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Table of Content

    25 May 2023, Volume 62 Issue 5
    Breeding & Cultivation
    Relationship between millet yield and rainfall as well as its main agronomic traits
    NIU Yin-ting, CHEN Pei-yu, JU Le, QIANG Xue-jie, YU Xing-jian, YIN Zhi-gang
    2023, 62(5):  1-4.  doi:10.14088/j.cnki.issn0439-8114.2023.05.001
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    Based on the field performance of millet (Setaria italica Beauv.) variety Yugu 18 in Nanyang City, Henan Province in the past five years, the relationship between rainfall and millet yield was studied. The influencing factors of millet yield were analyzed by correlation analysis and principal component analysis in order to provide a scientific basis for high-yield cultivation techniques of millet in Nanyang City. The results showed that the factors affecting millet yield from large to small were rainfall, ear grain mass, single ear mass, ear diameter, ear length, 1 000-grain weight, growth period, sunshine hours, grain emergence rate, plant height and number of ears. Among them, the yield was negatively correlated with rainfall, 1 000-grain mass, growth period and plant height, and the correlation with rainfall reached a significant level (P<0.05); the yield was positively correlated with other indicators, and the correlation with ear grain mass and single ear mass reached a significant level (P<0.05). Rainfall was negatively correlated with the main agronomic traits of millet such as single ear mass, ear grain mass, ear length and ear diameter. When rainfall was high, the agronomic traits such as ear mass, ear grain mass, ear length, and ear diameter became smaller, and disease such as millet blast, and bacterial brown streak disease was severe, ultimately leading to lower yields. Therefore, a plot or hilly slope with high terrain, flat terrain and waterlogging resistance should be chosen when planting millet in Nanyang City. It is necessary to prevent millet blast and bacterial brown streak disease in years with high rainfall.
    Effects of different densities of Pelophylax nigromaculatus on rice yields and soil fertility in paddy fields
    LI Xing-hua, CHEN Zhan-peng, CAI Zheng-jun, ZOU Cai-qiong, ZHANG Zhong-nan, ZHANG Wen-chao, DING Feng-ju
    2023, 62(5):  5-7.  doi:10.14088/j.cnki.issn0439-8114.2023.05.002
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    In order to optimize the fertilization technology of rice in rice and frog co-cropping mode, a field experiment was conducted with 4 densities of Pelophylax nigromaculatus, and the effects of different densities of Pelophylax nigromaculatus on rice yields and soil fertility in paddy fields were analyzed. The results showed that the effective panicles per unit area increased significantly with the increase of the density of Pelophylax nigromaculatus in the paddy field. Rice yield, soil pH, organic matter and nutrient contents also showed an upward trend as the increase of the density of Pelophylax nigromaculatus. Compared to before the test, soil pH, organic matter, total nitrogen, total phosphorus, alkali-hydrolyzable nitrogen and available phosphorus at the end of one season increased by 2.3%~89.0%, while total potassium and available potassium decreased by 6.9%~24.4%. Therefore, it does not apply nitrogen and phosphorus fertilizer to rice production, but only apply appropriate amount of potassium fertilizer in rice and frog co-cropping mode.
    Resource & Environment
    Spatio-temporal evolution and spatial correlation of carbon emissions in Guiyang City based on land use change
    ZHOU Wei-ting
    2023, 62(5):  8-13.  doi:10.14088/j.cnki.issn0439-8114.2023.05.003
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    Taking Guiyang City as an example, based on the 30 m land cover remote sensing data from 2000 to 2020, the land use transfer matrix, carbon emission calculation model and spatial auto-correlation were used to analyze the spatio-temporal evolution and spatial correlation of land use types and carbon emissions in Guiyang City, to provide reference for the realization of regional “dual carbon” goal and the development of the green and low-carbon economic model. The results showed that from 2000 to 2020, the total carbon emission of land use in Guiyang City had a rapid growth rate on the whole, and the carbon source showed an increasing trend. The carbon source mainly consisted of construction land, and the carbon sink mainly consisted of forest land with only slight fluctuation. In terms of the spatial differences of carbon emissions from 2000 to 2020, the heavy carbon emission areas were mainly concentrated in Yunyan District and Nanming District, and showed a westward expansion trend; the moderate carbon emission areas were mainly concentrated in the southwest and northeast wings of Guiyang City, and showed a northeast shift trend. In terms of spatial correlation, the carbon emission of land use in Guiyang City showed a positive spatial correlation in 2000, and a negative spatial correlation with increasing correlation from 2005 to 2020. The pattern of land use carbon emission in Guiyang City was not optimistic, and the structure and intensity of land use were in urgent need of adjustment. Therefore, feasible low-carbon emission reduction policies should be formulated according to the actual situation of Guiyang City to promote the low-carbon, efficient and sustainable utilization of regional land resources.
    Analysis of influencing factors of farmland fragmentation based on geographical detector: A case study of Guangzhou City
    CHEN Jun-tao, LIN Jin-yao
    2023, 62(5):  14-21.  doi:10.14088/j.cnki.issn0439-8114.2023.05.004
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    Guangzhou was selected as the research area. The landscape pattern index method, comprehensive index method and principal component analysis method were used to construct the evaluation system of farmland fragmentation degree, and the comprehensive index of farmland fragmentation degree of each town and street unit in the region was calculated. Ten quantifiable factors of natural conditions and human social activity were selected, and the geographical detector model was used to detect the impact of 10 factors on farmland fragmentation. The results showed that all 10 factors were the influencing factors of farmland fragmentation in Guangzhou City. The influence of the single factor on farmland fragmentation from strong to weak was urban administrative center distance factor, temperature factor, GDP factor, slope factor and altitude factor, precipitation factor, construction land distance factor, road distance factor, water body distance factor and population density factor. The interaction of any two of the 10 factors showed two-factor enhancement or nonlinear enhancement. Based on the research conclusions, some suggestions were put forward to provide valuable decision-making reference for reducing the level of farmland fragmentation in Guangzhou City.
    Uncertainty and characteristics analysis of forest dynamic changes in the upper reaches of the Yangtze River region based on multi-source land cover data products
    ZHU Chao-fan, ZHU Qiu-an, WANG Le, LIU Jia, QU Li-sha
    2023, 62(5):  22-26.  doi:10.14088/j.cnki.issn0439-8114.2023.05.005
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    Based on the uncertainty of the forest distribution of multi-source land cover data products in the upper Yangtze River region, this paper evaluated the accuracy of multi-source land cover data products using the sixth national forest resource inventory data as a reference, and analyzed the dynamic changes of forests in the upper Yangtze River region. The results indicated that there was significant uncertainty in the distribution and changes of forests in the upper Yangtze River region in land cover data products; the CLUD-A data product had the highest accuracy; the trend analysis of forest change showed that the areas with a significant increase in forest area in the upper Yangtze River region were Chongqing City, Guizhou Province, Sichuan Province, and Yunnan Province; the forest area in the upper Yangtze River region showed an increasing trend from 1982 to 1990, a decreasing trend from 1991 to 2000, an overall increasing trend from 2001 to 2010, and a decreasing trend from 2011 to 2015, which was significantly related to the implementation of the natural conservation project.
    Spatio-temporal evolution and difference of rural settlements in mountainous city:A case study of Chongqing City
    JIANG Wen-jing, LIU Yan, ZHANG Wei, LIU Ming, HE Hui-yu
    2023, 62(5):  27-34.  doi:10.14088/j.cnki.issn0439-8114.2023.05.006
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    Based on the land use change investigation and statistical yearbook of Chongqing City from 2009 to 2018, spatio-temporal evolution of rural settlements was analyzed by using GIS spatial analysis method and landscape pattern index. The results showed that, in scale, the rural settlements in the study area showed a contraction tendency, the size difference between patches decreased, and the micro settlements were the main landscape features. There was an obvious negative correlation between patch size and patch number, and showing the characteristics of a decrease in large patches and an increase in sporadic patches. There was a spatial correlation in the scale of rural settlements, with two types of agglomeration: high-high agglomeration and high-low agglomeration. In spatial distribution, the rural residential areas in the study area mainly exhibited a distribution pattern of dense in the west and sparse in the east, and the distribution characteristics of “large diaspora and small settlement”. Besides, the rural settlements were mainly distributed in low altitude areas, but it was not significantly affected by the first and second-class rivers of the Yangtze River. The overall pattern had not changed significantly in the past decade. In morphology, the morphology of rural residential areas in the study area tended to be irregular, indicating that the planning guidance function was not strong, and there were obvious spatial differentiation characteristics in space.
    Temporal and spatial evolution characteristics and influencing factors of vegetation coverage in Oasis of Makit County from 1990 to 2020
    DENG Jiang
    2023, 62(5):  35-43.  doi:10.14088/j.cnki.issn0439-8114.2023.05.007
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    The evolution characteristics and influencing factors of vegetation coverage in Oasis of Makit County from 1990 to 2020 were analyzed using Landsat series data. The results showed that the vegetation in Oasis of Makit County was generally adjacent to the Yerqiang River and the Tizinapu River. The vegetation coverage from the inside to the outside of the oasis was from the first level to the third level, and the fourth level covered a large area in the west of the oasis. From 1990 to 2020, the total vegetation coverage area, annual average NDVI, and average vegetation coverage in Oasis of Makit County showed an upward trend, and the total vegetation coverage area increased by 199.9 km2, with an increase of 12.26%. The absolute spatial variation of vegetation coverage in Oasis of Makit County had the characteristics of temporal and regional differences, both alone and in common. From 2010 to 2020, the area of the first level improvement area of vegetation coverage increased most significantly, which was a typical temporal change; the reclamation area of the 45th Oasis Corps and the northeast of Korma Township had been improved most obviously, and the large-scale degradation around Makit Town was also obvious, which was a typical regional change. From 1990 to 2020, the vegetation coverage intensity and speed in Oasis of Makit County were 0.4%/a and 6.4 km2/a respectively. During the 31 years, except for the overall migration of the fourth level coverage to the southeast, the total vegetation coverage, the first level coverage, the second level coverage and the third level coverage all migrated to the northeast. The increase of cultivated land area in Oasis of Makit County was the main driving force for the increase of oasis vegetation coverage degree, but the increase of cultivated land water demand had squeezed out the ecological irrigation water resources of natural plants in desert oases, leading to the degradation of natural plants, and threatening the harmonious balance of desert oasis ecology.
    Spatio-temporal research on the rationality of land use structure in Suihua City based on improved TOPSIS method
    ZHAO Shu-juan, ZHAO Qian, JI Tian-ci, YANG Hui-xian
    2023, 62(5):  44-48.  doi:10.14088/j.cnki.issn0439-8114.2023.05.008
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    The rationality of land use structure in Suihua City of Heilongjiang Province from 2010 to 2019 was studied by using the improved TOPSIS method. The results showed that, from 2010 to 2019, the rationality of land use structure in Suihua City was relatively high, and fluctuated. The rationality of land use structure was the lowest in 2010, and the highest in 2017, reaching 100.00%. So, in the future, the land use structure could be adjusted according to 2017. Different land use types had different impacts on the rationality of land use structure, urban and rural construction land had the highest correlation with the rationality of land use structure, the grassland had the lowest correlation with the rationality of land use structure. In 2011, the rationality of land use structure in Suihua City was above 95%, Mingshui County had the lowest rationality, and Qinggang County had the highest rationality, reaching 98.85%. According to the results, some measures were put forward to optimize the land use structure of Suihua City, including controlling the use of urban and rural construction land and transportation and water conservancy land, protecting cultivated land, appropriately developing natural reserves, and adjusting the proportion of forest land, grassland, garden land and facility agricultural land.
    Bacterial diversity and community composition in the coastal intertidal zone sediments of Lianyungang City
    YAO Jin, LIU Yi-wei, ZHANG Ji-luan, LI Jia-le, WU Da-li, WANG Hong-bin
    2023, 62(5):  49-53.  doi:10.14088/j.cnki.issn0439-8114.2023.05.009
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    Based on the 16S rRNA gene, high-throughput sequencing technology was used to analyze bacterial diversity and community structure in coastal intertidal zone sediments of Lianyungang City, Jiangsu Province. Three sampling points were set up in three typical coastal intertidal zones of Lianyungang City, and nine sediment samples were collected. Bacteria were enriched and cultured, and total DNA was extracted. IIIumina Miseq sequencing was conducted and sequencing results were analyzed. The results showed that the bacteria in the three sampling points were mainly composed of Proteus, Bacteroides, Firmicutes, Actinomycetes, Acidobacteria, Fusobacteria, Verruca, Bacillus, Planctomycetes, and Chloroflexi. Among them, Proteus was the dominant bacteria group, accounting for 99.4% of the total bacteria group. The diversity and richness of bacterial communities were as follows: Xugou sampling point>Xishu sampling point>Dongxilian Island sampling point.The principal component analysis of bacterial community structure and the sample cluster analysis of UniFrac distance were consistent with the environmental and ecological differences of the sample collection points. The different types of sediment and physical and chemical factors in the intertidal zone affected the bacterial diversity and community composition.
    Correlation between soil nutrient content and enzyme activity in Betula luminifera forests of different forest ages
    YU Chun-he, ZENG Zhu, WEI Qiu-si, LIU Shi-nan, YANG Mei
    2023, 62(5):  54-58.  doi:10.14088/j.cnki.issn0439-8114.2023.05.010
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    In order to explore the soil nutrient management and nutrient cycling of Betula luminifera forests of different forest ages, the soil of the Betula luminifera forests in the northwest of Guangxi Zhuang Autonomous Region at 3, 7, and 13 years old was used as the research object. The soil nutrient characteristics, soil enzyme activity characteristics, and the correlation between soil nutrient and soil enzyme activity were analyzed in the 0~20 cm and 20~40 cm soil layers of different forest ages. The results showed that in the 0~20 cm soil layer, the nutrient content and enzyme activity of Betula luminifera forests of different forest ages were higher than those in the 20~40 cm soil layer; as the forest age increased, except for the total phosphorus content and sucrase activity in the soil, all other indicators first decreased and then increased, reaching their maximum at 13 years old; the Betula luminifera forest showed a significant decrease in soil nutrient content and enzyme activity during the fast-growing period (7 a). Research had shown that there was a close relationship between soil enzyme activity and soil nutrient content. Among them, the activities of catalase and urease were highly significantly positively correlated with ammonia nitrogen, available potassium, and available phosphorus in the soil, while the correlation between total phosphorus content and these four enzyme activities was relatively small. To sum up, forest age had a significant impact on the changes of soil nutrients and enzyme activities in Betula luminifera forest. With the increase of forest growth on the uptake of soil nutrients, soil fertility would decline.It was necessary to increase the input of nitrogen, phosphorus, and potassium elements through fertilization measures in the early stage of Betula luminifera forest, in order to meet the demand for soil nutrients in the fast-growing stage of Betula luminifera forest and accelerate forest growth.
    Process properties and microbial diversity during the statical aerobic composting of food waste
    ZHANG Xuan, DENG Xiu-shan, DENG Xiu-quan, MA Da-chao, WU Yan-ping, FENG Qing-ge
    2023, 62(5):  59-65.  doi:10.14088/j.cnki.issn0439-8114.2023.05.011
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    A new compost bin was used for statical aerobic composting of food waste. Changes of physicochemical parameters and the succession of the microbial community during the composting process were investigated. It was found that the moisture content, organic matter, and C/N ratio of the compost decreased, and contents of total nutrients remained largely the same. The organic matter, total nutrients, moisture and pH of the final compost product were 66.2%, 7.49%, 18.5% and 7.70 respectively. The properties complied with the requirements of regulation according to Organic Fertilizer (NY/T 525—2021). Firmicutes, Actinobacteria and Proteobacteria were the major bacterial phyla during the composting process, while Ascomycota was the dominant phylum of fungi. The bacterial community was mostly composed of Virgibacillus and Staphylococcus during the inception phase and high temperature stage of composting. However, the predominant bacteria quickly changed to Sphingomonas in the mesophilic cooling stage of composting, and microbial diversity and species richness were high at that time.
    Plant Protection
    Antifungal activity of chemical constituents from Mikania micrantha against 6 species of Juglans regia pathogenic fungi
    ZHANG Wei, QI Jin-kang, LI Jin-fang, HU Shi-jun, YAN Xiao-hui
    2023, 62(5):  66-72.  doi:10.14088/j.cnki.issn0439-8114.2023.05.012
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    In order to explore the antifungal effects of the chemical constituents of invasive Mikania micrantha, five compounds were isolated from the ethyl acetate extract of the aerial part of Mikania micrantha by chromatographic methods such as silica gel column chromatography and Sephadex LH-20 gel column chromatography. According to their 1H NMR, 13C NMR and MS spectral data, the five isolated compounds were identified as Stigmasterol(1), Mikanolide(2), Deoxymikanolide(3), 3,5,4′-trihydroxy-6,7-dimethoxy-flavone(4) and Nepetin(5), respectively. The antifungal activities of different solvent extracts and isolated compounds from Mikania micrantha against 6 species of pathogenic fungi of walnut were determined by the petri plate method. The results showed that the ethyl acetate extract and the n-butanol extract had a high inhibitory effect on the mycelia growth of Colletotrichum sp. and Phyllosticta sp., with EC50 of 1.250 00 mg/mL and 1.660 00 mg/mL, respectively. Compounds 2 and 3 showed higher inhibition rats against Phompsis sp. and Fusicoccum sp. than other compounds. The EC50 of compounds 2 and 3 against Phompsis sp. were 60.62 μg/mL and 89.13 μg/mL, and against Fusicoccum sp. were 43.65 μg/mL and 61.66 μg/mL, respectively.
    A preliminary study on predation behavior of Eocanthecona furcellata on the larvae of Agriophara rhombata
    GONG Xue-na, YU Xiang-shuai, WANG Xue-song, LONG Li-xue, LI Xiao-xia, LONG Ya-qin
    2023, 62(5):  73-76.  doi:10.14088/j.cnki.issn0439-8114.2023.05.013
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    The predation behavior of Eocanthecona furcellata on Agriophara rhombata larvae was studied, and the search and predation efficiency of different instars of Eocanthecona furcellata nymph on Agriophara rhombata larva was measured in the laboratory. The results showed that Eocanthecona furcellata nymphs searched for prey by swinging their antennae and used the retractable oral needle in their beak as the hunting and feeding tool. The predation effect of Eocanthecona furcellata nymphs was selective to Agriophara rhombata larvae, and the older nymphs had higher predation efficiency. Among them, the 5th instar of Eocanthecona furcellata nymphs had the highest predation efficiency on the 3rd instar of Agriophara rhombata larvae, which could search for the prey in about 5 minutes on average, successfully imbibe and start feeding in about 13 minutes, and it could suck dry one larva in 45 to 60 minutes; the density of the two species interfered with their predation ability.
    Horticulture & Local Products
    Effects of meteorological factors on the quality of Qianyang Bingtang orange
    JIANG Di-fei, LI Yue-yong, DONG Zi-zhou, LI Hao, TAN Shi-qi
    2023, 62(5):  77-82.  doi:10.14088/j.cnki.issn0439-8114.2023.05.014
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    Based on the historical meteorological observation data of Hongjiang City from 2015 to 2019 and the quality detection data of Qianyang Bingtang orange, the correlation between seven quality indexes of Qianyang Bingtang orange and meteorological factors were analyzed by Pearson correlation analysis and stepwise regression analysis. The results showed that, the key meteorological factors affecting the titratable acid content of Qianyang Bingtang orange were the daily maximum temperature in strong fruit period and the daily minimum temperature in coloring period; the ratio of solid to acid was related to sunshine hours in fruit expansion period, cloudy and rainy days in strong fruit period, average maximum temperature in picking period and precipitation in picking period; the content of soluble solids was positively correlated with the daily temperature range during fruit expansion and sunshine hours during picking, and was negatively correlated with the daily average minimum temperature in fruit expansion period and cloudy and rainy days in coloring period; the key influencing factors of VC content were daily minimum temperature and daily maximum temperature in fruit expansion period, cloudy and rainy days in coloring period and sunshine hours in picking period; the key factors affecting the edible rate were the daily range during picking and the sunshine during fruit expansion; the key meteorological factors affecting the juice yield were the average daily minimum temperature, precipitation in the picking period and the average daily maximum temperature in the strong fruit period; the transverse diameter was related to the daily average minimum temperature and daily temperature range during fruit expansion, cloudy and rainy days during coloring and sunshine during picking. Based on the correlation analysis, the climate quality evaluation model of various quality indexes was constructed, and the model was verified by using the historical meteorological data and quality detection data in 2020. The relative error range was 1.2%~16.7%. The verification results showed that the model could exactly simulate the climate quality of Qianyang Bingtang orange.
    Analysis on growth and soil physical and chemical properties of Betula luminifera plantation under different site conditions
    LI Song-hai, LU Zhi-feng, LIU Shi-nan, YANG Mei, WEI Qiu-si
    2023, 62(5):  83-87.  doi:10.14088/j.cnki.issn0439-8114.2023.05.015
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    The experiment was conducted to study the growth and soil physical and chemical properties of Betula luminifera plantation under different site conditions, providing reference for cultivation and promotion of Betula luminifera plantation and cultivation of large-diameter timber in southern mountainous areas. Four standard plots of different site conditions including sunlit slope (top and bottom) and shaded slope (top and bottom) were set up in Betula luminifera plantation, and tree height, DBH, height under branches and crown width were measured under different site types. The soil enzyme activity and nutrient content were measured. Single factor analysis of variance(ANOVA) was used to analyze the differences of growth and soil physical and chemical properties of Betula luminifera plantation in different types. Pearson correlation coefficient and principal component analysis (PCA) were used to comprehensively evaluate the soil quality of Betula luminifera plantation under different site types. The results showed that, the growth of tree height, DBH, height under branches and crown width of Betula luminifera plantation at the lower and upper parts of the sunny slope was better than that at the lower and upper parts of the shady slope. Under different site types, there were significant differences in soil enzyme activity and soil nutrient content of Betula luminifera plantation(P<0.05). Generally, the lower part of the sunny slope was higher than the upper part of the sunny slope, and the lower part of the shady slope was higher than the upper part of the shady slope. With the decline of slope position, the soil fertility of Betula luminifera plantation increased. The results of correlation analysis showed that the growth of tree height, DBH and crown width of Betula luminifera plantation was highly positively correlated with most soil enzyme activities and soil nutrient indexes (r>0.800), indicating that the growth of Betula luminifera plantation was consistent with the spatial change of soil fertility. Principal component analysis showed that the comprehensive score of soil quality of Betula luminifera plantation under different site types from high to low was lower part of sunny slope > upper part of sunny slope > lower part of shady slope > upper part of shady slope. Different slope positions and directions had significant effects on the growth and soil fertility of Betula luminifera plantation. The lower part of the sunny slope was the best area for the growth of Betula luminifera plantation.
    Investigation of soil physical and chemical indicators and content of alkaloid of Hechi wild Sophora tonkinensis and their correlation analysis
    SU Jiang, CEN Zhong-yong, XIE Yan-jun
    2023, 62(5):  88-91.  doi:10.14088/j.cnki.issn0439-8114.2023.05.016
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    In order to study the relationship between the nutrient requirement and the alkaloid content of wild Sophora tonkinensis Gagnep., Fengshan County, Luocheng County and Yizhou City of Hechi City were taken as the sample point. The pH, nitrogen, phosphorus, potassium, organic matter of soil and alkaloid content of wild Sophora tonkinensis were investigated, and their correlation was analyzed. The results showed that the organic matter content and nitrogen content of soil of Fengshan County were the highest. The total phosphorus and available phosphorus content of soil of Yizhou City was the highest, and that of Fengshan County was the lowest. The total potassium content of soil of Luocheng County was the highest, and that of Fengshan County was the lowest. The content of available potassium of soil of Yizhou City was the highest, and that of Fengshan County was the lowest. The alkaloid content of Sophora tonkinensis of Yizhou City was the highest, with 9.96 mg/g, which was higher than that of Fengshan County and Luocheng County. The result of correlation analysis showed that there was a positive correlation between alkaloid content of wild Sophora tonkinensis and the pH, organic matter content, total phosphorus, available phosphorus, total potassium, and available potassium content of soil of different habitats, while the correlations between alkaloid content of wild Sophora tonkinensis and total nitrogen, available nitrogen content of soil were negative. Therefore, reasonable fertilization should be made in production accordance to the actual conditions.
    Knowledge mapping analysis of Chinese flower border research based on CiteSpace
    HUANG Jia-cheng, LIU Hong-tao, CHEN Yuan-yuan, LYU Wen-jun, LUO Jing
    2023, 62(5):  92-99.  doi:10.14088/j.cnki.issn0439-8114.2023.05.017
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    The literature on flower border published from 1981 to 2021 were collected from the CNKI database as the research object. The knowledge map was drawn by CiteSpace scientific knowledge visualization software, and the authors, research institutions, keywords were analyzed in combination with literature analysis, so as to sort out the development and tendency of Chinese flower border research. The results showed that the research stage of Chinese flower border research could be divided into accumulation period (1981—2000), growth period (2001—2018) and decline period (2019—2021); the research group was in the situation of “overall dispersion and partial concentration”, and the extensive cooperative relationship between research institutions had not been established; Chinese flower border research could be divided into four aspects: Flower border design concept research, flower border plants research, flower border application research and flower border construction management research, and the relatively stable research system had been formed; the current research on flower border in China mainly focused on the application and plants research under different cities, environments and scenes, and functional flower border with different characteristics such as low maintenance or rainwater regulation. Based on historical background such as urban biodiversity conservation, carbon neutrality and peak carbon dioxide emissions, exchanges and cooperation should be strengthened, and the follow-up study should be carried out from the perspective of multidisciplinary integration.
    Effects of tourism disturbances on plant diversity of geomantic forests in Wuyuan
    WANG Lei, HUANG Zhi-qiang, ZHAI Jian-cheng, GUO Fu-sheng
    2023, 62(5):  100-106.  doi:10.14088/j.cnki.issn0439-8114.2023.05.018
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    Wuyuan, a popular village for rural tourism and an ancient village with well-preserved geomantic forests, was selected for the study of the effects of tourism activities on the biodiversity of village geomantic forests through field sample surveys, and comparative analysis of disturbance factors and biodiversity. The results showed that the average height of shrubs and herbs decreased significantly, and the health of vegetation and the number of tree and herb species decreased; in terms of biodiversity, the total Simpson, Shannon-Wiener and Pielou evenness indices decreased and the total Patrick index increased; in terms of community stability, the negative impact of tourism on community stability was dominant, and the impact was not significant. With the continued increase in tourism popularity in Wuyuan, it was important to do a good job of developing and protecting the geomantic forest from planning and management, monitoring and filing, mobilizing local residents and popularizing science to tourists.
    Effects of chitosan on nutrient absorption of grape seedlings
    HUANG Yong, CHEN Song, CHEN Qing-dong, CHEN Qing-hua, FAN Zhong-han, XIA Dan, LIAO Ming-an, LIN Li-jin, HU Rong-ping
    2023, 62(5):  107-111.  doi:10.14088/j.cnki.issn0439-8114.2023.05.019
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    In order to screen out the most optimal chitosan concentration for grape(Vitis vinifera L.) seedling nutrient absorption and provide the reference for grape production, grape seedlings were used as materials in the experiment, the chitosan with different concentrations was sprayed on the grape seedlings, and the effects of chitosan on nutrient absorption of grape seedlings were studied. The results showed that the chitosan increased the contents of nitrogen(N), phosphorus(P), and potassium(K)in the whole plant of grape seedlings. When the concentration of chitosan was 4 g/L, the content of N, P and K reached the maximum, increasing by 25.53%, 18.67% and 12.63% compared with that of the control, respectively. The chitosan treatment also increased the concentrations of soil alkali-hydrolyzable N, available P, available K and organic matter, and the content of soil organic matter was increased by 42.58% when the concentration of chitosan was 4 g/L. With the increase of chitosan concentration, the medium trace elements sodium and calcium contents in grape seedlings had a trend of decrease, increase, and then decrease, and the magnesium content in grape seedlings had a trend of increase and decrease. For soil enzyme activity, chitosan increased the activities of soil sucrase, urease, catalase, and phosphatase. Chitosan at 4 g/L was the most beneficial to grape seedlings nutrient absorption.
    Spatial evaluation of village streets and lanes under the Great Heritage Relic Protection——A case study of Jiangwudian Village in Xi'an City
    ZHANG Dan, FENG Yan, ZHANG Ling, WANG Dong-meng, KONG De-zheng
    2023, 62(5):  112-117.  doi:10.14088/j.cnki.issn0439-8114.2023.05.020
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    Taking Jiangwudian Village in Xi'an City as the research object and taking into account the conservation policy of the Great Heritage Relic Protection and related planning, the corrected axes were imported into Depthmap X software based on the theory of spatial syntax, and quantitative analysis was carried out on the connection value, integration, selection and comprehensibility. The results showed that the spatial permeability of Jiangwudian Village was weak due to the concentrated layout of houses and the villagers' focus on overall living and transportation; the villagers' daily travel was restricted due to the development of public transport, which resulted in a poor overall integration degree and a high local integration degree; the overall selection degree of the village was low, which had a great influence on the external reception potential of the village; the comprehensibility of villages was low, and it was difficult for outsiders to perceive and understand the structure of villages. The public service facilities of the village needed to be improved, the function of the spatial nodes of the village should be strengthened, a comfortable and pleasant local green space should be created in the context of the Great Heritage Relic Protection, and the accessibility and traffic guarantee function of the village should be improved, so as to provide a suitable spatial environment for the future development of the village.
    Animal Husbandry & Veterinary Medicine
    The effects of ampelopsin on lipopolysaccharide-induced acute lung injury mice and the TLR4/MyD88/NF-κB signaling pathway
    ZHU Hai-bin, FANG Jing, TANG Mu-lan, CHI Xin-yu, ZENG Chun-hui, YANG Ke
    2023, 62(5):  118-123.  doi:10.14088/j.cnki.issn0439-8114.2023.05.021
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    Aiming to investigate the effects of ampelopsin (APS) on lipopolysaccharide (LPS)-induced acute lung injury in mice and its underlying mechanism, the acute lung injury mouse model was established by intratracheal instillation of LPS. Seventy-two KM mice were randomly divided into 6 groups as follows: normal, model, dexamethasone and APS high-dosage, medium-dosage, and low dosage group. After 8 h of LPS induction, the animal lung function analysis system was employed to monitor pulmonary function (airway resistance, pulmonary dynamic compliance and minute ventilation volume) of mice. ELISA assay was carried out to measure the content of inflammatory factors TNF-α and IL-1β. Colorimetric assay was performed to determine MPO activity. H&E staining was performed to reveal pathological changes in lung tissues. Western blotting was performed to measure the protein expression of TLR4, MyD88, IκBα, p-IκBα, p65 and p-p65. Results showed that APS administration decreased airway resistance and increased pulmonary dynamic compliance and minute ventilation volume compared to those in the model group. Also, APS reduced TNF-α, IL-1β content and MPO activity. Further, Western blotting results demonstrated that APS down-regulated the protein expression of TLR4, MyD88, p-IκBα, p65 and p-p65, and up-regulated IκBα protein expression in LPS-induced acute lung injury. APS exerted a protective effect on LPS-induced acute lung injury in mice through regulating the TLR4/MyD88/NF-κB signaling pathway.
    Protective effect of baicalin on porcine extra-intestinal pathogenic Escherichia coli in vivo
    REN Ming-xing, XIAO Yong, FU Shu-lin, ZONG Bing-bing, QIU Yin-sheng
    2023, 62(5):  124-128.  doi:10.14088/j.cnki.issn0439-8114.2023.05.022
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    To explore the protective effect of baicalin on mice infected with porcine extraintestinal pathogenic Escherichia coli, the survival rate assay and colonization assay of mice were selected to evaluate the protective effect of baicalin. The clinical feature, survival rate, tissue bacterial load and pathological changes of mice in the infected group and baicalin treatment group were compared. The results showed that the mental state of mice treated by baicalin was better than that of the mice infected by PCN033. The survival rate of the baicalin treatment group was improved compared with that of the infection group. Bacterial counts in heart, liver, spleen and kidney of mice treated with baicalin were significantly reduced compared with those of mice infected with PCN033 (P<0.01).
    Prokaryotic expression of recombinant early pregnancy factor and preparation of polyclonal antibody
    WANG Cong, WANG Han, ZHANG Hai-ling, XIAO Yi-ran, GUO Yu-xi, LIU Meng-di, LI Hao-song, WU Zong-cheng, DUAN Fu-chun, LU Shi-ying
    2023, 62(5):  129-134.  doi:10.14088/j.cnki.issn0439-8114.2023.05.023
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    Bioinformatics analysis of bovine early pregnancy factor was conducted, and prokaryotic expression vector pET-28a-srEPF containing pro-soluble labels was constructed. The prokaryotic expression vector was successfully induced by IPTG, and the early pregnancy factor was purified by affinity chromatography. Then, recombinant protein was used as immunogen to immunized animals to prepare polyclonal antibodies. The expressed proteins and their polyantibodies were detected by Western blotting. The results showed that the recombinant protein of early pregnancy factor had been successfully prepared, and its molecular weight was about 26 ku. After purification, the concentration was determined by BCA method to be 1.1 mg/mL. Both the recombinant protein of early pregnancy factor and EPF protein after enzyme digestion could be specifically bound to the prepared polyclonal antibody, and the titer determined by indirect ELISA was 1:256 000.
    Storage & Processing
    Research on the extraction of flavonoids from Chenopodium quinoa and its antioxidant activity
    LU Xiao-fang, LI Ya-nuo, SONG Bing-ze, YANG Mei-hong, YUE Ai-qin, ZHAO Jin-zhong
    2023, 62(5):  135-141.  doi:10.14088/j.cnki.issn0439-8114.2023.05.024
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    Taking the seeds of Chenopodium quinoa as the research object, based on ultrasonic time, solid-liquid ratio, and negative pressure of single factor experiment, combined with the characterization results of SEM of Chenopodium quinoa powder, the optimal extraction process condition of flavonoids from Chenopodium quinoa was obtained by response surface optimization. The crude extract of flavonoids from Chenopodium quinoa was extracted with ethyl acetate and purified by the silica gel column (The eluent was acetone) to obtain the flavonoids with high purity, which was tested for antioxidant activity in vitro. The results showed that the best process parameter for the extraction of flavonoids from Chenopodium quinoa by ultrasonic-negative pressure was ultrasound time of 27 min, solid-liquid ratio of 1:36(g/mL) and negative pressure of 0.059 MPa. The extraction rate (5.31 ± 0.038) mg/g was close to the predicted extraction value of 5.28 mg/g. The factors affecting the extraction rate of flavonoids from Chenopodium quinoa were solid-liquid ratio> negative pressure> ultrasonic time. The antioxidant activity of flavonoids from Chenopodium quinoa was positively correlated with the concentration, and the greater the concentration, the stronger the antioxidant capacity. At a concentration of 0.25 mg/mL, the scavenging rate of the DPPH radical reached (89.5 ± 0.430)%.
    Effect of industrial fermentation on neutral aroma components of cigar core
    PAN Yong, LI Lin-lin, FANG Xin, ZHU Ting-ting, ZHOU Ya-bin, HU Jie, WANG Rong-hao, LIU Yang, TAN Zai-yu, YANG Mei, HUANG You-yi, SHI You-zhi
    2023, 62(5):  142-147.  doi:10.14088/j.cnki.issn0439-8114.2023.05.025
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    In order to explore the effect of industrial fermentation on neutral aroma components of cigar core, the neutral aroma components of cigar core samples in five stages from raw material to pile out were detected by simultaneous distillation extraction-gas chromatography-mass spectrometry. The results showed that 59 neutral aroma components were detected in the industrial fermentation of cigar core, mainly ketones and alcohols. During the fermentation process, the number of types of neutral aroma components in cigar core did not change significantly; the total amount of neutral aroma components decreased first and then increased, and the content increased significantly on the whole; the changes of neutral aroma components were mainly occurred between the raw materials and the early fermentation stage, as well as between the late fermentation stage and the pile production stage, while there was little difference in neutral aroma components of the samples in the early, middle and late fermentation stage. A total of 14 different neutral aroma components were screened out before and after industrial fermentation, including solanone, phytone, farnesyl acetone, 8S,14-cypress glycol, cypress brain, (1R,2E,4S,7E,11E)-4-isopropyl-1,7,11-trimethyl-2,7,11-cyclotetraene-1-alcohol, vegetable alcohol, neophytadiene, etc. The above results showed that the industrial fermentation could significantly affect the content of aroma substances in cigar core, which provided a theoretical basis for understanding the formation of aroma quality of cigar core in industrial fermentation.
    Detection Analysis
    Differences in nutritional composition of peony seed oil in different regions of Guizhou Province
    LEI Lu, ZHOU Jing-rui, ZHANG Qin, MA Chao
    2023, 62(5):  148-152.  doi:10.14088/j.cnki.issn0439-8114.2023.05.026
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    In order to explore the differences in the quality of peony seed oil in different regions of Guizhou Province, 10 representative regions were selected and peony seed oil was extracted by pressing method. The oil content, physicochemical indicators, polyphenols, trace elements, and fatty acids of peony seeds were analyzed. The results showed that the origin had little effect on the physicochemical indicators (relative density, acid value, saponification value, etc.) of peony seed oil, but had a significant impact on the oil content, Fe, Cu, Zn, Ca elements, and fatty acid content. Among them, sample MDY4 had the highest oil content at 33.75%, with Fe, Cu, and Zn elements at 9.42, 4.29, and 55.04 mg/kg, respectively. The content of oleic acid and α-linolenic acid was 116.25 and 150.39 mg/g, respectively, which was the highest among all samples. Sample MDY1 was second only to MDY4. Therefore, Hezhang County in Bijie City and Sinan County in Tongren City could be used as promotion planting areas and breeding areas for oil peonies.
    Analysis of endogenous harmful components in ginkgo seed kernel and fried ginkgo seed kernel
    ZHANG Yan-miao, DING Ming-he, ZHANG Ying, TAN Zhong-chuan, ZHOU Tian-tian, GAN Guo-ping
    2023, 62(5):  153-159.  doi:10.14088/j.cnki.issn0439-8114.2023.05.027
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    Ginkgo seed kernel and fried ginkgo seed kernel were used as experimental materials for the determination of total ginkgolic acid using HPLC. The chromatographic column was YMC-Triart C18 (4.6 mm×150 mm,5 μm). The mobile phase was acetonitrile solution with 0.1% trifluoroacetic acid -0.1% trifluoroacetic acid aqueous solution, with a detection wavelength of 310 nm and a flow rate of 1.0 mL/min; 4′-O-methyl pyridoxine (MPN) and 4′-O-methoxy pyridoxin-5′- glucoside (MPNG) were determined by HPLC on YMC Triart C18 (4.6 mm×150 mm,5 μm). The mobile phase was methanol deionized water, with a detection wavelength of 328 nm and a flow rate of 1.0 mL/min. Headspace gas chromatography (HS-GC) was used to perform a limit check on hydrocyanic acid. The results showed that the average recovery rate of total ginkgolic acid was 101.17%, RSD was 4.57% (n=6), the quantification limit was 12.18 ng, and the detection limit was 4.87 ng; the average recovery rate of MPN was 103.14%, RSD was 4.80% (n=6), the quantification limit was 5.94 ng, and the detection limit was 2.38 ng; the average recovery rate of MPNG was 103.54%, RSD was 3.05% (n=6), the quantification limit was 11.20 ng, and the detection limit was 4.48 ng; the quantitative limit of hydrocyanic acid was 10 ng, and the detection limit was 3.5 ng. The established method had strong specificity, good repeatability, and high sensitivity, and could be used for the detection of endogenous harmful components in ginkgo seed kernel and fried ginkgo seed kernel.
    Differences in volatile components of valerian essential oil from different years
    ZHOU Jing-rui, JIANG Ling-ling, LEI Lu, RAN Jiang, AI Rong, LUO Wen-ju, ZHONG Xue-zhi, WANG Xin, YU Bo
    2023, 62(5):  160-164.  doi:10.14088/j.cnki.issn0439-8114.2023.05.028
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    The volatile components of valerian(Valeriana officinalis L.) essential oil from 2020 and 2021 were analyzed using gas chromatography-time of flight mass spectrometry. The relative content of each component was calculated using the peak area normalization method, and the compositional characteristics of volatile components of valerian essential oil from two years were studied.The results showed that a total of 70 main volatile components were identified in the two years of valerian essential oil, with the highest relative content of diene compounds. There was a significant difference in the volatile components of valerian essential oil between two years, with more types of volatile components present in 2021 compared to 2020. Among the common volatile components, the relative content of 2-pinene, camphene, β-Pinene, 1-limonene, endobornyl acetate, myrtenyl acetate, methoxyacetic acid, and β-terpinyl acetate was high.
    Biological Engineering
    Comparative analysis of the transcriptome of kiwifruit ‘Qihong' and ‘Xuxiang' during the overwintering period
    MAO Ke-xin, WANG Hai-rong, AN Miao, LV Wei, LI Jian, LI Guo-tian
    2023, 62(5):  165-171.  doi:10.14088/j.cnki.issn0439-8114.2023.05.029
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    Chinese kiwifruit (Actinidia chinensis) ‘Qihong' and delicious kiwifruit (Actinidia deliciosa) ‘Xuxiang' with different cold tolerance were used as materials.Samples were taken during the overwintering period and analyzed for physiological parameters, and it was found that ‘Qihong' was more tolerant to low temperatures than ‘Xuxiang'. The transcriptomes of the two varieties were analyzed and the GO clusters showed greater differences in cellular components organization or biogenesis, organelle fraction, etc;KEGG clusters differed more in membrane transport, translation, transcription, replication and repair. The enrichment analysis of the 2 varieties showed differential expression of starch and sucrose metabolism, phytohormone signaling, biosynthesis of phenylpropanoids and biosynthesis of flavonoids pathways. The changes in transcript levels of the two varieties during the overwintering period were analyzed.
    Identification of genes related to lipopeptide synthesis in mining lipopeptide synthesis related genes of Bacillus amyloliquefaciens TF28 under different carbon sources based on transcriptome
    YAN Geng-xuan, WANG Xiang-xiang, TIAN Yuan, LIU Zhi-ting, ZHANG Shu-mei, XIA Hai-hua
    2023, 62(5):  172-178.  doi:10.14088/j.cnki.issn0439-8114.2023.05.030
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    With Bacillus amyloliquefaciens TF28 as the test strain, three experimental groups were set up: glucose group (control), fructose group and xylose group. Differential genes were identified by transcriptome sequencing, and functional analysis was carried out on the differential genes to explore the regulatory genes for lipopeptide synthesis. A total of 688 differentially expressed genes, 522 upregulated genes, and 166 downregulated genes were identified in the fructose group; a total of 855 differential genes, 691 upregulated genes, and 164 downregulated genes were identified in the xylose group. Different carbon sources changed the expression levels of global regulatory factors in the quorum sensing system and dual component system of lipopeptide synthesis in starch Bacillus subtilis TF8, and influenced the synthesis and metabolism of essential amino acids and fatty acids in lipopeptide synthesis, which provided reference for further research on the biological regulatory mechanisms of lipopeptide synthesis.
    Analysis of mitochondrial COI sequences from different populations of Macrobrachium nipponense japonicus
    HUANG Hui, LIU Kai, WANG Yu-xi, GUO Wei, MA Heng-jia, XIE Nan
    2023, 62(5):  179-182.  doi:10.14088/j.cnki.issn0439-8114.2023.05.031
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    Macrobrachium nipponense samples from five different populations including Qiantang River (QT), Wuyi (WY), Gaotanghu (GT), Zhaoqing (ZQ) and Nanning (NN) were collected in the experiment, and the mitochondrial COI gene of each population was sequenced and analyzed to understand the genetic differences among populations. The results showed that in the COI gene sequence of Macrobrachium nipponense, there were 420 conserved sites, 222 mutated sites, 123 reduced information sites, and 27 base deletion or insertion sites, with an average conversion to inversion ratio of 2.78. The content of (A+T) in the COI gene sequence was significantly higher than that of (G+C), and the base composition exhibited significant AT bias. 65 haplotypes were defined in 67 individuals, and haplotypes from NN and QT populations formed two main branches. The phylogenetic tree and cluster tree constructed based on genetic distance showed that the genetic distance between QT population and GT population was relatively close, the genetic distance was relatively far compared with ZQ population and NN population, and the genetic distance between WY population and QT population and GT population was relatively small. The research results could provide a molecular biological basis for the rational development and utilization of Macrobracium germplasm resources.
    Economy & Management
    Research on the market structure of China's rural e-commerce platform based on SCP analysis paradigm
    LIANG Yue-ze, QIAO Peng-hui, WANG Hui
    2023, 62(5):  183-189.  doi:10.14088/j.cnki.issn0439-8114.2023.05.032
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    With SCP analysis paradigm as the basic theoretical framework, data analysis and empirical research were used to analyze the current situation of market structure, market behavior and market performance of rural e-commerce platforms, and to study the impact of market structure on market behavior and market performance. The results showed that there were problems such as insufficient platform differentiation, imperfect agricultural financing system, high cost and difficulty in making profits under the high oligopoly market structure of rural e-commerce platform, and the suggestions of market structure optimization, such as creeting the brand of rural e-commerce platform, strengthening division of labor and cooperation between platforms and rebuilding the new ecological system were put forward.
    Energy efficiency evaluation of tertiary industry in Yangtze River Economic Belt
    CHEN Hong, ZHANG Hua-wei
    2023, 62(5):  190-195.  doi:10.14088/j.cnki.issn0439-8114.2023.05.033
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    Using the Min-DS model and Kernel density estimation function, the energy efficiency of tertiary industry in 11 provinces and cities along the Yangtze River Economic Belt was measured and its spatio-temporal differences were analyzed under the condition of promoting green innovation development. The results showed that the overall energy efficiency of the tertiary industry in the Yangtze River Economic Belt fluctuated greatly, and the spatial difference showed a decreasing trend; the energy efficiency in the downstream area(Jiangsu Province, Shanghai City and Zhejiang Province) was outstanding, the efficiency value was improved and the spatial difference was reduced; the spatial difference in the midstream area(Hubei Province, Hunan Province, Anhui Province and Jiangxi Province) decreased, but the overall efficiency value was low, and the efficiency level in the upstream area(Yunan Province, Guizhou Province, Chongqing City and Sichuan Province) was high, but the spatial difference expanded. There was still room for improvement in energy efficiency in 11 provinces and cities. Attention should be paid to energy conservation. There were great differences in efficiency changes among provinces and cities, so it was necessary to explore the path of efficiency improvement according to local conditions.
    Measurement of the level of ecology-economy-society coordinated development in resource-based cities:Taking Yan'an City, Shaanxi Province as an example
    LI Li, MENG De-xing
    2023, 62(5):  196-202.  doi:10.14088/j.cnki.issn0439-8114.2023.05.034
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    Taking Yan'an City as an example, an evaluation system covering ecology-economy-society was constructed, and the entropy weight method, coupling analysis method and obstacle degree analysis method were used to study the coupled development level of the ecology-economy-society system and the main influencing factors in Yan'an City from 2012 to 2021. The results showed that the developmental level of ecology, economy and society in Yan'an City had been continuously improved; the level of coupling of the ecology-economy-society system in Yan'an City was high, but the level of coordination was low and the development was slow; the five basic indicators of annual precipitation, per capita park green space, comprehensive utilization rate of general industrial solid waste, proportion of social fixed asset investment to GDP, and social fixed asset investment were the main constraining factors. Some suggestions were made from three aspects of ecological civilization construction, economic structure transformation and strengthening top-level design to promote the coordinated and efficient development in Yan'an City.
    Evolutionary game between agricultural retailers and consumers under the blockchain technology
    LIU Yan-feng, ZHENG Ke
    2023, 62(5):  203-208.  doi:10.14088/j.cnki.issn0439-8114.2023.05.035
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    In order to enhance the flow availability of agricultural supply and advance the quality of agricultural products, the evolutionary game model between consumers and agricultural retailers under government policy support was established. The equalization point between the two game models was found. And in this model, the system dynamics simulation technology was used to simulate. The key factors of agricultural retailers the adopt blockchain technology had been discovered, including strengthening the government's promotion of blockchain technology, increasing the cultivation of blockchain and supply chain technical talents and attracting interdisciplinary talents who master blockchain technology and other technologies, increasing policy support, and developing information technology related to blockchain, etc.
    Discussion on the development path of “water conservancy + tourism” in Shiquan County, Shaanxi Province from the perspective of global tourism
    YAN Zi
    2023, 62(5):  209-213.  doi:10.14088/j.cnki.issn0439-8114.2023.05.036
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    Under the background of “tourism +” strategy promoting industrial integration development and global tourism development, there were some problems in the development of “water conservancy + tourism” in Shiquan County, such as short life cycle of tourism destination, insufficient capital investment, failure to form brand effect of tourism destination and so on. According to the tourism destination life cycle theory, the development path mode of Shiquan County in different life cycle development stages of “water conservancy + tourism” was put forward, and combined with the geographical location, existing tourism resources and industrial development of Shiquan County, it was suggested that Shiquan County could fully grasp the tourism destination life cycle, give full play to the multiplier effect of “water conservancy + tourism” mode and develop the integrated development model based on “tourism +” strategy, promote Shiquan County to launch the brand of “water conservancy + tourism”, promote industrial integration, lengthen the regional tourism industry chain and promote rural revitalization.
    Evaluation and obstacle factors of water environmental governance performance in the Yangtze River Economic Belt based on DPSIRM model
    LI Qin
    2023, 62(5):  214-220.  doi:10.14088/j.cnki.issn0439-8114.2023.05.037
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    Based on the DPSIRM causal framework model, the water environment governance performance evaluation system was constructed. Using the entropy weight method, and combined with the linear weighting method and obstacle degree model, the comprehensive index of water environment governance performance in the Yangtze River Economic Belt from 2010 to 2019 was comprehensively evaluated and the main obstacle factors were identified. The results showed that, on the whole, the comprehensive index of water environment governance performance in the Yangtze River Economic Belt was rising rapidly. From the perspective of space, the overall water environment governance performance in the upstream and downstream regions was better, while the middle reaches were the worst and fluctuate greatly. The development trend of the performance index of each subsystem was different obviously, but all of them showed the trend of fluctuation and rise. From the comprehensive analysis of the main obstacle factors, the amount of fertilizer applied per unit cultivated land area, the growth rate of per capita disposable income of urban residents, the COD emission of ten thousand yuan industrial output value, the ammonia nitrogen emission from ten thousands yuan industrial production value and treatment capacity of industrial wastewater treatment facilities were the main obstacles which hindered the improvement of the water environment governance performance.
    Research on the competitiveness of vegetable industry in Yunnan Province
    LI Liang, CHEN Liang-zheng, DONG Xiao-bo, MAO Zhao-qing
    2023, 62(5):  221-226.  doi:10.14088/j.cnki.issn0439-8114.2023.05.038
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    Based on the development status and reality of Yunnan Province vegetable industry, the advantage index of vegetable industry in Yunnan Province was analyzed based on the regional scale advantage index, efficiency comparative advantage index, per unit yield advantage index, and scale comparative advantage index. And adopting the SWOT analysis method, the competitive advantages, disadvantages, opportunities and risks of the development of the vegetable industry in Yunnan Province were comprehensively analyzed. Combining the status quo of the Yunnan Province vegetable industry and taking the existing problems as the guidance, it had pointedly proposed to cultivate a diversified agricultural business integration subject, expand the versatility of the vegetable industry, create an export-oriented vegetable industry, improve the construction of the cold chain logistics system, and play the role of scientific and technological support and protection to enhance the competitiveness of Yunnan's vegetable industry.
    Quality evaluation and regional difference analysis of new urbanization in the core area of Huaihai economic zone
    LIU Xin-ran, XI Yan-tao
    2023, 62(5):  227-232.  doi:10.14088/j.cnki.issn0439-8114.2023.05.039
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    According to the development characteristics and actual situation of new urbanization in the core area of Huaihai economic zone, the comprehensive evaluation index system for the quality of new urbanization was established from three aspects of urbanization development, urban-rural overall planning, and resources and environment. The entropy method, difference coefficient and GIS spatial analysis method were used to reveal the temporal and spatial evolution process of new urbanization quality level in the core area of Huaihai economic zone from 2010 to 2018. The results showed that the quality of new urbanization in the core area of Huaihai economic zone was on the rise as a whole, and the quality difference within the region was obvious. The urbanization quality level of Xuzhou City had always maintained a leading position and belonged to the high quality with medium speed development mode; Jining City, Zaozhuang City, Lianyungang City and Suqian City had medium quality level of urbanization, and the development modes were medium quality with low speed, medium quality with medium speed, medium quality with medium speed and medium quality with low speed, respectively; Shangqiu City, Huaibei City and Suzhou City had relatively low urbanization quality level, and their development modes were low quality with low speed, low quality with high speed and low quality with low speed, respectively.
    Rural Revitalization
    Research on the present development status and influencing factors of farmers' professional cooperative: Taking Jingzhou City of Hubei Province as an example
    CHEN Yun-lian
    2023, 62(5):  233-240.  doi:10.14088/j.cnki.issn0439-8114.2023.05.040
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    By questionnaire survey, the development status and influencing factors of farmers' professional cooperatives were analyzed in Jingzhou City, Hubei Province. The results showed that under the active guidance and support of Jingzhou government, farmers' professional cooperatives played an important role in driving local employment and promoting the development of local agricultural economy. The six variables of the educational background of management personnel, socialization degree, agricultural professional technical services, credit support, circulation channels of agricultural products, and internal management system of cooperatives had a significant impact on the sales revenue of local farmers' professional cooperatives. Based on this, it was proposed to optimize the fund source channels of cooperatives and guarantee the reasonable, effective and timely fund supply of cooperatives; strengthen the construction of the internal management system of farmers' specialized cooperatives; accelerate the construction of agricultural product quality supervision system, and promote agricultural standardized production; enhance the education of managers and strengthen the training of members' technical skills; wided drive, and form synergistic effect; deepen innovation, and explore new models and other feasible policies, to proride new solutions for the development of local farmers' professional cooperatives.
    Investigation and research on rural public cultural service facilities under the background of rural revitalization:Taking Urumqi county as an example
    LIU Zhi-you, WEI Ran, QU Xiu-wu, HAO Pan-pan
    2023, 62(5):  241-246.  doi:10.14088/j.cnki.issn0439-8114.2023.05.041
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    Through the field visit and questionnaire survey in Urumqi county, Xinjiang, with Kano model as the research basis, the impact of farmers' satisfaction was classified and prioritized, and combined with Better-Worse coefficient clustering statistical method, the population was divided into three categories of young, middle-aged and elderly, and the clustering situation and impact degree of 16 public cultural needs of different age groups in four demand types were analyzed and tested. The results showed that different age groups had different emphases on essential needs, expectant needs, charismatic needs and undifferentiated needs. Therefore, priority should be given to promoting essential needs for all age groups; secondly, expectant needs and charismatic needs should be continuously improved; finally, undifferentiated needs should be comprehensively coordinated.
    The impact of “three rights separation” reform of homestead on rural revitalization
    LIU Xin-rui, ZHAO Wei-chao
    2023, 62(5):  247-252.  doi:10.14088/j.cnki.issn0439-8114.2023.05.042
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    Based on the panel data of 33 pilot areas of the national homestead reform, the annual per capita disposable income of farmers was used as the explanatory variable, the DID model was used to compare the impact of the “three rights separation” reform of homestead on rural revitalization, and the results were tested for robustness and analyzed for heterogeneity. The results showed that the reform of the “three rights separation” of homestead had a positive effect on promoting rural revitalization, but due to the difference in regional economic level and the lag in policy implementation, the promotion effect in some areas was not significant.
    Study on the relationship between pension concern and quality of life among unmarried rural men under the marriage squeeze
    ZHANG Qun-lin, WANG Yuan-yuan, LI Zhi-bin
    2023, 62(5):  253-257.  doi:10.14088/j.cnki.issn0439-8114.2023.05.043
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    Using the data from the Survey of Rural Family Marriage Status, the relationship between the perceived marriage squeeze, quality of life and pension concern among unmarried rural men was analyzed. The study found that the perceived marriage squeeze had significant negative effects on the quality of life of unmarried rural men, but the perceived marriage squeeze had significant positive effects on the pension concern. However, the quality of life had significant negative effects on the pension concern of unmarried rural men. The mediated effect analytical results showed that the quality of life played a mediating role in the relationship between the perceived marriage squeeze and pension concern of unmarried rural men. That was to say, the quality of life could buffer the positive effects of perceived marriage squeeze on the pension concern of unmarried rural men.