rate was 44.21%（168/380）. All regions in Hubei province were found positive of Chlamydia antibody detection. The infection rate of cattle was relatively high,and the positive rate in some farms was up to 100%. This survey showed that Chlamydia infection in cattle and sheep was common in Hubei province,and there were also regionality.

Segmentation Method for Plant Leaves Disease Based on Color Difference

JI Tian-tian, LI Ze-bing, ZHAO Jiang-dong, YAO You-feng, HUANG Ji

2018, 57(18): 94-97. doi:10.14088/j.cnki.issn0439-8114.2018.18.024

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In view of the complexity of plant leaves images, combining with the characteristics of plant color leaves images,a color image segmentation method based on color differences was presented. Firstly,the images of the diseased plant leaves were collected,use GrabCut algorithm to segment the complex field environment background. Then,use median filtering and image sharpening method to preserve the image of disease area and edge detail. Next,the processed images are converted to Lab color space and YUV color space which based on physiological characteristics. Combined with Otsu method,the images of Lab grayscale and single channel grayscale of YUV color space are subdivided respectively. Finally,the two subdivision of the two figures and operations,its conversion to RGB color space,you can get the final segmentation results. The method was used to segment the common color images of leaves diseases plants such as soybean,corn,rape and cucumber. The experimental results showed that this method can obtain more accurate segmentation,Edge detail protection and segmentation efficiency also have good results.

Design of Real-time Monitoring System for Cold Chain Logistics Temperature and Humidity of Fresh Agricultural Products Based on ARM9 and ZigBee

QIN Ning-ning

2018, 57(18): 98-102. doi:10.14088/j.cnki.issn0439-8114.2018.18.025

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The fresh agricultural products are easily rotted in the course of transportation because of the temperature,relative humidity and other reasons. It is necessary to improve the information degree of cold chain logistics of fresh agricultural products,so as to reduce the loss of fresh agricultural products during transportation,ensure the freshness of cold fresh and fresh agricultural products. Based on the special requirements of cold raw and fresh agricultural products for temperature and humidity,combined with advanced sensors and wireless transmission technology,a real-time monitoring system for cold chain logistics temperature and humidity of fresh agricultural products with ARM9 and ZigBee gateway as the core is designed in this paper.

Cloning and Expression Analysis of 3-dehydroquinate Synthase Gene in Pomegranate

YIN Yan-lei, TAO Ji-han, YANG Xue-mei, TANG Hai-xia, FENG Li-juan

2018, 57(18): 103-105. doi:10.14088/j.cnki.issn0439-8114.2018.18.026

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The 3-dehydroquinate synthase（DHQS） cDNA sequence was cloned from Taishanhong pomegranate（Punica granatum L.） pericarp with RT-PCR technique. The expression of DHQS gene was analyzed in different pomegranate cultivars fruits during development. The result showed that the cDNA sequence length of DHQS was 273 bp,which encoded 91 amino acids. The protein of DHQS gene contained DHQ_Fe_ADH superfamily conservative structure domain. The similarity of DHQS nucleotide sequence in pomegranate and Fagus sylvatica,Prunus mume,Medicago truncatula were 91%,87% and 88%,respectively. The similarity of DHQS deduced amino acid sequences in pomegranate and Fagus sylvatica,Malus domestica,Pyrus x bretschneideri,Arabidopsis thaliana were 95%,92%,92% and 88%,respectively. The relative expression level of DHQS gene decreased gradually with the fruit development in two pomegranate cultivars,which peak value appeared at July 15^th. The relative expression level of DHQS gene in Taishanhong was higher than that in Taishansanbaitian in the whole development period.

Study on Micro-tubulin Gene Ghtub12 to Cotton in Gossypium hirsutum

SHEN Zhe-nan, SHEN Qiu-ping, PENG Qiu, LYU Zun-fu, LI Fei-fei

2018, 57(18): 106-110. doi:10.14088/j.cnki.issn0439-8114.2018.18.027

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Cotton is the world's important fiber crop, cotton tubulin gene in cotton fiber development process specific advantages of expression, affecting cotton fiber quality. Tubulin gene Ghtub12 as a member of the tubulin gene family, the mechanism of cotton fiber development is not clear. In this experiment, R15 hypocotyls were used as acceptor, and 0.5 mg/L Basta was used as a screening marker. Agrobacterium tumefaciens-mediated transformation method was used to study the genetic transformation. 100 calli were induced by resistance callus, and 143 callus were obtained after 1 month. The recovery rate was 71.50%. After 3 times, the callus was obtained and the callus was obtained. The transformation rate was 81.00%. Among them, 45 callus induced embryogenic callus, the differentiation rate was 44.12%. Embryogenic calli were divided into 60 seedlings. The positive rate was 93.3%. The transgenic tubulin gene Ghtub12 was obtained, which provided experimental material for further study on the possible mechanism and effect of Ghtub12 gene in cotton.

The Present Situation of Talent Team Construction in Provincial Agricultural Research Institutes under the New Normal Condition——A Case Study of Hubei Academy of Agricultural Sciences

ZHOU Li, TU Jing, DONG Jian-hui, WEN Liang, FU You-zhi, LIU Lan-yu, SHENG Min, LIU Jie

2018, 57(18): 111-114. doi:10.14088/j.cnki.issn0439-8114.2018.18.028

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Talent resources are the first strategic resources. To strengthen the talent team construction is the fundamental factor to promote an all-round development of agricultural science and technology. Through introduction on the present situation of agricultural talent team construction in Hubei Academy of Agricultural Sciences, the existing problems were analyzed, and then the countermeasures and suggestions for strengthening the talent team construction were put forward.

Optimization of Urban Land Use Structure Based on GLP Model——A Case Study of Hohhot

TIAN Tong-yu, YU Mei, SU Hong

2018, 57(18): 115-120. doi:10.14088/j.cnki.issn0439-8114.2018.18.029

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Taking the municipal districts of Hohhot city as the study area, combined with the land use change data and social economic statistical data about the study area from 2009 to 2015, took some main factors for influence the structure optimization of city land use as the factors of optimal decision, respectively using GM（1,1） and GLP to estimate and construct the intervals of predictive value of the factors and inequality constraint equations, and respectively according to the interval prediction value of the limit to design two configuration optimization schemes of land use of the study area in 2020 and compared and optimized for it. The results show that compared to the optimization scheme of the interval of lower value, optimization scheme of the upper limit value in the efficiency of the whole land output and single type of land scale and combination was better than the optimization scheme of lower limit and current structure of land use structure.

The Effect of Sensory Experience on Farmers’ Willingness of Agricultural Materials Online Shopping at Agency Service Stations

LI Lin-zhu, XU Guang-lan, LI Yan-jun

2018, 57(18): 121-124. doi:10.14088/j.cnki.issn0439-8114.2018.18.030

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Based on the actual situation of the running of agricultural materials e-commerce, this paper aims to explore the effect of farmers’ offline sensory experience in the agency service stations on their willingness of agricultural materials online shopping at agency service stations. The results show that farmers’ offline sensory experience has a positive impact on their willingness of agricultural materials online shopping at agency service stations, in which perceived risks play a mediating role. In addition, the perception of interpersonal trust strengthens the decreasing effect of sensory experience on perceived risk to some extents. The need of cognitive closure strengthens the positive effect of sensory experience on farmers’ willingness of agricultural materials online shopping.

The Risks of Manure in Agricultural Industrialization——the Case of Germany

KIM PHILIP Schumacher, SU Yong-wei, MARTIN Franz, ZHANG Yue-qiang

2018, 57(18): 125-127. doi:10.14088/j.cnki.issn0439-8114.2018.18.031

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Germany is a country with a highly developed agriculture, but agricultural industrialization resulted in growing amounts of animal manure spread on the fields due to the expanding scale of animal breeding. This development results in serious environmental pollution which is not only risky for the people's health but also for the future development of production. Therefore, the article introduced the basics of agricultural industrialization in Germany, gave an example for identifying the risks related to animal manure in agricultural industrialization in the area of the Oldenburger Muensterland and gave recommendations for action aiming at reducing the risks of manure in agricultural industrialization.

The Development Planning for Xianning Ramie Industrialization

XIONG Wei, TANG Di-luo, PAN Guo-xiong, QU He-ping, FU Cong, ZHU Wei

2018, 57(18): 128-130. doi:10.14088/j.cnki.issn0439-8114.2018.18.032

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Ramie was one of main characteristic agricultural products in Xianning city, which has owned a large-scale industrial cluster,but the products are most primary processed products,which limited the development of ramie industry. For the combination of goverment,production and sales enterprises,research centers and other industries,some countermeasures were put forward for the healthy development of ramie industry in Xianning.

The Suburban Rural Women Employment Security in Construction of Characteristic Small Town——Based on Stakeholders Theory

WU Rong

2018, 57(18): 131-136. doi:10.14088/j.cnki.issn0439-8114.2018.18.033

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The difficult issue of suburban rural women employment in construction of characteristic small town was analyzed based on the stakeholder theory,it was discovered that, the difficulty of employment for suburban rural women was resulted from the different interests among the government,suburban rural women,women's organizations,regulations and policies on gender equality assessment advisory committee,enterprises,universities and research institutions and other stakeholders.It was suggested that all stakeholders should give full cooperation to form a social joint force,thus to ensure the realization of employment rights and interests for the suburban rural women.

Countermeasures and Suggestions to Enhance the Actural Effectiveness of Rural E-commerce Poverty Alleviation——Based on An Investigation of Shangluo,Ankang and Hanzhong in Shaanxi Province

LI Cai-xia, YANG Wei-peng

2018, 57(18): 137-140. doi:10.14088/j.cnki.issn0439-8114.2018.18.034

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Taking Shangluo city,Ankang city and Hanzhong city of Shaanxi province as examples,the current situation of e-commerce poverty alleviation was analyzed,its main problems were found out,and then a precise e-commerce poverty alleviation strategy centering on cultivating characteristic industries and e-commerce ecology was put forward.

Development Path of Protecting Agricultural Culture Heritage in Huzhou City

XIONG You-ping

2018, 57(18): 141-144. doi:10.14088/j.cnki.issn0439-8114.2018.18.035

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In response to the national strat egies of “Rural Revitalization” and “Beautiful China”, it is not only to put an end to Authenticity but also to inherit living and to care complexity by fully tapping the value of agricultural culture heritage and exploring protection tourism development in the aspect of landscape and tourism. In order to achieve the all-win harmonies between historical value and tourism development,and heritage protection and economic output, the concrete suggestions in Huzhou city include to explore the agricultural culture heritage by establishing a mechanism and finding out the real situation,to protect it by industrial promotion and standardized management, and to inherit it by balancing interest and being driven by research.

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