Component Characteristics of Amino Acids and Organic Acids in Root Exudates from Transgenic Bt Cotton at Different Growth Stages
ZHAO Yue, XI Rui-ze, FU Qing-ling, ZHU Jun, HU Hong-qing
2018, 57(4):
26-30.
doi:10.14088/j.cnki.issn0439-8114.2018.04.007
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The component characteristics including organic acids, amino acids and Bt proteins of root exudates at different growth stages （seedling, seedling stage and bud stage） from transgenic Bt cotton and conventional cotton were studied. The results showed that the pH of transgenic Bt cotton and common cotton root exudates decreases gradually in the growth period, from the alkaline in seedling stage （>8.0） to the subacidity of the bud stage （<6.8）; The water-soluble carbon,water-soluble nitrogen, Bt proteins, organic acids, amino acids and total soluble sugar were increased significantly with the extension of growth period. At the same growth stages, there were no difference in main components of the two cotton root exudates, but the most root exudates component of conventional cotton（such as the water-soluble carbon, water-soluble nitrogen, total soluble sugars, amino acids, organic acids, etc.） were higher than those in Bt cotton, especially in the bud stage. As the main organic acid in the root exudates, formic acid in transgenic Bt cotton root exudates accounted for 55%, 75% and 93% at the young seedling, seedling and bud stages, respectively, and that in conventional cotton root exudates accounted for 65%, 39% and 96%, respectively. Therefore, the content of the formic acids was the main difference in the organic acids between the conventional and transgenic Bt cottons. As one of main amino acids in root exudates, threonine in the transgenic Bt cotton and conventional cotton at the bud stage accounted for 42% and 36%, respectively. Compared to the transgenic Bt cotton, the conventional cotton root exudates could provide more carbon and nitrogen resources for plant growth.
