HUBEI AGRICULTURAL SCIENCES

Advances in studies of transcription factors in rice related to tapetum development

ZHANG Qiu-yun, SHEN Ya-qi, JIANG Wen-xiang, LIU Jia-lin, WANG Lian-hong, HE Hao-hua, HU Li-fang

2021, 60(19): 5-10. doi:10.14088/j.cnki.issn0439-8114.2021.19.001

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Tapetum is the innermost structure of anther wall in rice. It is the provider of enzymes and sporopollen for the formation of fertile pollen grains. It is of great significance to regulate pollen fertility. At present, a series of transcription factors involved in the development of rice tapetum have been isolated, which are involved in the development of tapetum. In this paper, the molecular mechanism and regulatory network of transcription factors such as MADS, bHLH and PHD finger related to rice tapetum development were summarized, which laid a foundation for further understanding the molecular mechanism of rice tapetum development.

Research progress and prospect of orchard transportation equipment and digital orchard

WANG Zuo, ZHANG Jun-feng, XIAO Jin, LUO You-yi, TIAN Man-zhou, HE Yu-shuang, ZHOU Zhao

2021, 60(19): 11-14. doi:10.14088/j.cnki.issn0439-8114.2021.19.002

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By means of literature investigation, the research results of domestic and foreign scholars on orchard transportation equipment and digital orchard and the actual application of the products were summarized. The research and application status of mechanized orchard transportation equipment at home and abroad and the research status of digital orchards in China were summarized, the main problems were analyzed, and the development trend of orchard transportation equipment and digital orchards was prospected.

Advances in the study of Acanthopanax trifoliatus（L.） Merr. and their relatives in the same genus

GUO Yong-xiu, LI Fang-chan, SUI Jia-ning, NONG Yi-qing, JIANG Lin

2021, 60(19): 15-18. doi:10.14088/j.cnki.issn0439-8114.2021.19.003

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Acanthopanax trifoliatus（L.） Merr. is a common folk medicinal plant in Guangxi Zhuang Autonomous Region. The chemical constituents and pharmacological effects of Acanthopanax senticosus and its related plants were summarized, the commonness and individuality in Acanthopanax senticosus and its related plants were discussed, and its development prospect was prospected, so as to provide reference for the further development and utilization of Acanthopanax senticosus and the establishment of quality evaluation.

ARTP irradiation on pollen grain of Brassica napus and phenotype identification of their offsprings

HE Xiao-ying, LU Wen-hu, SHI Wei-ce, WANG Wen-min, WANG Jing-qiao, CHEN Wei, ZHANG Guo-jian, ZHANG Jian-kun, SHU Zheng-qi, ZU Feng

2021, 60(19): 19-22. doi:10.14088/j.cnki.issn0439-8114.2021.19.004

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In order to obtain the mutant lines of high oil content rape, the ARTP mutation experiment of pollen grains of Brassica napus was carried out.The pollen of the restoring line 16C of Ogu CMS in Brassica napus was irradiated with ARTP mutation technology for 0, 20, 30 and 40 min respectively, and the mutated pollen was given to the sterile single plant 16CS naturally produced in the same population. Then traits of the progeny, such as seed number per pod, seed germination rate and energy and oil content were surveryed. The results showed that ARTP irradiation of Brassica napus pollen for more than 30 min could significantly reduce the number of single horned grains at the time of mutagenic pollen pollination and the germination potential and germination rate of M₀ generation seeds, improve the sterile plant rate of M₀ generation plants and the oil content of M₁ Generation seeds, and obtain the mutant line ARTP30-315 with an oil content of 52.61%. The preliminary results showed that it was effective to carry out rape pollen mutation by ARTP-P instrument, and the more suitable ARTP irradiation time was 30~40 min. A more suitable parameter system was explored for carrying out pollen mutation of Brassica napus by ARTP-P instrument in production, which provided a new method and idea for carrying out germplasm innovation of Brassica napus in the future.

Breeding and high yield cultivation technique of fresh maize variety Guitiannuo 228

LIU Ya-li, LU Sheng-qiao, CHEN Kun, GONG Xue, SU Qi, TENG Hui-sheng, YANG Yao-jiong, ZHONG Chang-song, ZHANG Shu-kuan

2021, 60(19): 23-24. doi:10.14088/j.cnki.issn0439-8114.2021.19.005

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Guitiannuo 228 is a new sweet waxy corn variety bred by the Maize Research Institute of Guangxi Academy of Agricultural Sciences. It was bred by the waxy inbred line 518MBC-13 as female parent and wxwxsh₂sh₂ inbred line 6 ZhenBCW-2-15 as male parent. It was approved by Guangxi Crop Variety Approval Committee in 2020. The variety has high edible quality and excellent commodity properties, high yield, strong disease resistance and wide adaptability. Because the ratio of waxy and sweet kernels of F2 is 3∶1, which takes into account the flavor of waxy corn and sweet corn and the taste of consumers in North and South China,it has become an important type of fresh corn in China.

Effect of reducing rice planting area on water environment in Ningxia irrigation area

ZHENG Fang, MA Li-wen, ZHAO Hui

2021, 60(19): 25-29. doi:10.14088/j.cnki.issn0439-8114.2021.19.006

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From the perspective of transporting water to the air, the adverse effects of reducing rice planting area in Ningxia irrigation area were analyzed. The results showed that reducing the rice area of 10 000 hm², half of which was changed to corn area and the other half to wheat area, the water evapotranspiration would reduce by 3.032×10⁷ m³. Under the condition of concentrated continuous planting and calm wind, the air relative humidity would decrease by 11.6% from April to September. If the irrigation area reduced rice area by 10%~30% and changed to corn area and wheat area, the water transported to the air in the whole irrigation area would reduce by 2.5×10⁷~7.4×10⁷ m³, the groundwater level droped by 29.3~87.8 mm every year. It could be seen that the contribution of maintaining rice area to ecology was huge.

Breeding and application of rapeseed variety green manure Zhongyoufei 1

QIN Lu, LIAO Xiang-sheng, ZHENG Yuan-ben, XIE Li-hua, GU Chi-ming, LIAO Xing

2021, 60(19): 30-33. doi:10.14088/j.cnki.issn0439-8114.2021.19.007

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Zhongyoufei 1 was a new rapeseed variety which could use for green manure or oilseed production which was bred by the oil crop research institute of Chinese academy of agricultural sciences. The results of 2-year multi-site experiment in Hubei province showed that, under low fertilizer input conditions, the fresh weight of shoot biomass was 43 560.0 kg/hm² on average, which was 15.81% increase than that of the control variety Yangguang 2009, the amount of N, P（P₂O₅） and K（K₂O） returning were 88.20 kg/hm²,14.25 kg/hm² and 119.10 kg/hm², increasing by 21.2%, 12.5% and 17.3%. The seed yield of Zhongyoufei 1 was slightly more than that of the control cultivar under low N fertilizer input conditions, and its seed quality meeted the criteria of double low rapeseed in China. Under normal fertilizer application, the variety could be used as seed production and green manure.

Soil quality monitoring and diagnosis of pepper and Chinese cabbage rotation system under different fertilization treatments

WANG Su-ping, DU Lei, HONG Juan, HUANG Xiang, ZHANG Gui-you, JIANG Li, CHENG Wei-shun, LUO Xi, ZHANG Li-hong, YE Li-xia, CHEN Gang

2021, 60(19): 34-38. doi:10.14088/j.cnki.issn0439-8114.2021.19.008

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The effects of different fertilization treatments on crop yields and soil physical and chemical properties of pepper-cabbage rotation system were studied. The results showed that fertilization could increase the yield of pepper and Chinese cabbage, and the yield of 2/3 chemical fertilizer nitrogen+1/3 organic fertilizer nitrogen treatment was high, which was 7 808 kg/hm² for pepper and 65.18 t/hm² for Chinese cabbage. Fertilization could improve soil physical properties, cultivation and application of organic fertilizer could reduce soil bulk density, and application of organic fertilizer could increase soil water content. After fertilization, the activity of urease, alkaline phosphatase and invertase in the soil were all increased. There was no significant difference among soil invertase and catalase activity, pH, and organic matter content in different fertilization treatments. Soil EC, mineral nitrogen, and available phosphorus and the content of available potassium were significantly increased. Considering crop yield, soil physical and chemical properties, fertilizer utilization rate and soil enzyme activity, 2/3 fertilizer nitrogen+1/3 organic fertilizer nitrogen was recommended.

Effects of rainfall and feed ramie on water loss and biomass of sloping farmland

XIONG Wei, XU Min, WANG Wei, TANG Di-luo, WANG Hong-wu

2021, 60(19): 39-42. doi:10.14088/j.cnki.issn0439-8114.2021.19.009

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The effects of rainfall and feed ramie planting on water loss and biomass of sloping farmland in 2018 were studied. The results showed that ramie grew from April to October, with more water loss in plum rain season and less water loss in autumn drought. Ramie was better than maize in reducing water loss. The rainfall of 40.0 mm was the starting point of water loss, and 62.3 mm was the out-of-control point of water loss. When the coverage reaches 90%, the amount of water loss could be reduced, and when the coverage was below 60%, the amount of water loss increased with the increasing of rainfall. The growth rate and daily weight gain of ramie were consistent with the average daily rainfall. Fresh grass for ramie was heavier than corn.

Potential evaluation and reuse scheme of plow layer soil-stripping of cultivated land occupied by non-agricultural construction in Nanchang city

LIU Yun, LI Hai-feng, TU Dai-hao

2021, 60(19): 43-48. doi:10.14088/j.cnki.issn0439-8114.2021.19.010

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Using the method of GIS spatial analysis and sampling survey, the quantity and scale of stripped arable soil in Nanchang city were calculated and analyzed. The results showed that in theory, the total area of stripable topsoil soil in Nanchang was 8 947.12 hm², and the amount of stripable earthwork was 2 387.42×10⁴ m³;The actual stripable cultivated land area of the cultivated layer to be occupied by non-agricultural construction in Nanchang was 7 036.94 hm², and the stripable soil earthwork was 1 958.95×10⁴ m³; The total area of cultivated land resources to be supplemented in Nanchang was 15 091.01 hm², and the total demand for covering soil was 4 527.31×10⁴ m³. The demand for covering soil of the newly-increased cultivated land from dryland to paddy consolidation was in big percentage （53.1%） of the total demand for covering soil of the newly-increased cultivated land in Nanchang. Dry land to paddy consolidation for the quality imprvoment of cultivated land,land consolidation for the development of cultivated land-reserve resources, and urban green space reconstruction were the main directions of reuse scheme of plow layer soil-stripping of cultivated land occupied by non-agricultural construction in Nanchang. Cultivated land occupied by non-agricultural construction abounds with strippable topsoil soil resource, which could meet the needs of more than 40% of the newly-increased cultivated land in Nanchang city.

Flooding height distribute of vascular plants in the water-level fluctuating zone of the Three Gorges Reservoir and influencing factors for its stability

LIU Ming-zhi, NIU Han-gang, LIN Feng

2021, 60(19): 49-54. doi:10.14088/j.cnki.issn0439-8114.2021.19.011

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In order to explore the impact of water storage and flooding on the vegetation in the water-level-fluctuating zone of the Three Gorges Reservoir reservoir, a survey of the vascular plants in the water-level-fluctuating zone was carried out by using the transect method from June to July 2012. The results showed that there were 81 species of vascular plants in the water-level-fluctuating zone, including 44 species in Chongqing Changshou-Banan section, and 65 species in Chongqing Fuling-Yunyang section, which were mainly herbaceous plants; Mainly gramineae and compositae. The dominant species were perennial submerged-tolerant plants such as Cynodon dactylon, Hemarthria altissima and Alternanthera philoxeroides; Plants were affected by human factors such as flooding, shipping, farming, and natural factors such as terrain and soil.

Study on the adsorption performance of Ganoderma lucidum bran on heavy metal Cu（Ⅱ） in water

RAO Yi-ping, WEI Jian-hua, YE Xiang-yu, YU Hai-qiao, ZHANG Shu-yi

2021, 60(19): 55-58. doi:10.14088/j.cnki.issn0439-8114.2021.19.012

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Ganoderma lucidum bran was used as the adsorption material, by changing the factors such as the dosage of bran, pH value, Cu（Ⅱ） concentration, adsorption time and temperature, the adsorption of Cu（Ⅱ） was studied. The results showed that Ganoderma lucidum bran had a good adsorption property for Cu（Ⅱ） in water, and it could be used in practical treatment of wastewater containing Cu（Ⅱ）. The best conditions for the adsorption of Ganoderma lucidum bran to Cu（Ⅱ） were the dosage of 30 g/L, pH=6, adsorption time of 90 min, and temperature of 35 ℃. The adsorption process accorded with Langmuir isothermal adsorption model.

Biodiversity analysis of phytoplankton and water quality evaluation in Baiyangdian Wetland

CHEN Jia-lin, KONG Fan-qing, YU Hai-jun, HUANG Yan-feng, SHI Wen-hui, HUANG Xin, LI Bing, WANG Qian

2021, 60(19): 59-64. doi:10.14088/j.cnki.issn0439-8114.2021.19.013

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In January, March, May, July, September and November 2018, samples were taken from five sampling sites in Baiyangdian to investigate the flora composition, density and abundance of phytoplankton species, Shannon-Wiener index, Pielou index and Margalef index were used for biodiversity analysis and water quality evaluation. The results showed that there were 74 species of phytoplankton belonging to 7 phyla at the sampling sites, among which diatom and green algae were dominant, the species of phytoplankton were abundant, and the water quality was moderately polluted according to the comprehensive evaluation.

Comparative analysis of mixed severe convective weather processes in spring and summer in Hunan

WANG Qi-huan, YAO Rong, ZHOU Chang-qing, WANG Qiang, ZHANG Kun

2021, 60(19): 65-74. doi:10.14088/j.cnki.issn0439-8114.2021.19.014

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Based on the conventional observation data, NCEP reanalysis data and Doppler radar data, the mixed severe convective weather on March 20, 2019 in spring （referred to as "March 20"） and August 21, 2019 in summer （referred to as "8·21"） in Hunan province was compared and analyzed. The results show that "3·20" was the warm advection forcing type under the westerly belt system, which brought hail and short-term heavy precipitation, and "8·21" was the quasi barotropic type at the edge of subtropical high, which brought thunderstorm gale. There was no obvious intersection of cold and warm air in "3·20" and no significant deep vertical wind shear in "8·21", which easily led to the neglect of severe convection in the forecast. The two processes had two kinds of sonde curves of upper dry and lower wet type and mixed type respectively, and the difference of stratification characteristics had different indicative significance. Compared with "8·21", "3·20" had better water vapor transport conditions. Both thermal conditions had the characteristics of low warm and humid convection unstable layer and upper dry and cold air intrusion. In terms of radar characteristics, both produced supercell storms, there were characteristic differences such as three body scattering, high hanging strong echo, high radial velocity area, middle-level radial convergence, which indicates that hail or thunderstorm wind was the main. The similarity of mixed strong convection in spring and summer was the focus of prediction work, which played a key role in the forecast and early warning of the main types and falling areas.

Infrared spectroscopy analysis of branches with different lignification degrees from tree peony

LI Xiu-li, CHEN Zhen, JI Xiao-mei, DAI Zhi-gang, CHEN Fa-zhi, CHEN Zhi-wei, ZHAI Jing-hua

2021, 60(19): 75-80. doi:10.14088/j.cnki.issn0439-8114.2021.19.015

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Both FTIR （Fourier Transformation Infrared Spectroscopy） and Guassian multi-peak fitting were employed to investigate the spectrum properties and the differences of protein secondary structure in perennial branch with the highest lignification degree （branch 1）, annual branch with higher lignification degree （branch 2）, annual branch with lower lignification degree （branch 3） of four years old Paeonia ostii ‘Fengdanbai'. The result of FTIR showed that 12 characteristic peaks were observed. The peak intensity at 1 253, 1 385, 1 461 and 1 738 cm^-1 became greater, indicating that the perennial branch contained more lignin; a blue-shift was observed in branch 1 and annual branches from 1 043 cm^-1 to 1 053 cm^-1, which showed that cell wall polysaccharides of branch with high and low degree of lignification were glucomannan and araban, respectively; a red-shift was occurred in branch 1 and branch 3 from 1 426 cm^-1 to 1 410 cm^-1, which indicated that the pectin of cell wall of branch 3 was combined with metal cation through monodentate complex. The result of Guassian multi-peak fitting for the amide Ⅰ region （1 700~1 600 cm^-1） indicated that branches contained β-sheet+side chain + pectin, β-sheet, α-helix, loop+turn, and β-sheet+turn of secondary protein structures at different degrees of lignification. Branch 3 with low lignification contained more β-sheet associated with pectin, which might regulate the cell wall self-assembly; branch 1 with highest lignification contained low amounts of α-helix might compensate for reduced protein-water hydrogen bond caused by lignification.

Landscape beauty evaluation of Kaifeng chrysanthemum flower show based on SBE method and stepwise regression analysis

TIAN Yu-hui, GUO Meng-di, SU Zhi-guo, LI Ang, SUN Li-qiang, WANG Zheng, WANG Peng-fei

2021, 60(19): 81-87. doi:10.14088/j.cnki.issn0439-8114.2021.19.016

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This study uses the SBE method to evaluate the beauty of the chrysanthemum exhibition landscape, decomposes different types of chrysanthemum exhibition landscape design elements through different professional groups, and selects 25 samples according to five types of elements to evaluate the beauty. The results show that different groups of people show differences in the degree of preference for the five elements. Through stepwise regression analysis, the correlation coefficients are 0.255 for artistic modeling, 0.217 for spatial layout, 0.195 for color richness, 0.193 for cultural mood, and 0.143 for fun. After returning, it is found that the relationship between the elements in the chrysanthemum exhibition is based on artistic modeling as the carrier, which gradually integrates and shows the characteristics of color richness, cultural artistic conception, spatial layout, and interestingness, and also reflects the characteristics and region of the chrysanthemum exhibition culture.

Study on the contents of total polysaccharides,total flavonoids,total saponins and the antioxidant activity in the extracts of leaves of Millettia speciosa Champ.

MO Hong-hui, DENG Guo-wei, LI Shan

2021, 60(19): 88-91. doi:10.14088/j.cnki.issn0439-8114.2021.19.017

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The extract of leaves from Millettia speciosa Champ. was prepared by hot water reflux extraction, and the contents of total polysaccharide, total flavonoids and total saponins in the extract were determined by colorimetric method combined with UV spectrophotometry. At the same time, the scavenging ability of DPPH·, ABTS⁺·, ·OH and O2-· free radical was determined to evaluate the antioxidant activity in vitro. The results showed that, the contents of total polysaccharide, total flavonoids and total saponins of leaves from Millettia speciosa Champ. were （1.36±0.05）%, （0.61±0.007）%, （0.15±0.013）%, respectively. The half inhibitory concentrations of DPPH·, ABTS+·, ·OH and O2-· free radical were 16.11, 4.78, 10.26 and 7.51 mg/mL. The contents of different types of components of leaves from Millettia speciosa Champ. were different, and the content of total polysaccharide was the highest. At the same time, it had good antioxidant activity in vitro, and had the strongest antioxidant effect on ABTS⁺· free radical.

Effect of different substratum formulations ratio on the growth and yield of Stropharia rugosoannulata

HAO Fu-xin, YANG Qing, LIU Xing-le, CHENG Xu, LI Li-yun, WANG Wen-jian, QI Sen-lin

2021, 60(19): 92-94. doi:10.14088/j.cnki.issn0439-8114.2021.19.018

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Using rice straw, corncob, corn straw as substratum,the effects of six different formulations ratio on mycelial growth, time of sporocarp appearance and yield of Stropharia rugosoannulata were studied to select the optimum formulations. The results indicated that with the ratio of 50% rice straw and 50% corncob, the yield of Stropharia rugosoannulata was the highest, reaching 11.88 kg/m², and the biological transformation rate was 95.01%.

Domestication and feeding evaluation of Dactylis glomerata L. in Yichang

XIONG Jun-bo, YU Wen-chang, SHEN Hong-xue, ZHU De-jiang, PENG Bing-xiang, XIONG Xiong, MAO Yu-nan, LI Yong, LIU Yang, CHEN Ming-xin

2021, 60(19): 95-97. doi:10.14088/j.cnki.issn0439-8114.2021.19.019

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In order to develop and satisfy the demand for native grass species for the healthy development of lawn industry in Hubei,from 2006 to 2017, the domestication, breeding and evaluation of wild orchardgrass（Dactylis glomerata L.） collected in Yichang area were carried out. A new strain of orchardgrass “Yichang” was bred by domestication and blending for three mixed selections, and the main agronomic traits and biomass of the original wild population were obviously improved. The new strain of orchardgrass from Yichang will have great potential for development and utilization in grassland establishment and grassland improvement in Hubei and even in south of China.

Effects of ramie whole plant pellet on growth performance,blood biochemistry and amino acid indexes of Hu sheep

WANG Wei, XU Min, XIONG Wei, ZHU Wei, WANG Hong-wu

2021, 60(19): 98-100. doi:10.14088/j.cnki.issn0439-8114.2021.19.020

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In order to study the effects of ramie[Boehmeria nivea （L.） Gaudich.] whole plant pellet on growth performance, blood biochemistry and amino acid indexes of Hu sheep, 18 male Hu sheep with similar body weight were randomly divided into 3 groups, with 6 sheep in each group. They were fed peanut rod granules, alfalfa granules and ramie granules respectively, and the concentrate was the same. The results showed that there was no significant difference in initial body weight and final body weight among the three treatment groups（P>0.05）. However, in terms of average daily gain, ramie pellet group decreased by 3.41% compared with alfalfa pellet group, and the difference was not significant（P>0.05）; But it was 42.82% higher than that of peanut rod granular material group（P<0.05）. In terms of blood biochemical indexes, there was no significant difference in serum total protein, albumin, globulin, total cholesterol, high-density lipoprotein and low-density lipoprotein among the three treatment groups （P>0.05）. In terms of muscle amino acid indexes of Hu sheep, there was no significant difference among the three treatment groups（P>0.05）, but ramie granular material had the trend of increasing mutton methionine content, which was 35.62% and 15.12% higher than alfalfa granular material and peanut rod granular material, respectively.

Quality standards of Zhuang medicine Psychotria serpens L.

HONG Ying-wen, XIE Ze-bi, LIANG Zi-ning

2021, 60(19): 101-105. doi:10.14088/j.cnki.issn0439-8114.2021.19.021

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The characteristics and microscopic identification of Psychotria serpens L. were studied. With asperuloside as the reference substance,TLC was applied in the qualitative identification of it. Moisture,total ash,acid insoluble ash and extractives in Psychotria serpens L. was determined based on the approaches in the appendix of Chinese Pharmacopoeia published in 2015,and the content of asperuloside was analyzed by HPLC. The results showed that the characteristics and microscopic identification of Psychotria serpens L. were described,with stable and reliable identification characteristics. The main spots in TLC were clear,with a good separation. The limits for moisture, total ash and acid insoluble ash in Psychotria serpens L. shall be less than 13.00%, 8.00% and 0.9%, respectively, and the limits for the extractives and the content of asperuloside shall be morethan 21.00% and 0.10%. The method established is simple, accurate and reliable, and the standard limit is reasonable, whic can be used to control the quality of Psychotria serpens L.

Comparing pH determination in cultivated land soil

HUANG Xiang, CHEN Gang, HONG Juan, DU Lei, CHENG Wei-shun, WANG Su-ping, LUO Xi, ZHANG Gui-you, JIANG Li, ZHANG Li-hong

2021, 60(19): 106-109. doi:10.14088/j.cnki.issn0439-8114.2021.19.022

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Soil acidity（pH） is one of the most important indexes of soil physical and chemical properties, which has an important effect on the nutrient form and availability of planting soil. In this research, international and national standards were used to measure soil pH in five cultivated land soils and the influencing factors were studied. We also discussed their difference between international and national standards. The results showed that the pH value was mainly affected by the ratio of soil and water, and the oscillation time and filtration had no significant effect on the determination results. There was a good correlation between the two standards. In addition, most of the test liquid was in a clear state after filtration, the stability time of the instrument test shortened, and the repeatability well.

The quality standards of traditional Chinese medicine preparations containing realgar are discussed in the 2020 edition of the Chinese Pharmacopoeia

ZHU Hua, YANG Wen-qi, HUANG Piao-ling, WEI An-da

2021, 60(19): 110-113. doi:10.14088/j.cnki.issn0439-8114.2021.19.023

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By sorting out the statistics of the content of traditional Chinese medicine preparations containing realgar, the amount of realgar taken daily and the quality standards, we discuss the quality standards of Chinese medicine preparations containing realgar, make suggestions for the safe use of traditional Chinese medicine preparations containing realgar, and provide reference for more scientific understanding and evaluation of traditional Chinese medicine preparations containing realgar.

Development of rain monitoring and alarm program based on Quartz.NET

LI Shu-jing, QING Guang-yong, WANG Xi-liang

2021, 60(19): 114-117. doi:10.14088/j.cnki.issn0439-8114.2021.19.024

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In order to obtain the precipitation data of the meteorological station from the Web server of the three-level forecast service platform of the Sichuan Meteorological Bureau, and to promptly remind the staff on duty to provide corresponding meteorological services when the precipitation value exceeds the prescribed threshold, C# Language was adopted, based on Quartz.NET developed a rain monitoring alarm program. The results show that the rain monitoring and alarm program based on Quartz.NET can not only monitor the accumulated precipitation data of various weather stations within a specific time period and within a specific area in real time, give timely alarms, but also automatically generate rain notifications required for decision-making weather services. The documentation greatly reduces the labor intensity of the staff, improves work efficiency, and plays an important role in the heavy weather service work during the flood season.

Design and research of animal epidemic disease dynamic monitoring system based on GIS in Shaanxi province

LI Wen-feng, YANG Ya-li, LI Long

2021, 60(19): 118-122. doi:10.14088/j.cnki.issn0439-8114.2021.19.025

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Based on GIS technology, the animal epidemic disease dynamic monitoring system in Shaanxi province was designed to realize macro animal epidemic prevention and dynamic monitoring on the basis of rapid development of animal husbandry informatization. At the same time, it provides basic data for the ecological development of modern animal husbandry. And finally realize digital management so as to promote the stable and healthy development of the province's information animal husbandry to provide scientific basis.

The virtual reality optimal design and research of Laoshan green tea processing

ZHANG Wen-da, DU Jian-wei

2021, 60(19): 123-126. doi:10.14088/j.cnki.issn0439-8114.2021.19.026

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Analysis of Laoshan green tea processing and virtual reality technology, the basic concept and application of 3 d modeling technology, to realize the Laoshan green tea processing and the whole process of informatization, digital interactive rendering, using virtual reality technology, 3 D modeling, process with Laoshan green tea processing, processing techniques, effective integration of design and analysis. This paper puts forward a tentative idea for the construction and application of the virtual reality system function module of Laoshan green tea processing, and explores the possibility of virtual reality technology to teach, popularize and optimize the application of green tea processing. It can enable users to experience green tea processing in the virtual environment, optimize the traditional teaching and scientific research methods of green tea processing, promote the dissemination of green tea cultural knowledge and processing technology promotion, promote the quality and efficiency of green tea products, realize the modernization and intelligence of agriculture, and contribute to the development and progress of virtual agriculture.

Design of farmland soil moisture monitoring system based on LoRa

ZHANG Jing-fang, ZHANG Ya

2021, 60(19): 127-129. doi:10.14088/j.cnki.issn0439-8114.2021.19.027

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In order to solve the problems of limited communication distance, large power consumption and no extensive network in soil moisture monitoring system, a field soil moisture monitoring system based on LoRa was developed, remote transmission, control and display of soil moisture monitoring information. The soil moisture data collected by the soil moisture sensor was uploaded to the gateway through the LoRa communication module, and the gateway collects the data and transmits it to the remote monitoring platform, a monitoring system of soil moisture in urban farmland with wide monitoring range, low power consumption, flexible network and long-distance communication was constructed. The system improves the working efficiency of soil moisture monitoring, and is very suitable for the scene where there are many data monitoring and collecting stations in large-area farmland.

Preliminary study of endophytic fungus diversity of tea leaf in an ancient tea garden,Jingmai,Yunnan,China

ZHANG Jian-kun, ZHOU Jian, PENG Chao, WANG Qin, WANG Fei, WANG Zhen-hua

2021, 60(19): 130-134. doi:10.14088/j.cnki.issn0439-8114.2021.19.028

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Tea leaf samples were collected from an ancient tea garden in Jingmai and Mangjing, Yunnan, China, and the naturally dried tea leaves were disinfected using 75% alcohol and 0.5% sodium hypochlorite and inoculated in PDA（Potato Dextrose Agar） plates for isolation of endophytic fungi. 103 fungal isolates were obtained, and basing on the morphological features 30 were selected for PCR and sequencing of ITS region and TEF gene. The results showed that 30 isolates were classified into 2 phyla, 3 classes, 7 orders, 10 families, and 13 genera, in which the isolates of Coprinellus spp. were the most （20.0%）, and Nodulisporium spp. （16.7%）, Hypoxylon spp. （13.3%）, and Daldinia spp. （10.0%） were behind in order. Comparing with reported references, the isolates in this study added taxonomic units of one order, four families and eight genera to the endophytic fungi in tea plant. Several isolates （such as 6, 10, 18, 21, 24, 26） were extremely possibly novel species of fungi. This study supposed that the ancient tea garden of Jingmai, Yunnan was quite different from usual artificially cultured tea gardens, and its endophytic fungi were extremely diverse and unique and deserved further investigation.

Recombinant expression and functional study of dextran binding protein in Apostichopus japonicus

JIA Chang-feng, CHEN Zhi-yu, LEI Yi-xuan, ZHANG Lei, KONG Ling-ming

2021, 60(19): 135-140. doi:10.14088/j.cnki.issn0439-8114.2021.19.029

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β-1,3-glucan-binding protein （GBP） is a pattern recognition receptor （PRR）, which can recognize pathogenic microorganisms and activate the immune response. The GBP gene （AJ-GBP） was cloned from the sequenced transcriptome of Apostichopus japonicus by Polymerase Chain Reaction （PCR）. Nucleotide sequence analysis showed that the total length of the gene was 660 bp, and it could encode 219 amino acids without signal peptide. The theoretical molecular weight of AJ-GBP protein was 24.64 kDa, and the isoelectric point was 5.94. It belonged to the glycosyl hydrolases family 16, and there were glycosylation sites at positions 17 and 193. The results of protein activity test showed that the recombinant protein had agglutination, binding and inhibition effects on Gram-positive and Gram-negative bacteria. It has obvious binding activity to glucan. These results suggest that AJ-GBP may be involved in the innate immune response of Apostichopus japonicus. This study laid a theoretical foundation for understanding the mechanism of resistance to invasion of pathogenic microorganism and the actual production and utilization of AJ-GBP recombinant protein.

Cloning and bioinformatic analysis of ERECTA gene from Solanum tuberosum

ZHANG Le, CHEN Ru-yi, LI Yu-shuang, GUO Zhi-ping

2021, 60(19): 141-144. doi:10.14088/j.cnki.issn0439-8114.2021.19.030

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ERECTA is one of the receptor-like protein kinase. It participates in the control of plant type and organ morphology, and plays an important role in stress resistance. In this study, potato （Solanum tuberosum） was used as the research object, and the cDNA sequence of potato ERECTA gene was amplified by RT-PCR. The results of bioinformatics analysis showed that the cDNA of ERECTA gene has a full length of 3 281 bp, including an open reading frame, which contains 2 973 bp, can encode 990 amino acids, and contains 10 conserved motifs. From the phylogenetic tree and multiple sequence comparison analysis, the amino acid sequence of potato ERECTA has high homology with other plant ERECTA amino acid sequences. The secondary structure analysis showed that potato ERECTA is dominated by α-helix and random coil. The three-dimensional structure prediction showed that potato ERECTA contains 8 leucine-rich LRR motifs. The results provide a theoretical basis for further research on the function of potato ERECTA.

Investigation on the practice mode of agricultural science and technology promoting industrial poverty alleviation in Hubei province：Taking Hubei Academy of Agricultural Sciences as an example

LU Guo-liang, LI Qiong-jie, WU Kang, YAO Ming-hua, LI Yue-ming, LIU Xiao-hong

2021, 60(19): 145-148. doi:10.14088/j.cnki.issn0439-8114.2021.19.031

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This paper expounds the typical mode of Hubei Academy of Agricultural Sciences using agricultural science and technology to promote industrial poverty alleviation, which has reference significance for further strengthening agricultural science and technology to promote industrial development, promoting the effective connection between poverty alleviation and rural revitalization. Through special investigation, this paper analyzes some shortcomings and problems of agricultural science and technology promoting industrial poverty alleviation, and puts forward some countermeasures and suggestions of agricultural innovation, promoting the development of agricultural industry, consolidating and expanding, preventing poverty return and promoting the all-round revitalization of rural areas.

Thinking on marketing strategy of Ankang selenium-rich tea

WANG Xuan-cheng, ZHAO Lin-long, YAN Qi

2021, 60(19): 149-152. doi:10.14088/j.cnki.issn0439-8114.2021.19.032

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Ankang tea has a history of more than 3 000 years. Ankang tea has unique characteristics and advantages because of its natural selenium rich. However, due to the influence of Dabashan green tea, the popularity and sales market of Ankang selenium-rich tea are limited. In view of the problems in the marketing of Ankang selenium-rich tea camp, through investigation and empirical analysis, this paper puts forward strategies such as celebrity benefit promotion, tourism commodity promotion, cultural tea brand promotion, festival promotion, tea garden picking promotion and other strategies to promote the marketing of Ankang selenium-rich tea.

The effect of digital capital on the income stability of farmers under the background of COVID-19 epidemic：Mediation effect bassed on the individual dynamic capability

ZHAO Jing, XU Xiu-mei

2021, 60(19): 153-161. doi:10.14088/j.cnki.issn0439-8114.2021.19.033

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Based on the survey data of 212 farmers in Qingdao city, Shandong province, this paper used factor analysis method to measure the farmers' digital capital and their individual dynamic capability, then adopted the individual dynamic capacity as a mediation variable to verify the influence of farmers' digital capital on the stability of their income. The results show that farmers' digital capital can directly and significantly affect the stability of income. The individual dynamic capacity takes a part of mediating effect between farmers' digital capital and the stability of their income, which indirectly affects the stability of farmers' income. Therefore, this paper puts forward policy suggestions from the aspects of improving digital access and digital capability, including improving farmers' digital access from hardware construction and policy design,taking advantage of the digital transformation of agriculture and rural to enhance farmers' digital capability. In order to better enhance farmers' digital capital in digital economy era, and promote the development of digital rural and the steady growth of farmers' income.

Explore diversified compensation methods for mining rights withdrawal in nature reserves：Practical inspiration based on some typical regions

ZHANG Zhe-cong, LI Xiao-yu, LIU Xiao-long

2021, 60(19): 162-167. doi:10.14088/j.cnki.issn0439-8114.2021.19.034

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It is an important measure to explore diversified compensation modes for mining rights withdrawal to comply with the withdrawal trend and improve the exit efficiency. Value evaluation is the precondition of mining right withdrawal compensation. Based on the introduction of cost accounting and value measurement, combined with the practice of the withdrawal of mining rights in nature reserves in China, this paper studied the cash compensation mode of Chongqing, the enterprise transformation mode of Qinghai Western Mining Company and the displacement mode of Guizhou Yongji Mining Company, and analyzed the advantages and disadvantages of each mode. Finally, the specific path to improve the diversification exit compensation was proposed.

Research on influencing factors of land scale management of large grain farmers： Based on survey data of large grain growers in Pingyuan county,Dezhou city

FAN Ling-hui, WANG Mei-xi, HAN Dun-bin, LI Xiao, MU Shao-yan

2021, 60(19): 168-173. doi:10.14088/j.cnki.issn0439-8114.2021.19.035

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Based on the data of 244 large grain growers in Pingyuan county of Dezhou city, 18 variables in the four aspects of personal characteristics, family characteristics, farmers' planting costs and benefits and price of agricultural production means were chosen. Multivariable linear regression model was used to analyze the affecting factors of large grain growers' land management scale. The results show that the educational level, amounts of family labor force, the prices of land transfer, household income, cost per hectare, whether it is easy to hire workers, whether it is easy to lease machinery and whether it is easy to loan and the method of land lease （lease period） are significantly positively correlated with land scale management. The age of large grain growers, whether they have purchased large-scale agricultural machinery, the quality of agricultural materials, and the price of agricultural materials are significantly negative correlation with scale land management.

Research on elements distribution of popular science tourism evaluation system based on IPA method

SHEN Hong-jie, ZHAO Lin-long

2021, 60(19): 174-178. doi:10.14088/j.cnki.issn0439-8114.2021.19.036

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This study used IPA method to analyze the importance and satisfaction of 20 popular science tourism evaluation factors in 6 scenic spots. The results showed that personnel quality, landing projects and promotion were the outstanding problems in the construction of popular science tourism. Finally, according to the distribution of elements in the four quadrants, the development suggestions of key construction projects, timely updating projects and future expansion projects were put forward.

Comprehensive evaluation of the development power of natural villages and discussion on the layout of central villages

TAO Feng

2021, 60(19): 179-183. doi:10.14088/j.cnki.issn0439-8114.2021.19.037

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It is of great significance to carry out village status analysis and development potential evaluation at the level of natural villages, and provide development suggestions and strategies with the regional situation for natural villages with different status conditions, so as to study the internal differences of villages, development needs and the establishment of regional overall development system. From the perspective of development capacity conditions-restrictive factors, by selecting data indicators related to production, life and ecological functions, the comprehensive score of development capacity of each natural villages and the influence degree of influencing factors were calculated, the priority areas of administrative villages were determined, and the development center villages within the ownership range were selected through regional factors.

Research on the coordination degree between population urbanization and basic public service level in Hubei province

LUO Hui, WAN Min

2021, 60(19): 184-189. doi:10.14088/j.cnki.issn0439-8114.2021.19.038

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The coordinated development of population urbanization and basic public service level affects the transformation of China's new urbanization. By establishing the evaluation index system of the two, using the coupling coordination model and spatial autocorrelation methods, this study evaluated the spatial characteristics of the coordinated development of population urbanization and basic public service level in 12 prefecture level cities of Hubei province, and analyzed the influence of economic level and government policy on the coordinated development of the two.

Research on the influence of public service motivation of grass-roots civil servants in Urumqi city on work performance

DUAN Wen-zhi, GUAN Zhi-qiang

2021, 60(19): 190-195. doi:10.14088/j.cnki.issn0439-8114.2021.19.039

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Public service motivation is the guide for promoting individuals to act for the purpose of public interest. In order to further explore the mechanism for improving the level of public service motivation of grass-roots civil servants, 353 grass-roots civil servants in Urumqi city were selected as the research object, and the impact of public service motivation on job performance was studied through regression analysis of data. The results show that public service motivation is common in Urumqi grass-roots civil servants, and public service motivation is at a high level. The attration of public participation and self sacrifice have a positive impact on the work performance of grass-roots civil servants in Urumqi, and put forward relevant suggestions to improve the performance of Urumqi grass-roots civil servants by improving the level of public service motivation.

The supply dilemma and optimization strategy of rural mutual pension service from the perspective of symbiosis theory：Take “H village elderly care home” as an example

GUO Jian-ping, WANG Cai-ling

2021, 60(19): 196-199. doi:10.14088/j.cnki.issn0439-8114.2021.19.040

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As a supplement of family and social pension service, rural mutual pension service aims to fully tap elderly human resources of rural elderly, build a mutual pension model, and provide new ideas for solving problems of empty nest and aging pension in rural areas. In recent ten years, the elderly care home in H village has fall into the development dilemma because of the weak supply body, parasitic supply mode and unfavorable external environment. This paper used the symbiosis theory to analyze the difficulties and reasons of elderly care home mutual aid service supply with the symbiotic unit, symbiotic mode and symbiotic environment. It put forward strategies to effectively promote the supply of mutual pension services from the aspects of establishing a symbiotic unit of shared responsibility, constructing a symbiotic model of symmetrical and mutually beneficial supply, and creating a mutually beneficial symbiotic development environment, in order to solve the problem of rural pension.

Spatial distribution and influencing factors of characteristic towns in Shandong province

DONG Hao, XU Xiu-mei

2021, 60(19): 200-206. doi:10.14088/j.cnki.issn0439-8114.2021.19.041

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125 Characteristic towns published in the national and provincial lists are selected as the research samples, and the spatial distribution characteristics and influencing factors of characteristic towns in Shandong province are analyzed by using ArcGIS 10.2 software. The results show that the spatial distribution of characteristic towns in Shandong province is discrete. There are some differences between the characteristic towns and the uniform distribution in Shandong province,which are in a decentralized state, but the dispersion tends to be uniform. It is roughly divided by the eastern coastline of Dongying and the eastern boundary of Linyi. The number of characteristic towns in the west is large and scattered, while the distribution of cities in the east is relatively dense. The spatial distribution of characteristic towns in Shandong province has a high degree of coordination with economic development and population. The spatial connection is relatively close. The above analysis provides a useful reference for optimizing the spatial layout of characteristic towns in Shandong province.

Research on the blocking mechanism of poverty relief population returning to poverty from the perspective of feasible capability：Taking J city of H province as an example

ZHAO Yu, XIA Qing-li, TIAN Tian

2021, 60(19): 207-211. doi:10.14088/j.cnki.issn0439-8114.2021.19.042

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China's poverty alleviation has achieved a comprehensive victory,but poverty alleviation is not the end. It is necessary to establish an early warning and blocking mechanism for poverty. In view of this, based on the perspective of feasible capability theory, this paper summarized the main methods and results of targeted poverty alleviation in J city,H province, and analyzed the risk points affecting the return of poverty-stricken people in J city, and established a mechanism for preventing the return of poverty-stricken people： Increasing poverty the political participation of the population provides equal social status for the poor, strengthening infrastructure construction and industrial development, and improving the social security system. In order to provide references for consolidating the achievements of poverty alleviation and fostering long-term poverty alleviation mechanisms in poor areas.

Analysis on the path to promote rural revitalization in the process of constructing the new development pattern

GUO Xiao-ping, ZHANG Wen-chang

2021, 60(19): 212-216. doi:10.14088/j.cnki.issn0439-8114.2021.19.043

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This paper mainly discussed how to comprehensively promote rural revitalization in the process of actively constructing a new development pattern, deeply analyzed the era background of China's comprehensive promotion of rural revitalization, and deeply analyzed the important significance of comprehensively promoting rural revitalization in the process of constructing a new development pattern, and explored new paths and new measures to comprehensively promote rural revitalization in the process of building a new development pattern. It is found that the comprehensive promotion of rural revitalization must take multiple measures, and put forward to improve the system and mechanism, strengthen the leadership of party building; Adhere to the “four not picking”, consolidate and expand the results of poverty alleviation; Deepen rural reforms, activate rural elements; Implement rural construction actions to consolidate the foundation for rural revitalization; Activate the urban and rural domestic demand market and construct a new pattern of rural development.

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