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Table of Content

    25 August 2023, Volume 62 Issue 8
    Breeding & Cultivation
    Effects of different nitrogen application rates on the expression of wheat characters based on DUS testing
    SUN Jian-jun, DOU Dan-dan, SHEN Pei-lin, DING Chao-ming, GUO Yu-xi, GUO Xin-hai, WANG De-xin
    2023, 62(8):  1-6.  doi:10.14088/j.cnki.issn0439-8114.2023.08.001
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    To explore the effects of different fertility levels on the expression of wheat (Triticum aestivum L.) traits, so as to provide reference for wheat DUS test, using 4 common wheat varieties as materials, the expression of 32 characters in DUS test was analyzed by setting 5 nitrogen application treatments. The results showed that different nitrogen application levels had a great influence on the expression of awn length, showing two code differences, while there was no difference or one code difference in the expression of quality traits, false quality traits and other visual quantitative traits. The results of variance analysis showed that the effect of nitrogen application on different measurement type traits among different varieties was different, and each trait showed significant differences under different fertility(P<0.05). Among them, the flag leaf length, flag leaf width and spike length were greatly affected by the amount of nitrogen fertilizer, the expression of plant height, spikelet number and grain number per spike were less affected, and the expression of sterile spikelet number was basically not affected.
    Effects of dense planting on growth, agronomic characters and yield of rapeseed
    XIE Xiong-ze, XIE Jie, CHU Qian-mei, YIN Yu-feng, CHEN Fu-hua, FENG Peng
    2023, 62(8):  7-12.  doi:10.14088/j.cnki.issn0439-8114.2023.08.002
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    Rapeseed(Brassica napus L.) inbred lines “20ZY0592”(V1), “20ZY9389”(V2), hybrid variety “Huayouza 62”(V3) and “Huayouza 9”(V4) were used as test materials. A split-plot experiment with three densities of 3×105 plants/hm2(D1), 6×105 plants/hm2(D2), and 9×105 plants/hm2(D3) was set up. The index of extinction, number of green leaves per plant, leaf area index, photosynthetic rate, agronomic characters and yield were measured at the overwintering period, budding period, full flowering period and silique period. The results showed that during the whole growth period of rapeseed, dense planting resulted in the increase of extinction index and leaf area index, the decrease of the number of green leaves per plant and photosynthetic rate. Under different density conditions, the extinction index and leaf area index, the number of green leaves per plant and photosynthetic rate were grouped in pairs, with positive correlation within groups and negative correlation between groups. Dense planting led to the decrease of root-stem diameter, effective branch number, effective length of main inflorescence, effective silique number per plant and the increase of effective branch position. Dense planting was beneficial to increase yield within a certain range, but led to decreased yield beyond the range. Reasonable dense planting was beneficial to increase the yield of rapeseed, but while dense planting increased the leaf area index, it would also lead to the increase of the extinction index, the decrease of the number of green leaves and photosynthetic rate of a single plant, and caused the individual plant to be thinner and center of gravity to rise, thereby increasing the risk of lodging, which needed to be paid attention to in the process of dense planting of rapeseed.
    Resource & Environment
    Analysis of endophytic fungi and the rhizosphere soil fungi communities of Polygonatum cyrtonema Hua.
    LI Ming-jian, CHEN Xiao-fang, CHENG Zhong-jun, REN Xiu-xiu, ZHANG Xiang-yu
    2023, 62(8):  13-20.  doi:10.14088/j.cnki.issn0439-8114.2023.08.00
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    The community composition and diversity of endophytic fungi and rhizosphere soil fungi of wild Polygonatum cyrtonema Hua. from Hunan Province, Guizhou Province and Guangxi Zhuang Autonomous Region was analyzed based on Illumina MiSeq high-throughput sequencing technology. A total of 3 188 ASVs were obtained in all samples, and were divided into 11 phyla, 33 classes, 85 orders, 166 families, 320 genera and 375 species. Fungi mainly belonged to Ascomycota and Basidiomycota. At the genus level, the dominant genera of rhizosphere fungi were Cladophialophora and Exophiala in Guizhou Province, Archaeorhizomyces and Mortierella in Hunan Province, and Fusarium and Saitozyma in Guangxi Zhuang Autonomous Region; the dominant genera of endophytic fungi were Exosporium, Entoloma, etc. in Guizhou Province, while they were Geastrum, Mortierella, etc. in Hunan Province, and they were Fusarium, Saitozyma, etc. in Guangxi Zhuang Autonomous Region. The alpha diversity analysis showed that the diversity and richness of endophytic fungi and rhizosphere soil fungi communities in the medicinal parts of Polygonatum cyrtonema Hua. from these three provinces(region) were significantly different. The results of PCoA and UPGMA clustering showed that the composition of fungal communities in three areas was quite different; PICRUSt 2 predictive analytics showed that endophytic fungi and rhizosphere soil fungi were involved in multiple metabolic pathways and the production of active substances such as polysaccharides of Polygonatum cyrtonema Hua.
    Effects of optimized fertilization on yield, quality and nutrient use efficiency of alpine radish-cabbage rotation system in western Hubei Province
    ZHOU Jian-xiong, TIAN Wei, ZHAO Shu-jun, XU Da-bing, HAN Wei, CHEN Xi-gui
    2023, 62(8):  21-26.  doi:10.14088/j.cnki.issn0439-8114.2023.08.004
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    Different fertilization treatments were set up to study the effects of different fertilization modes on the yield, quality and nutrient utilization rate of the radish (Raphanus sativus L.)-cabbage (Brasscia oleracea L.) rotation system in the alpine region of western Hubei Province with conventional fertilization as control. The results showed that compared with the conventional fertilization control, the yield of radish increased by 11.09% and 38.59% in the treatment of optimized fertilization and organic fertilizer replacing 30% of chemical fertilizer nitrogen, respectively, and in addition to the recovery efficiency of potassium in the optimized fertilization treatment, the recovery efficiency, agronomic utilization rate, partial productivity and harvest index of nitrogen, phosphorus and potassium in the two treatments increased to varying degrees. Compared with the conventional fertilization control, the application amount of phosphate fertilizer and potassium fertilizer in the optimized fertilization model decreased by 50.00% and 16.67%, respectively, but the yield of cabbage decreased by only 1.68%, and except for the decrease of nitrogen agronomic efficiency and partial factor productivity, the nutrient utilization efficiency indicators and harvest index of other nitrogen, phosphorus and potassium all increased. Compared with conventional fertilization, under the treatment of organic fertilizer replacing 30% of chemical fertilizer nitrogen, the yield of cabbage increased by 4.49%, the recovery and utilization rate, agronomic utilization rate and partial productivity of nitrogen and potassium, the agronomic utilization rate and partial productivity of phosphorus all increased. Therefore, the mode of optimized fertilization and the mode of organic fertilizer replacing 30% chemical fertilizer nitrogen had a significant effect on improving the yield and nutrient use efficiency of the alpine radish-cabbage rotation system in western Hubei Province.
    Research on the prediction model of rice yield component factors based on atmospheric circulation characteristics and sea temperature:Taking Jiangsu Province as an example
    HAO Ling, YANG Ying, ZHANG Pei, QIU Hang, QIAN Shen-yang
    2023, 62(8):  27-36.  doi:10.14088/j.cnki.issn0439-8114.2023.08.005
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    Based on the characteristic of the 500 hPa atmospheric circulation characteristic that could characterize the weather situation and control the weather conditions, and the principle of air-sea interaction, the two techniques of linear and monotonic curve correlation and optimization factor correlation were used to conduct census and comparative analysis on circulation and sea temperature factors. A batch of factors of atmospheric circulation characteristics and sea temperature that were extremely significantly related to the yield component factors (effective panicle number, grain number per panicle and thousand-grain weight) of rice (Oryza sativa L.) in Jiangsu Province were selected. Then, the stability of the factors obtained from the general survey was tested by the sliding correlation test, and the independence of the factors obtained from the general survey was tested by the principal component identification method. The factors of atmospheric circulation characteristics and sea temperature that were stable, independent and significantly related to the rice yield component factors were determined as the forecasting factors. Finally, based on the least squares method, circulation and sea temperature models for dynamic prediction of rice yield component factors in Jiangsu Province were established, the fitting effects of these models were good and could be put into business use.
    Study on land ecological security in Zhangjiajie City based on ecological footprint
    HE Bi-hu, DENG Ming-rong, XIE Wen-hai, ZHU Shen-hai, HUANG Cheng-hui, ZENG Sheng, LI Ya-hui
    2023, 62(8):  37-43.  doi:10.14088/j.cnki.issn0439-8114.2023.08.006
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    In order to explore the ecological security and spatial pattern of various types of land in Zhangjiajie City, Hunan Province, based on the ecological footprint analysis, the land ecological footprint, ecological carrying capacity, ecological profit and loss, and ecological pressure index of Zhangjiajie City in five periods of 2000, 2005, 2010, 2015 and 2020 were calculated. Through the establishment of the land ecological security evaluation model, the grade of land ecological security in Zhangjiajie City was evaluated according to the standardized ecological pressure index. The results showed that the ecological footprint per capita of Zhangjiajie City showed an upward trend from 2000 to 2020, with a growth rate of 52.4%; the ecological carrying capacity per capita remained basically stable; the ecological deficit had increased by 1.45 times; the ecological pressure index also increased by 52.2%. The land ecological security in Zhangjiajie City fluctuated, and was generally in an unsafe state. The spatial pattern of land ecological security showed that the unsafe area overlapped with the grassland and water area, and the safe area overlapped with the forest land.
    Spatial autocorrelation analysis study of land use and ecosystem service value in karst area: A case of Baise City in Guangxi
    HUANG Xiu-yu, YAN Zhi-qiang, HU Bao-qing, YAN Qin-qiang, PENG Ding-xin, SHENG Kai
    2023, 62(8):  44-53.  doi:10.14088/j.cnki.issn0439-8114.2023.08.007
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    The spatial distribution pattern of land use and ecosystem service values in Baise City, Guangxi in 2020 was visualized under a 3 km×3 km grid, and their spatial aggregation characteristics and spatial autocorrelation were explored using Moran’s I index and LISA agglomeration map. The results showed that, the various land use types in Baise City showed a significant positive correlation and agglomeration distribution, and the spatial agglomeration or anomalous areas were obviously different. The ecosystem service values of Baise City as a whole showed a significant positive spatial autocorrelation and high-high aggregation, and low-low aggregation,with a high degree of spatial aggregation. High-low aggregation and low-high aggregation appeared sporadically in local areas. Forest land and its ecosystem service values both showed the strongest positive spatial autocorrelation and spatial agglomeration, the positive spatial autocorrelation and spatial agglomeration of other land use types and their ecosystem service values showed the same trend. Baise City should continue to implement ecological protection and restoration policies, strictly prohibit urban construction from touching the "red line" of ecological protection, improve land use efficiency and ecosystem service value in general, and promote regional economic, social and ecologically sustainable development.
    Ecological sensitivity analysis of Zhengzhou City based on FAHP method
    WANG Ya-nan, TANG Pu-xia, WANG Dong-meng, LEI Ya-kai, KONG De-zheng
    2023, 62(8):  54-59.  doi:10.14088/j.cnki.issn0439-8114.2023.08.008
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    Using GIS software, ecological sensitivity analysis was conducted in Zhengzhou City, and ecological sensitivity zoning was carried out. Six factors were selected, including vegetation coverage, elevation, slope, water buffer zone, population density, and land use type,the fuzzy analytic hierarchy process (FAHP) was used to build the evaluation model, and the spatial analysis function of GIS software was used to analyze the ecological sensitivity of a single factor and the comprehensive ecological sensitivity of weighted superposition. The results indicated that Zhengzhou City was mainly composed of moderately sensitive areas, followed by highly sensitive areas; moderately sensitive areas accounted for 37.05% of the research area total area, mainly distributed in Yellow River Basin in the north of Zhengzhou, the plain in the east of city, and the surrounding areas of Songshan Mountains in the west of Zhengzhou City;highly sensitive areas accounted for 29.19% of the research area total area, mainly distributed in low mountain and hilly areas with higher elevations and larger slopes in Songshan and Jishan Mountains;low sensitivity areas accounted for 21.03% of the research area total area, mainly distributed in the Yellow River water area in the north of Zhengzhou City and the surrounding areas of the urban central planning area in the eastern part of Zhengzhou City;the insensitive areas accounted for 12.73% of the research area total area, mainly distributed in the central area in the east of Zhengzhou, the urban built-up areas of Zhongmu County and Xinzheng City under its jurisdiction, and scattered in some parts of the west.
    Integrated analysis of the effect of nitrogen addition on soil organic phosphorus transformation
    WANG Yan-jie, WU Lin-hui, GENG Bi-miao, ZHAO Qiong, SUN Qing-ye
    2023, 62(8):  60-68.  doi:10.14088/j.cnki.issn0439-8114.2023.08.009
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    In order to investigate the impact of nitrogen deposition on soil organic phosphorus mineralization and microbial phosphorus fixation, this study collected 86 pieces of literature on the impact of soil organic phosphorus transformation worldwide and conducted a meta-analysis of 874 sets of data obtained. Quantitative analysis was conducted on the effects of nitrogen addition on soil organic phosphorus transformation related indicators (phosphatase, microbial biomass phosphorus, etc.) and their differences in different types of ecosystems, soil pH, soil depth, nitrogen source, nitrogen addition amount, and duration. Globally, nitrogen addition significantly increased soil acid phosphatase and alkaline phosphatase activities by 10.7% and 8.8%, respectively, and significantly decreased soil microbial biomass phosphorus content by 13.2%. The addition of nitrogen promoted the enhancement of soil organic phosphorus mineralization and inhibited the fixation of phosphorus by microbial biomass. Nitrogen addition reduced soil pH by 4.1%. Soil pH was significantly negatively correlated with acid phosphatase activity, but significantly positively correlated with microbial biomass phosphorus content. Nitrogen addition significantly increased soil phosphatase activity, indicating that nitrogen addition promoted soil organic phosphorus mineralization. The addition of nitrogen significantly reduced the phosphorus content of soil microbial biomass and inhibited the fixation of phosphorus by soil microorganisms. The response of organic phosphorus mineralization and microbial fixation to nitrogen addition varied among different ecosystem types, soil pH, soil depth, and experimental operations. In addition, the change in soil pH caused by nitrogen addition was an important reason for its impact on soil organic phosphorus transformation.
    Value accounting of cultivated land ecosystem products in Karst Plateau areas——Taking Anshun City, Guizhou Province as an example
    ZHOU Fu-kai, WANG Hai
    2023, 62(8):  69-74.  doi:10.14088/j.cnki.issn0439-8114.2023.08.010
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    Taking Anshun City, Guizhou Province as an example, this paper discussed the connotation of the value of cultivated land ecosystem products in Karst Plateau areas, and used mathematical statistics and econometric models to calculate the value by referring to theoretical models and combining the relevant data of cultivated land ecosystem in Anshun City. The results showed that the value of cultivated land ecosystem products in Anshun City in 2019 was 29.235 billion yuan, accounting for 31.64% of the total GDP of Anshun City in 2019; the output value of cultivated land ecosystem products was 16.653 billion yuan, accounting for 56.96% of the value of cultivated land ecosystem products; the regulation services of cultivated land ecosystem was worth 11.166 billion yuan, accounting for 38.20% of the value of cultivated land ecosystem products; the cultural service function of cultivated land ecosystem was worth 1.416 billion yuan, accounting for 4.84% of the value of cultivated land ecosystem products. The research results provided reference for relevant government departments to formulate policies for the protection of the quantity, quality, and ecology of arable land, as well as for the accounting of cultivated land value.
    Plant Protection
    Effects of film mulching on weed control and yield in rapeseed under low level fertilization
    JIANG Cheng-hong, ZHOU Yuan-wei, LI Shuang-hua, LI Xu-qing, SHI Chang-hua, PAN Long-qi
    2023, 62(8):  75-80.  doi:10.14088/j.cnki.issn0439-8114.2023.08.011
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    The effects of film mulching on weed control and yield in rapeseed under low-level fertilization were studied by setting three treatments: Biodegradable film (BE film), polyethylene film (PE film) and no film mulching(control). The results showed that under low level fertilization, both plant height and leaf number of rapeseed seedlings showed a serious deficiency of fertilizer. The correlation coefficient between lack of fertilizer state and N was above 0.95. Film mulching could effectively accelerate rape biomass accumulation. 45 days after emergence, compared to the control, plant height and leaf number of BE film and PE film were increased by an average of 3.8%, 5.4% and 6.1%, 8.1%, respectively, while 75 days after emergence, plant height and leaf number were increased by an average of 17.6%, 14.3% and 20.6%, 14.3%, respectively; film mulching could effectively control weeds. 45 days after emergence, the average plant control efficiency of BE film and PE film was 83.6% and 82.7%, respectively, while 75 days after emergence, the average plant control efficiency was 89.1% and 84.7%, and the average fresh weight control efficiency was 73.4% and 85.4%, respectively; at the same time, film mulching could also change the community structure of dominant weeds. Compared to the control, covering BE film and PE film, the average height of rapeseed plant increased by 5.6% and 5.0%, respectively, the number of primary effective branches increased by 13.2% and 22.6%, respectively, the effective pod number per plant increased by 20.6% and 22.5%, respectively, the seed number per pod increased by 30.1% and 27.5%, respectively, and the average yield increased by 55.2% and 47.5%, respectively.
    Fungicidal effect of plasma on Penicillium of lily
    LIU Cui-cui, GAO Si-fan, WU Shen-zhong, NING Guo-gui, HE Yan-hong
    2023, 62(8):  81-84.  doi:10.14088/j.cnki.issn0439-8114.2023.08.012
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    Bulb scales of Lilium oriental hybrids Sorbonne inoculated with Penicillium were treated with ozone and nitrogen oxides generated by the plasma device to explore the bactericidal effect of plasma on Penicillium of lily and the effect of ozone treatment on plant growth and development. The results showed that both plasma gases treated for 20 min every two days or four days effectively inhibited Penicillium of lily, and the incidence rate could be controlled to 11.11%~22.22%. The ozone generated by plasma could effectively inhibit the occurrence of Penicillium without affecting the growth and development of lily. This study was the first report of plasma technology in the control of Penicillium in lily bulbs, which was beneficial to promote the domestic localization of lily bulbs and green ecological agriculture.
    Horticulture & Local Products
    Optimization of Ganoderma lucidum fermentation process with lotus leaf substrate and determination of active components
    YANG Cong-fa, TANG Ben, QIN Qi, LUAN Li-ning, ZHU Yong-quan, DENG Xiang, LI Xiang
    2023, 62(8):  85-89.  doi:10.14088/j.cnki.issn0439-8114.2023.08.013
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    Ganoderma lucidum fermentation process with lotus leaf as the main substrate was studied and the content of active components before and after fermentation was determined. Biomass was selected as the detection index, and the effects of water material ratio, carbon source(glucose), nitrogen source(soybean protein) and lotus leaf specification on Ganoderma lucidum fermentation were studied by single factor test and orthogonal test. The results showed that the optimal culture conditions of lotus leaf fermented by Ganoderma lucidum were as follows: water material ratio of 3.0∶1, carbon source(glucose) 10%, nitrogen source(soybean protein) 4%, magnesium sulfate heptahydrate 0.1 g, potassium dihydrogen phosphate 0.15 g, lotus leaf specification 40 mesh particle size, fermentation temperature 28 ℃, and fermentation time 12 d. After fermentation, the contents of active components in the substrate were alkaloid 0.508%, polysaccharide 4.870%, ganoderma acid 0.720%, soluble dietary fiber 3.450% and insoluble dietary fiber 9.020%, which was a good raw material for a functional food.
    The effect of root promoting fertilizer on seedlings growth of Bupleurum chinense DC.
    LI Xiao, WANG Dan, LI Yi-xue, CHEN Hua, YU Ma, ZHAO Jun
    2023, 62(8):  90-94.  doi:10.14088/j.cnki.issn0439-8114.2023.08.014
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    In order to increase the yield of the underground part of Bupleurum chinense DC., nine types of root promoting fertilizers were used to irrigate and fertilize B. chinense seedlings. The effects of different root promoting fertilizers on eighteen agronomic traits such as B. chinense plant height, root dry weight, and chlorophyll content, as well as saikosaponins content and yield were studied. The results showed that Micro Tonic Energy increased the aboveground dry weight; Genduole inhibited the growth trend of plant height and delayed the attenuation and growth trend of chlorophyll content; Haibos Growth Promotion Essence increased the plant height, but inhibited the growth trend of plant height; among them, five fertilizers reduced the yield of saikosaponins. In the production and cultivation of B. chinense, it was extremely important to apply fertilizer reasonably to inhibit the growth of aboveground organs, promote root growth, and facilitate biosynthesis and accumulation of saikosaponins.
    Effects of melatonin pretreatment on drought resistance of strawberry seedlings
    GOU Yi-jie, YANG Zhen-hua
    2023, 62(8):  95-101.  doi:10.14088/j.cnki.issn0439-8114.2023.08.015
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    In order to study the effects of melatonin pretreatment on drought resistance of strawberry seedlings, strawberry seedlings were treated with different concentrations of melatonin in “spray+root irrigation” pre-culture before drought treatment. The results showed that drought severely inhibited strawberry growth. Medium and low concentration(100~200 μmol/L) melatonin pretreatment could effectively alleviate the growth stress caused by drought on strawberry seedlings, enhance the activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT), and inhibit the production of hydrogen peroxide (H2O2) and malondialdehyde(MDA). Meanwhile, drought stress increased the expression levels of stress-related genes in strawberry seedlings, and exogenous melatonin pretreatment could further enhance the expression levels and significantly affect the expression of key enzyme genes of the melatonin synthesis pathway, so as to improve the drought resistance of the strawberry.
    Study on the adaptability of flue-cured tobacco variety Yunyan 87 to the intensity of threshing and redrying
    HE Bin, CAI Bin, HU Yong
    2023, 62(8):  102-106.  doi:10.14088/j.cnki.issn0439-8114.2023.08.016
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    In order to explore the adaptability of flue-cured tobacco variety Yunyan 87 to threshing intensity and redrying intensity, the experiment was conducted with the middle C3F and upper B2F tobacco leaves of Yunyan 87 as materials, and five threshing intensities and five redrying intensities were set to study their effects on the percentage of large and medium slices, stalk content and sensory quality of tobacco leaves after threshing and redrying. The results showed that with the increasing of threshing intensity, the stalk-containing rate of the middle leaves showed an overall upward trend, and the upper-leaf stalk rate showed an overall trend of first increasing and then decreasing. The highest and lowest stem content of middle leaves were 1.53% and 1.43% respectively, and the highest and lowest stem content of upper leaves were 1.06% and 0.83% respectively; the content of thick stalks in the middle and upper leaves did not change much. With the increasing of threshing intensity in the middle leaves, the ratio of large pieces, fragments and broken pieces decreased, the rate of medium and small pieces showed an overall increasing trend, and the rate of large and medium pieces did not change much. With the increasing of the threshing intensity of the upper leaves, the large and medium flake rates first increased and then decreased, and the medium flake rate, the small flake rate, the fragment rate and the powder rate showed a trend of first decreasing and then increasing. With the decreasing of the re-baking intensity, the strength of the middle leaves was the highest at the intensity 2; the transparency and the thick feeling showed a decreasing trend; the smoothness, longness, softness and roundness were all the best at the intensity 3. The strength, transparency and thickness of the upper leaves decreased with the reduction of the redrying intensity; the smoothness, longness, softness and roundness were all the best at intensity 3. To sum up, high-strength threshing could effectively reduce the rate of large leaves in the middle leaves, increase the rate of medium leaves, reduce the rate of fragments and powders, and help control the structural indicators of tobacco leaves; the intensity of 2 could satisfy standard requirements for both the large and medium rates of upper leaves. Redrying intensity 3 had the smallest loss of aroma, which was beneficial to reduce miscellaneous air, and the overall sensory quality was better.
    Relationship between transplanting period and yield and quality of fresh and sweet flavor type tobacco of Baoshan City
    JI Shun-hua, CHEN Yi-qiang, HUANG Jian-bin, DENG Xiao-hua, LIN Xian-ta, CUI Zhen-wei, LAI Rong-hua, YE Run, ZHANG Yong-an, QI Ling-feng
    2023, 62(8):  107-112.  doi:10.14088/j.cnki.issn0439-8114.2023.08.017
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    In order to study the relationship between transplanting period and yield and quality of sweet flavor style tobacco, an experiment on the relationship between yield and quality of Yunyan 116 variety and different transplanting periods was carried out in Baoshan City, Yunnan Province from 2018 to 2020. The field growth, economic traits and field diseases of tobacco plants were compared, and the appearance quality, sensory quality and conventional chemical composition of test samples were compared and analyzed. The results showed that early transplanting could improve the sensory style characteristics, aroma characteristics, smoke characteristics, and taste characteristics of fresh and sweet flavor type tobacco of Baoshan City. However, if transplanted too early or too late, there would be a significant increase in field diseases, a decrease in the growth of tobacco plants in the field, and a decrease in economic benefits, and the sensory quality significantly decreased after transplanting too late. Considering the comprehensive yield and quality, it was advisable to transplant from April 27th to May 4th in the tobacco district of Baoshan City.
    Effects of arbuscular mycorrhizal fungi on growth and selenium accumulation of peach seedlings
    SHU Jun-jiang, BAO Rong-fen, HUANG Ke-wen, LIU Lei, HUANG Yan, LIN Li-jin, WANG Ting
    2023, 62(8):  113-119.  doi:10.14088/j.cnki.issn0439-8114.2023.08.018
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    The effects of four arbuscular mycorrhizal fungi Glomus mosseae, Acaulospora delicata, Paraglomus occultum and Glomus etunicatum on growth and selenium accumulation of peach [Prunus persica (L.) Batsch] seedlings were studied by a pot experiment. The results showed that application of Acaulospora delicata increased the biomass of peach seedlings, promoted the growth of peach seedlings, and increased the biomass of the root and aboveground parts of peach seedlings by 11.28% and 9.18%, respectively, compared with no application. It also increased the content of photosynthetic pigments, peroxidase activity and catalase activity to a certain extent. Application of Glomus mosseae, Paraglomus occultum and Glomus etunicatum decreased the biomass, photosynthetic pigment content and antioxidant enzyme activity of peach seedlings or had no significant effects on them. Application of Acaulospora delicata also increased the total selenium content and organic selenium content in peach seedlings to a certain extent, and the total selenium content and organic selenium content in shoots increased by 8.01% and 9.34%, respectively, compared with no application. Application of Glomus mosseae, Paraglomus occultum and Glomus etunicatum decreased the total selenium and organic selenium contents in peach seedlings or had no significant effects on them.
    Analysis of the mechanism of walnut therapy for hyperuricemia based on network pharmacology and molecular docking technology
    XU Dong-yue, ZHAO Sheng-lan
    2023, 62(8):  120-126.  doi:10.14088/j.cnki.issn0439-8114.2023.08.019
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    Based on network pharmacology and molecular docking, the mechanism of action of Juglans regia L. in the treatment of Hyperuricemia (HUA) was analyzed, and the Traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) was used to predict the target of active components in walnut. OMIM, GeneCards and DrugBank databases were used to search for HUA disease targets. Cytoscape 3.7.2 software was used for target-active ingredient-disease network mapping, and Metascape platform was used for enrichment analysis. Molecular docking was performed using AutoDockTools 1.5.6 software to verify the binding degree of active ingredients and important targets. The results showed that 5 active compounds of walnut were selected, and 16 common targets of walnut and HUA were identified. The highly enriched signaling pathways include cancer signaling pathway, chemical carcinogenic-receptor activation signaling pathway, advanced glycation endproducts receptor for advanced glycation end products signaling pathway in diabetic complications, and Kaposi sarcoma-associated herpes virus signaling pathway. The molecular docking test showed that the main active compounds in the walnut were closely bound to important targets.
    Analysis of spatial differentiation and influencing factors of key villages in Zhejiang Province: Based on three types of national villages
    CHU Xiang-zhai, YAN Xue-xin, SHENG Fu-bin
    2023, 62(8):  127-132.  doi:10.14088/j.cnki.issn0439-8114.2023.08.020
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    The spatial distribution characteristics and influencing factors of national forest villages, traditional Chinese villages and national key rural tourism villages in Zhejiang Province were analyzed by using mathematical statistics, nearest neighbor index, kernel density analysis and geographic detector, and then the development path of village advantage excavation and diversification was put forward. The results showed that the national forest villages and traditional Chinese villages were concentrated in the middle and south of Zhejiang Province, while the national key rural tourism villages were located in the north and east of Zhejiang Province. Compared with the cultural landscape, the national key rural tourism villages in Zhejiang Province were dominated by natural scenery, and the ecological advantages of the villages were more easily transformed into tourism development advantages. Both national forest villages and Chinese traditional villages were characterized by agglomeration in distribution, and the spatial patterns were agglomeration axial extension and small-scale point-like agglomeration, respectively; the driving factors and influence of the spatial distribution of villages were different. The distribution of national key villages of rural tourism had the strongest correlation with economic factors, and the influence of natural factors on the distribution of national forest villages was more significant. Each factor had a strong explanatory power for the distribution of traditional Chinese villages.
    Aquatic Sciences
    Comparison of anesthetic effect of MS-222 and eugenol on Xenocypris davidi
    CHEN Jun-ping, WU Hui-hui, SHEN Fang-fang, ZHANG Jia-xin, YU Ruo-meng, FU Yong-jie, ZHAO Dao-quan, XIE Guo-qiang, SUN Wen-xi
    2023, 62(8):  133-139.  doi:10.14088/j.cnki.issn0439-8114.2023.08.021
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    In order to study the anesthesia effect of MS-222 and eugenol on Xenocypris davidi, the Xenocypris davidi with a body weight of (20.10±1.28) g was selected and the anesthesia effect of the Xenocypris davidi was observed under different conditions. The results showed that with the increase of the concentrations of the two anesthetics, the time spent in anesthesia for Xenocypris davidi showed a decreasing trend, the recovery time of the MS-222 group was shortened, and the recovery time of eugenol was prolonged. When the mass concentration of MS-222 was 120 mg/L, or the mass concentration of eugenol was 30~60 mg/L, the Xenocypris davidi could be anesthetized within 3 minutes and resuscitated within 5 minutes. With the increase of the concentration of the two anesthetics, the respiratory rate of the experimental fish first increased and then decreased. Under the condition of the same mass concentration of the two anesthetics, with the continuous increase of the water temperature, the anesthesia time of the Xenocypris davidi showed a decreasing trend. And the recovery time of the eugenol treatment group continued to shorten, while the recovery time after MS-222 anesthesia was not significantly different at 10~20 ℃, and was significantly prolonged at 25 ℃(P<0.05). After anesthesia, with the increase of exposure time in the air, the resuscitation time of the MS-222 group showed a continuously prolonged trend, and the resuscitation time of the eugenol group was shortened at 4 minutes, and then continued to prolong. The MS-222 of 120 mg/L and eugenol of 30~60 mg/L had good anesthesia effect on Xenocypris davidi, and both could be used as ideal anesthetics for Xenocypris davidi.
    Effects of environment on the artificial breeding of Charybdis feriatus and the growth of juvenile crab
    ZOU Xiong, YANG Ming-qiu, PU Li-yun, LU Jian-xue, XIA Lian-jun, LIU Xin
    2023, 62(8):  140-143.  doi:10.14088/j.cnki.issn0439-8114.2023.08.022
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    The impact of the current aquaculture environment on the artificial reproduction and juvenile crab growth of Charybdis feriatus was studied, and the breeding density test, bait test and shelter test were conducted. The results showed that, ①the weight of Charybdis feriatus juvenile crab in each stage increased first and then decreased with the increase of breeding density. In the second stage, the weight of D2 group juvenile crab was the largest, indicating that the breeding density of 150 per square meter could ensure the normal development of juvenile crab weight; ②The biological feed feeding group had the lowest body weight of Charybdis feriatus juvenile crab, while the mixed feed feeding group had the highest body weight of Charybdis feriatus juvenile crab. The biological feed feeding group had the lowest shell width and length, and the survival rate of Charybdis feriatus juvenile crab was inversely proportional to the incubation period of the juvenile crab; ③There was a significant difference in the body weight of Charybdis feriatus juvenile crab between different shading test groups. The S1 and S3 groups of Charybdis feriatus juvenile crab had higher body weight values. Under the condition of no shelter, the body weight value of the Charybdis feriatus was the lowest.
    Storage & Processing
    Study on the relationship between shrinkage rate and conventional physical indexes of tobacco based on redundancy analysis
    YANG Yang, YANG Ting, YANG Yu-bo, REN Ming-chao, ZHANG Jin, GONG Shuang, YANG Sheng-gang, WU You-xiang, WANG Jian-min
    2023, 62(8):  144-148.  doi:10.14088/j.cnki.issn0439-8114.2023.08.023
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    In order to explore the relationship between shrinkage rate of tobacco and traditional physical indicators, the leaf density, leaf thickness, equilibrium moisture content, smoldering time, tensile strength and shrinkage rate of 15 kinds of tobacco were measured, and the measurement results were analyzed by means of variance analysis, simple correlation analysis and redundancy analysis. The results showed that the producing area and position of tobacco leaf had significant effects on the shrinkage rate of tobacco, but the position had a greater effect; the shrinkage rate decreased with the decrease of position, and the range of reduction varied with different producing areas. There was a strong correlation between shrinkage rate and leaf density and leaf thickness. In addition, shrinkage rate raised with the increase of leaf density and leaf thickness. When studying the system composed of qualitative variables such as tobacco producing area and position and quantitative variables which were mainly composed of various physical and chemical indicators, redundancy analysis was a suitable method. It could not only analyze the impact of qualitative variables on quantitative variables, but also explore the correlation between quantitative variables based on the relationship between quantitative variables and qualitative variables, so as to understand the relationship between indicators in essence.
    Effects of ultrasonic cleaning on quality of live Patinopecten yessoensis
    LI Xin, PAN Lan-lan, ZHANG Yuan, QU Min, ZHAO Chen-ran, LI Tian-yu, LUAN Xin-tong
    2023, 62(8):  149-154.  doi:10.14088/j.cnki.issn0439-8114.2023.08.024
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    The ultrasonic cleaning tests were carried out at three times (0, 5 and 10 min), the change law of the water index and quality index of Patinopecten yessoensis was analyzed, the best cleaning time of Patinopecten yessoensis was determined, and the circulation and transportation of three groups scallops under modified atmosphere packaging (80%O2,20%N2) were simulated to analyze the effects of different cleaning time on the circulation quality of live Patinopecten yessoensis. The results showed that the survival rate of three groups of Patinopecten yessoensis was 100%, and there was no significant effect on texture and glycogen. Ultrasonic cleaning for 10 min had the highest impurity removal rate of 4.02% and the least total number of colonies of 1.1×105 CFU/mL, water quality got worse after cleaning. Under different cleaning time, the colony number of Patinopecten yessoensis in the simulated transportation process showed an upward trend with the extension of cleaning time. The colony in Patinopecten yessoensis after ultrasonic cleaning for 10 min increased slowly with the extension of transportation time. Ultrasonic cleaning for 10 min was an ideal cleaning time, and the quality of Patinopecten yessoensis remained good.
    Design and process test of microwave cracking equipment
    LUO Wen-jie, ZHANG Jia-xi, LI Hua, LIU Na, WANG Yan
    2023, 62(8):  155-159.  doi:10.14088/j.cnki.issn0439-8114.2023.08.025
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    In order to improve the recycling of agricultural waste, agricultural equipment for the recycling of cracked and carbonized waste crops was designed. The maximum microwave power of the equipment was 3.6 kW, and the frequency was 2.45 GHz, which was commonly used in industry. The diameter of the cracking chamber was 120 mm, and the height was 700 mm. HFSS was used to simulate the electric field in the cracking chamber, and the electric field distribution in the cracking chamber was relatively uniform. The three factors and three levels interactive technological test was designed. Taking cracking chamber diameter size, microwave power and cracking temperature as the test factors, the primary and secondary test factors could be determined as cracking chamber diameter size>microwave power>cracking temperature through the test. Through the Design-Export optimization, the optimal test scheme was microwave cracking chamber diameter of 81.84 mm, microwave power of 1.95 kW, and cracking temperature of 325.51 ℃, and the carbon yield was 30.16%. After test verification, the carbon yield of the cotton stalk was 29.87%, which was slightly different from the software optimization value, so the equipment met the design requirements.
    Study on ultrasonic-assisted extraction process, antioxidant activity and stability of polysaccharides from black quinoa
    YANG Min, XI Jun-wei
    2023, 62(8):  160-166.  doi:10.14088/j.cnki.issn0439-8114.2023.08.026
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    The Response Surface Methodology was used to optimize the extraction process of polysaccharides from black quinoa by ultrasonic-assisted extraction. The antioxidant activity of the polysaccharides from black quinoa was determined by DPPH radical scavenging rate, ABTS+ scavenging rate, total antioxidant capacity and hydroxyl radical scavenging rate. The effects of temperature and pH on the stability of the antioxidant activity of polysaccharides from black quinoa were investigated. The results showed that the optimum extraction condition of the polysaccharides from black quinoa was ultrasonic extraction time of 30 min, ultrasonic extraction temperature of 60 ℃, and solid-liquid ratio of 1∶9 g/mL. The extraction rate of polysaccharides from black quinoa was 9.829 4%. The antioxidant activity of polysaccharides was determined by four methods, it showed that the higher the purity of the polysaccharide, the stronger the antioxidant activity. The stability of the antioxidant activity of the black quinoa polysaccharide changed significantly under the influence of temperature. The higher the temperature, the longer the heating time, and the lower the stability. The antioxidative stability was better under neutral or partial acid conditions. In conclusion, the black quinoa polysaccharide had significant antioxidant activity, and its stability was affected by temperature and pH.
    Research on the optimization of the extraction process and antioxidant activity of total flavonoids from Pileostegia tomentella by response surface methodology
    LI Qiang, ZHOU Ying, LU Sen-hua
    2023, 62(8):  167-174.  doi:10.14088/j.cnki.issn0439-8114.2023.08.027
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    Using a single factor experiment to determine the ultrasonic extraction of total flavonoids from Pileostegia tomentella,three-level Box-Behnken response surface methodology was used to optimize the extraction process with three factors of extraction temperature, ethanol volume fraction, and solid-liquid ratio, and the scavenging rate of DPPH free radicals and reducing ability were calculated. The results showed that the condition for the optimal extraction process of total flavonoids from Pileostegia tomentella was extraction temperature of 49 ℃, ethanol volume fraction of 46%, and solid-liquid ratio of 1∶66 g/mL. The total flavonoids yield from Pileostegia tomentella under the condition was 27.01 mg/g. There were significant differences in the total flavonoids content of Pileostegia tomentella in different origins. In the measured mass concentration, the total flavonoids from Pileostegia tomentella had a good scavenging ability to DPPH free radicals, and its reducing ability increased with the increase of total flavonoids concentration. The extraction process of the total flavonoids from Pileostegia tomentella was stable, the operation was simple, and the methodology was good, which could be used for its large-scale industrial production.
    Influence and application research of ambient temperature and humidity in primary processing on tobacco moisture content between key processes
    TANG Jun, ZHOU Bing, YI Bin, LI Chao, LIN Wen-qiang, CAI Bo, TANG Li, TAN Guo-zhi, HE Bang-hua
    2023, 62(8):  175-181.  doi:10.14088/j.cnki.issn0439-8114.2023.08.028
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    In order to reveal the influence of ambient temperature and humidity on the processing quality, the influence and application of ambient temperature and humidity in primary processing on tobacco moisture content between key processes were systematically studied. The results showed that the indoor ambient temperature of primary processing was significantly higher than the outdoor ambient temperature, and there was a strong positive correlation between them; the closer to the outside, the outdoor ambient temperature and humidity were more affected by the indoor ambient temperature and humidity. Under the conditions of the primary processing, the ambient humidity had a strong negative correlation with the tobacco moisture content between loosening and conditioning process and tobacco casing process, and the correlation coefficient was -0.705 3; the ambient temperature had a strong positive correlation with the tobacco moisture content between tobacco casing process and drying process of cut tobacco, and the correlation coefficient was 0.831 4; the influence of ambient temperature and humidity on the tobacco moisture content between drying process and cooling process of cut tobacco could be ignored. Based on the variation factors of ambient temperature and humidity of the primary processing, the reasonable optimization design of the technical standard of the primary processing could effectively improve the stability of processing quality, which would have important practical significance in improving the stability of cigarette processing quality and product quality.
    Information Engineering
    High resolution image farmland extraction based on RMAU-Net network model
    YUAN Peng, WANG Ke, XIAO Jian
    2023, 62(8):  182-188.  doi:10.14088/j.cnki.issn0439-8114.2023.08.029
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    In order to solve the problem that the traditional full convolutional neural network could not achieve accurate extraction of cultivated land from high-resolution image, this study used the high-resolution 2 remote sensing satellite imagery as the data source, and used the improved U-Net network model (RMAU-Net network model) that integrated residual structure and multiple attention mechanisms to extract the cultivated land in the study area. The RMAU-Net network model was trained by using cultivated land samples, and cultivated land was extracted from the test set images using the trained network model. In order to verify the effect of RMAU-Net network model in extracting cultivated land, three traditional full Convolutional neural network models, DeeplabV3+, PSPNet and U-Net, were selected for comparative analysis with RMAU-Net network model. The results showed that the accuracy, recall, Intersection over Union, and F1 score of the RMAU-Net network model extraction were 90.36%, 90.78%, 82.57%, and 90.57%, respectively. Compared with DeepLabv3+, PSPNet, and U-Net network models, the RMAU-Net network model performed the best. RMAU-Net network model provided new ideas and methods for precise extraction of cultivated land, and provided basic data support for practical applications such as crop area monitoring and yield estimation.
    Risk assessment of urban flood disasters based on multi-source spatial data——Taking Linfen City, Shanxi Province as an example
    XUN Jia-chang, NIE Cong, XU Dong, FANG He
    2023, 62(8):  189-196.  doi:10.14088/j.cnki.issn0439-8114.2023.08.030
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    Based on the theory of natural disaster risk, combined with multi-source spatial data (remote sensing data, reanalysis data, socio-economic data), the risk of disaster causing factors, the sensitivity of disaster-pregnant environment, and the ability to prevent and reduce disasters of flood and waterlogging disasters in Linfen City, Shanxi Province were evaluated. Taking into account the actual situation of Linfen City, 15 evaluation indicators were finally selected, and the weighted comprehensive index method was used for quantitative analysis, a comprehensive risk assessment model for flood disasters was built, and the distribution of flood risks was explored. The results indicated that the risk distribution of disaster causing factors was higher in the eastern and western regions than in the central region, and the overall trend was higher in the eastern region than in the western region; plains and basins with low terrain and small slopes were prone to flood influx, resulting in higher sensitivity to disaster environments. Regions such as mountainous and hilly areas generally had lower sensitivity to disaster environments; high urbanization level and densely populated areas had strong disaster prevention and reduction capabilities, while natural areas such as river valleys and mountains had overall weak disaster prevention and reduction capabilities; the overall distribution of comprehensive risk of flood and waterlogging disasters was gradually increasing from the west to the east, which was reflected in the high risk of river valleys, plains and basins with low economic level, heavy rainfall and large runoff. More than 50% of Linfen City was facing the risk of flood and waterlogging disasters. In general, the flood disaster had a great impact on the social, economic and ecological development of Linfen City.
    Target counting method of grain particle images based on Euler numbers
    KANG Shi-ying, YAO Bin
    2023, 62(8):  197-201.  doi:10.14088/j.cnki.issn0439-8114.2023.08.031
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    In order to solve the problems of time-consuming and low accuracy in manual counting during the process of grain breeding, a method of counting using computer processing of grain particle images was proposed. Firstly, the collected grain particle images was binarized to separate the background and target regions in the image; then morphological operations, object segmentation, and other preprocessing on the image were performed to minimize the phenomenon of grain particle adhesion in the image; in the final counting link, in order to improve the counting speed, based on the important feature that the grain particle images had no holes after preprocessing, the image Euler numbers algorithm was used to replace the traditional connected domain marking algorithm to achieve the counting work.The results showed that the counting result of the image Euler numbers algorithm was completely consistent with that of the traditional connected domain labeling algorithm, but the counting speed of the image Euler numbers algorithm was obviously better than that of the traditional connected domain labeling algorithm.
    Construction and operational strategy of order agricultural system based on blockchain technology——Taking Ningxiang pig in Ningxiang County, Changsha City as an example
    ZHAO Hao-jian, ZHANG Huan-chun, LIU Ning, GUO Dui-tian, LI Shu-ping, ZHANG Li-li, ZOU Xiao-yu, YANG Jian-chao
    2023, 62(8):  202-206.  doi:10.14088/j.cnki.issn0439-8114.2023.08.032
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    Taking the Ningxiang pig order agriculture project in Ningxiang County, Changsha City as an example, this paper introduced the concept, characteristics, advantages, and development process of order agriculture, analyzed the integrity and information opacity issues faced by China’s order agriculture, and proposed the concept of an agricultural industry SaaS service platform based on blockchain technology, as well as the organizational structure and operational strategy of the platform. The actual investment effect of the blockchain order agriculture SaaS platform was showcased, including increasing market volume, reducing costs, increasing revenue, and achieving financing.Blockchain technology could provide data security, traceability, integrity, regulation, and other guarantees for order agriculture, promote the healthy development of order agriculture, and create a new situation of balanced agricultural supply and demand, green and high efficiency, affluent farmers, food safety, and reasonable prices.
    Citrus huanglongbing plant monitoring model based on UAV low altitude hyperspectral remote sensing imaging
    LI Min, QIN Ze-lin, LAN Zong-bao, FANG Hui, YU Sheng-xin, MO Xiao-xiang, XIE Guo-xue, ZENG Zhi-kang
    2023, 62(8):  207-212.  doi:10.14088/j.cnki.issn0439-8114.2023.08.033
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    Taking the citrus (Citrus reticulata Blanco) in Jiujian Village, Pingshan Town, Luzhai County, Liuzhou, Guangxi as the research object, the citrus huanglongbing (HLB) plants were identified through the ground manual measurement, and the hyperspectral imaging of the calibrated citrus planting plot was obtained by cooperating with UAV low altitude remote sensing; the average spectra of the regions of interest (ROI) in the canopy of healthy citrus plants and HLB plants were calculated, and outlier removal, smooth denoising, and spectral transformation on the initial spectra were performed to obtain the original spectra, first-order differential spectra (FDR), and second-order differential spectra (SDR);after dimensionality reduction using principal component analysis, a support vector machine (SVM) classification model was constructed. The results showed that by selecting feature bands ranging from 400 to 1 000 nm and using ArcGIS software to extract sample average spectra, the classification accuracy of the training and testing sets of the full band first order differential spectra reached 87.41% and 84.67%, respectively. The SVM classification model parameters were C=35.39 γ= 0.01;using ENVI software to extract the average spectrum of samples, the classification accuracy of the training and testing sets for the full band first-order differential spectrum reached 92.39% and 96.43%, respectively. The SVM classification model parameters were C=5.06 γ=1.02. UAV low altitude remote sensing and hyperspectral monitoring of citrus HLB was feasible, which could quickly identify HLB plants in citrus plantations.
    Economy & Management
    Research on the effect and promotion strategy of scientific and technological innovation in promoting urbanization construction
    XIE Xin-yue, WANG Lei
    2023, 62(8):  213-222.  doi:10.14088/j.cnki.issn0439-8114.2023.08.034
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    Based on the construction of the evaluation index system of scientific and technological innovation and urbanization level, the entropy weight method was used to calculate the level of scientific and technological innovation and urbanization in Changzhou City from 2009 to 2020, and the driving effect of scientific and technological innovation on urbanization construction was analyzed by the multiple linear regression model. The results showed that the scientific and technological innovation ability and urbanization level in Changzhou City had been greatly improved and were in a stable upward trend. Three indicators of scientific and technological innovation ,namely, the resources allocation of scientific and technological innovation, output of scientific and technological innovation and financial input of scientific and technological innovation all had a positive role in promoting the level of urbanization. Accordingly, some measures of strengthening the transformation of scientific and technological achievements, designing innovation driven strategies according to local conditions, and managing high-level talents in an all-round way were put forward, so as to realize the improvement of the level of scientific and technological innovation in promoting urbanization construction.
    Study on the analysis of the coordinated development trend of provincial and regional economy-ecological environment- tourism industry and obstacle degree
    MIAO Xin-ping, XIE Xue-mei
    2023, 62(8):  223-230.  doi:10.14088/j.cnki.issn0439-8114.2023.08.035
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    Based on the perspective of system science, the temporal and spatial evolution, spatial agglomeration and obstacle factors of the coordinated development of 31 provinces (cities) in China were explored by constructing the evaluation index system of regional economy-ecological environment-tourism industry, and using coupling coordination model, cold and hot spot analysis and obstacle degree model. The results showed that the evaluation value of China’s regional economy, ecological environment and tourism industry was constantly rising, and the tourism industry and regional economic development were fast and had a high correlation. From the perspective of time, the level of coordinated development of all provinces fluctuated and increased, and the coordination level gradually improved, but the development speed of different provinces was quite different; from a spatial point of view, the level of coordinated development was roughly reduced in steps from east to west, except for a few provinces on the eastern coast whose dominant type was regional economy, the rest of the provinces were ecological environment, while the lagging type was roughly manifested as lagging tourism. The hot spots of coordinated development were mainly concentrated in the eastern coastal provinces, and the cold spots were mainly concentrated in the northwest. The cold spots and sub-cold spots were decreasing, and the sub-hotspot areas were increasing in the central provinces. The obstacle degree of regional economy and tourism industry continued to decline slightly, the obstacle degree of ecological environment gradually increased, and the inbound tourism revenue, inbound tourist numbers, regional general budget revenue, urban green space area, and industrial pollution control investment were the five most important obstacle factors.
    Legal risk and system optimization of O2O mode of rural land transfer
    YAN Xia-qiu, HE Si-yi
    2023, 62(8):  231-237.  doi:10.14088/j.cnki.issn0439-8114.2023.08.036
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    The legal risks of O2O mode of land transfer,such as unclear land ownership confirmation, non-standard land transfer procedures and imperfect supervision and evaluation mechanism were analyzed. It was concluded that improving the legal system of O2O mode of land transfer needed to solve the contradiction between the scale of rural land transfer and the agricultural industrial structure, as well as the contradiction between the convenience of rural land transfer and rural environmental protection. It also proposed that under the guidance of the principle of sustainable development, the legal risks of O2O mode of land transfer should be solved by responding to the profit-oriented nature of e-commerce transactions, improving the system of land ownership confirmation, registration and certification, perfecting e-commerce transaction procedures, innovating the third-party supervision and evaluation mechanism, giving full play to the role of the Internet in activating the land transfer market, improving the efficiency of rural land transfer,and promoting agricultural modernization and farmers’ well-being.
    Research on the evaluation and development of the integration level of primary, secondary and tertiary industries in rural areas of Guangdong Province: Based on entropy method and TOPSIS analysis method
    YIN Ya-yue, GAO Peng, DUAN He-rong, BAI Fu-chen
    2023, 62(8):  238-245.  doi:10.14088/j.cnki.issn0439-8114.2023.08.037
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    Based on relevant literature, a comprehensive evaluation index system for the integration of rural primary, secondary and tertiary industries in Guangdong Province was constructed from two dimensions of behavior and performance, and the entropy method and TOPSIS analysis method were adopted to make a comprehensive evaluation of the integration level of rural industries in Guangdong Province. The results showed that the integrated development level of the primary, secondary and tertiary industries in rural areas of Guangdong Province showed a steady upward trend in general, and the comprehensive scores of each secondary subsystem also showed an overall upward trend, and the closeness degree of TOPSIS ideal points increased year by year, which indicated that the significant effect of integration measures of the primary, secondary and tertiary industries in rural areas of Guangdong Province was remarkable. In order to further promote the integrated development of primary, secondary and tertiary industries in rural areas of Guangdong Province, suggestions were put forward from five dimensions: Comprehensively promoting the prosperity of rural economy, increasing the horizontal and vertical extension of the agricultural industry chain, giving full play to the multi-function of agriculture, promoting the innovative development of agricultural producer services, and building a platform for the integration of innovative industries.
    Rural Revitalization
    Digital rural construction empowers rural industry revitalization: Paths, dilemmas and strategies
    FU Hui-ya
    2023, 62(8):  246-250.  doi:10.14088/j.cnki.issn0439-8114.2023.08.038
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    Industrial revitalization is the core and key to comprehensively promoting rural revitalization. Digital rural construction in the new era, as the strategic direction of rural revitalization and an important measure of digital China, provides a new development opportunity for rural industrial revitalization. Based on this, an analytical framework for enabling rural industry in digital rural construction was constructed by combining practice and experience, namely, basic enabling, subject enabling, technology enabling and resource enabling. It was concluded that while the construction of the digital countryside effectively promoted the transformation and upgrading of rural industries, it also faced the dilemma of imperfect infrastructure, insufficient digital talents, inadequate technology application and lack of resources. It was necessary to put forward optimization suggestions from the perspectives of capital, policy, technology application direction and industrial development orientation.
    The spatial spillover effect of new urbanization on common prosperity
    ZHANG Jie, BAO Xing-yan
    2023, 62(8):  251-257.  doi:10.14088/j.cnki.issn0439-8114.2023.08.039
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    Based on the panel data of 30 provinces (cities, autonomous regions) in China from 2005 to 2020, the entropy-weight TOPSIS model was used to calculate the common prosperity index and the new urbanization index, and the spatial Dubin model was used to reveal the spatial spillover effect of the new urbanization on common prosperity. The results showed that the common prosperity had obvious spatial aggregation characteristics; the improvement of the new urbanization level could not only promote the process of common prosperity in the region, but also have a positive spillover effect on the common prosperity of neighboring regions. In order to achieve the goal of common prosperity, it was necessary to further promote the high-quality development of new urbanization, improve the market transaction system, deepen regional cooperation, promote factor transfer, and strengthen the positive spatial spillover effect.