HUBEI AGRICULTURAL SCIENCES

Research progress on plant accumulation and metabolism of selenium and enlightenment on the development of selenium-rich planting industry

LI Lin-ling, ZHANG Guo-ji, GUO Jie, XIAO Xian, CHENG Shui-yuan, CHENG Hua

2021, 60(6): 5-14. doi:10.14088/j.cnki.issn0439-8114.2021.06.001

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This research progress summarizes and analyzes the relevant literature on selenium absorption and metabolism by plants at home and abroad. On the basis of expounding the role of plants on the environmental selenium cycle metabolism, the research progress in the absorption, transportation, accumulation and metabolism of selenium by plants in recent years and its enlightenment to the development strategy of Se-rich planting industry are systematically analyzed. The conclusion is expected to provide theoretical guidance for the rational use of environmental selenium resource advantages, the full use of plant selenium bio-enhancement functions, and the improvement of the level of Se-rich agricultural industries.

Research progress in pharmacological effects and clinical applications of Panax notoginseng saponins

LIANG Xiao-lian, LIU Qian-qian, LI Wen-li, CHEN Yong, HUANG Zhou-yan, LIU Wen-jun

2021, 60(6): 15-19. doi:10.14088/j.cnki.issn0439-8114.2021.06.002

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Panax notoginseng saponins are the main active components of Panax notoginseng, which have pharmacological effects such as anti-inflammation, reducing blood lipid, anti-oxidation and anti-tumor. Based on the review of domestic and foreign literature in recent years, the pharmacological effects and clinical application of Panax notoginseng saponins were summarized,so as to provide reference for the further research and application.

Effects of different density on grouting characteristics of maize Xinzhongyu 801 and Jingnongke 728

SHU Zhong-bing, LI Hui, DUAN Ming-yu, SONG Cheng-xiao, CHEN Lang, BI Shi-min

2021, 60(6): 20-22. doi:10.14088/j.cnki.issn0439-8114.2021.06.003

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Thegrouting characteristics of two hybrid maize varieties, Xinzhongyu 801 and Jingnongke 728, were studied by split plot design. The results showed that Xinzhongyu 801 was a big ear type variety with sparse planting, and Jingnongke 728 was a dense-tolerant type variety, through the analysis of the fresh weight of singlespike. The analysis of 100-grain fresh weight and dry weight showed that the grain size of maize was related to the heredity of varieties, but not influenced by planting density. Through the analysis of grain water content, it was found that the grain water content decreased gradually during the whole filling process. Through the analysis of the grain filling rate, it was found that the grain filling rateof Xinzhongyu 801 was also affected by planting density, but the grain filling rateof Jingnongke 728 was less affected by planting density. The fresh weight, dry weight and water content of 100-grain were related to the heredity of the variety, and were not affected by the planting density.

Effects of different water and fertilizer treatments on soil nitrogen and phosphorus fertility characteristics in rice-wheat rotation field

SUN Wan-ru, SHEN Jian-lin, LI Yong, WANG Yi, WANG Bo

2021, 60(6): 23-26. doi:10.14088/j.cnki.issn0439-8114.2021.06.004

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Different irrigation methods, types of nitrogen fertilizer, and phosphorus fertilizer levels were used to explore changes in nitrogen and phosphorus fertility of rice-wheat rotation fields in Taihu lake basin. The results showed that the total nitrogen, ammonium nitrogen, nitrate nitrogen, total phosphorus, and available phosphorus content in the soil after the rice harvest decreased by 3.73~12.27, 18.58~43.34, 7.36~24.15, 1.22~9.67 and 14.03~35.92 percentage points respectively, compared with those before soaking; Compared with traditional irrigation, intermittent irrigation treatments reduced the ammonium nitrogen and nitrate nitrogen of the soil after harvest by 12.81~20.25 and 2.46~14.36 percentage points, and the total phosphorus increased by 1.65~3.99 percentage points; In traditional irrigation and intermittent irrigation, the content of total nitrogen and total phosphorus in the soil after harvest treated with the new slow-release nitrogen fertilizer reduced by 30% was higher than that of conventional urea split fertilization and no fertilization treatments; Compared with the control treatment, the rice season with no phosphorus treatments after harvest, the total phosphorus in the soil increased by 0.38~5.62 percentage points. Therefore, intermittent irrigation, slow-release nitrogen fertilizer with 30% nitrogen reduction and rice season phosphorus-free treatment can effectively reduce soil nitrogen and phosphorus losses, thereby reducing water pollution caused by farmland nitrogen and phosphorus loss.

Applied research on preparation of biotechnology fulvic acid by straw

HUANG Ming-hui, ZHANG Qian-ru, HAN Meng-qi, GAO Xi-long, LI Hua, LUO Xing

2021, 60(6): 27-30. doi:10.14088/j.cnki.issn0439-8114.2021.06.005

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Using peanut straw,wheat straw and peanut red clothing as raw materials, biotechnology fulvic acid was prepared by fermentation method, and the effect of biotechnology fulvic acid on plant growth was studied. The results showed that different sources of biotechnology fulvic acid had different effects on seed germination, and different concentrations of biotechnology fulvic acid from the same source had different effects on seed germination. Among them,the biotechnology fulvic acid 15 times solution of peanut straw source increased the germination rate by 15.00 percentage points and the root length by 7.1% compared with the blank, and seed germination and radicle growth were better.

Evaluation on soil nutrient characteristics for protective area of agro-product geographical indications：Taking the apple orchard in Yicheng county of Shanxi province as an example

YAO Jun-feng, WU Yan-yan

2021, 60(6): 31-35. doi:10.14088/j.cnki.issn0439-8114.2021.06.006

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Taking the Yicheng apple orchard as an example to discuss spatial distribution characteristics of soil fertility for protective area of agro-product geographical indications. 300 soil samples were collected from protective area. Twelve indicators of soil fertility and integrated fertility index can be evaluated and distributed by employing SPSS 22.0 and ArcGIS, including total N, available N, available P, available K, organic matter, available S, available Cu, available Zn, available Fe, available Mn, available B, available Mo. The coefficient of variation as follows： Available S（2.08%）<available Mn（6.51%）<available Cu（9.66%）<available Zn（10.00%）<available Fe（13.89%）< available B（15.93%）<available Mo（19.16%）<organic matter（26.87%）<total N（37.58%）<available N（40.92%）<available K（48.82%）<available P（110.39%）. The integrated fertility index had been divided into five levels, ranging from 0.23 to 0.72, which dominated by 3th level（from 0.35 to 0.40） and 4th level（from 0.30 to 0.35）, accounting for 35.12% and 28.43%, respectively.

Evaluation on soil quality of newly-established urban green space in Nanning city

HUANG Li-dan, HUANG Xu-guang, ZHAO Jian-wen, JIANG Li-fu, QIN Ling, YANG Si-xia, LU Yan-song, ZHONG Qiong-qiong, HUANG Ling-pu, HUO Xing

2021, 60(6): 36-41. doi:10.14088/j.cnki.issn0439-8114.2021.06.007

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The soil physiochemical properties, such as pH, organic matter and available nutrients were determined. And soil improvement of road greening in Nanning, comprehensive soil fertility was evaluated by the Nemorow grading method. The results indicated that the texture types mainly included loam and viscous loam soil. The soil pH was dominated by neutrality（pH 6.5~7.5）. The average of soil bulk density was 1.14 g/cm³, CEC （cation exchange capacity） was 12.72 cmol（+）/kg. Organic matter and available nitrogen were distributed in grades 1 to 4 with an average of 31.54 g/kg and 110.24 mg/kg. Available phosphorus was above grade 2 or higher （high level） with an average of 43.4 mg/kg. All the available potassium （mean 59.21 mg/kg） were at the level of grade 4 and below medium content. Nemerow comprehensive fertility index analysis showed that the soil fertility index of newly-built green land in Nanning was between 1.19~1.78, which was in the middle and upper level. It was suggested to pay attention to the supply of natural organic matter in greenbelt soil and promote the benign cycle of urban soil ecosystem.

Research on the risk of collapse and landslide in Wenchuan earthquake disaster area based on logistic regression model

YANG Wei-hua, YANG Zhi-qiang, ZHAO Jian-lin, LIANG Yong-qi

2021, 60(6): 42-48. doi:10.14088/j.cnki.issn0439-8114.2021.06.008

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22 counties in the Wenchuan earthquake disaster area were selected as the study area. Based on 6 007 collapse and landslides data and multi-source information, using GIS technology, seven factors including elevation, slope, aspect, fault, lithology, rainfall, and land use were selected as the influencing factors for the evaluation of collapse and landslide susceptibility. Then based on the logistic regression model, the susceptibility to collapse was evaluated, and a susceptibility zone map generated. The results showed that the distribution of historical collapse and landslide points fitted well with the zoning map of collapse and landslide susceptibility. Wenchuan county, Mao county, Beichuan county, Mianzhu city, Qingchuan county, and Pingwu county in this area were areas with high susceptibility to collapse and landslides. The ROC curve was used to evaluate the success rate and prediction rate of the model, and the AUC （Area under curve） of the training sample set was 0.84, and the AUC of the verification sample set was 0.79, indicating that the logistic regression model used in this paper had a high prediction accuracy.

Research on the micro-renewal of traditional village landscape based on PSPL investigation and spatial syntax：A case study of Weipo village, Mengjin county, Henan province

YANG Zi-meng, YANG Fang-rong, ZHAO Yun, GUO Ming-hao

2021, 60(6): 49-53. doi:10.14088/j.cnki.issn0439-8114.2021.06.009

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Taking the public space of the Weipo village, Mengjin county, Luoyang city as the research object, this paper adopts the method of PSPL investigation to investigate the distribution of population flow in the site and collects the real renewal intention of tourists and villagers. Using the method of axis analysis and horizon analysis in spatial syntax, the important nodes of spatial update are selected by Depthmap software from the scientific point of view, the micro-renewal research is made with point-band surface. It is suggested that the road guidance system should be improved on the road leading to Weizi Peony Garden in Weipo village and the folk performing Arts Square, Jiukong Kiln and Buddha Hall. On the axis with higher integration degree in the village, the protection facilities of shading from sun and rain, seats, street lamps and other related infrastructure are added to reflect the humanization of the space and improve the comfort when visiting.

Spatial and temporal characteristics of LUCC in main urban area of Chongqing city based on GIS

WANG Yuan-yuan

2021, 60(6): 54-59. doi:10.14088/j.cnki.issn0439-8114.2021.06.010

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Based on 1988, 2000 and 2010 TM image data, combined with remote sensing and GIS technology the Chongqing city urban area from 1988 to 2010 of land use and cover change （LUCC） characteristics and the status of land use were revealed, and the migration of city of center of gravity was studied. 22 years of land conversionwas analyzed by using markov chain model, the development trend of Chongqing’s main urban area in the next 40 years was predicted. The results showed that from 1988 to 2010, all kinds of land areas in the main urban area of Chongqing city were changed, urban construction area increased by 593.95 km², and forest and grassland area increased by 102.8 km², and water area decreased by 166.85 km², and cultivated land decreased by 427.97 km², and unused land decreased by 101.93 km². In the past 22 years, the government had vigorously developed urban areas, and also paid attention to soil and water conservation and afforestation. Markov predicted that Chongqing’s main urban areas will maintain this trend for a long time to come.

Investigation and analysis of resistance to powdery mildew of rubber tree germplasm resources

GUAN Xin, PENG Yan-lin, HU Yan-shi, ZENG Xia, TU Min

2021, 60(6): 60-63. doi:10.14088/j.cnki.issn0439-8114.2021.06.011

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Powdery mildew of rubber tree broke out in the spring of 2020. At the end of the epidemic period, the resistance of Amazonwild germplasm and Wickham cultivated germplasm to powdery mildew was investigated in the national rubber germplasm resource nursery. The results showed that a total of 4 788 germplasm were investigated, among which 18 germplasm were disease resistant, accounting for 0.38%, and all were Amazon wild germplasm. 146 medium resistant germplasm （3.05%）, 145 wild germplasm and 1 Wickham germplasm. 1 180 moderately susceptible germplasm （24.64%）; 2 557 susceptible germplasm, accounting for 53.40%; There were 887 highly susceptible germplasm （18.53%）.

Isolation, purification and molecular identification of the pathogen of rice blast

ZHAO Shi-ming, WANG Mei-ling, LYU Feng-xia

2021, 60(6): 64-66. doi:10.14088/j.cnki.issn0439-8114.2021.06.012

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The leaves of rice infected with rice blast disease were collectted from different rice cultivation areas. Pathogen isolation and purification culture were carried out by PDA medium, and the morphology of colony melanin and conidia were identified by preliminary screening. Mycelia DNA was extracted with kit, PCR amplification was performed using specific primers of Blast, and the amplified products were sequenced and Nucleotide Blast comparisons were performed. The results showed that the disease sample was taken six cultivation areas after the initial screening to determine 5 strains of secretion of melanin and observed its conidium, PCR product sequencing confirmed that the two sample sequence was the same, and the imperfect fungi of NCBI website door of grey pear spore （Pyricularia grisea） highly homologous, determine to obtain pure strains of rice blast.

Efficacy and safety evaluation of 15% metazosulfuron·penoxsulam dispersible oil suspending agent against annual weeds in transplant rice field

LIU Ying, SHI Xiu-fei, DU Xiao-ying, LI Jun-kai

2021, 60(6): 67-69. doi:10.14088/j.cnki.issn0439-8114.2021.06.013

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Aiming to confirm the control effects of 15% metazosulfuron·penoxsulam dispersible oil suspending agent（OD） against annual weeds and safety in transplant rice field, field pharmacodynamic experiments were carried out by the stem and leaf spray methods. The results showed that the recommended dosage of 15% metazosulfuron·penoxsulam OD in field were 67.50~112.50 g （a.i.）/hm². At 30 day after spraying treatment, the weeds control effects on quantity and on fresh weight were 98.39% and 98.83%, respectively. Within the range of recommended dose,15% metazosulfuron·penoxsulam OD could effectively control weeds in transplant paddy field, and it was safety to rice.

Analysis on main control factors of total plant alkaloid in upper leaf of flue-cured tobacco in Enshi tobacco area

DENG Jian-qiang, LIU Li-ping, WANG Hong-wei, ZHUANG Tao, DAI Hui, LU Cheng-nian, LIU Jun

2021, 60(6): 70-75. doi:10.14088/j.cnki.issn0439-8114.2021.06.014

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The effects of nitrogen application rate, transplanting density and soil chemical properties on total plant alkaloids of upper leaves of flue-cured tobacco were studied in a field experiment with the flue-cured tobacco plants planted in different soil fertility areas in Enshi. The results showed that, ① compared with transplanting density, nitrogen application rate had a greater effect on total alkaloid content, but increasing planting density was helpful to decrease total alkaloid content under medium and low nitrogen application rates;② covariance analysis showed that the effect of nitrogen application rate on total alkaloids was significant, but the interaction between nitrogen application rate and transplanting density was not significant;③ the total alkaloid was negatively correlated with soil available phosphorus, and the available phosphorus entered the stepwise regression model in 2018 and 2017, and controlled the total alkaloid;④ the path analysis method of structural equation model （SEM） showed that with the increase of available phosphorus, the total alkaloid content decreased significantly, and the indirect path coefficient of available phosphorus was significant;⑤ The indirect path coefficient of available P and available K was larger, accounting for about 80% of the intermediate path coefficient,the total alkaloid could be affected indirectly by available potassium. Proper control of the amount of nitrogen fertilizer and high-density planting measures were adopted in some areas where the total alkaloids were easy to be high. Soil available phosphorus could be one of the main controlling factors ofthe total alkaloid.With the gradual enrichment of soil phosphorus, it would be beneficial to reducethe total alkaloid , but the effects of other quality factors of tobacco leaves needs further investigation.

Surveying DNA epigenetic diversity in the foreign hot pepper germplasms

XU Xiao-wan, XIA Bi-bo, WU Zhi-ming, TIAN Yong-hong

2021, 60(6): 76-80. doi:10.14088/j.cnki.issn0439-8114.2021.06.015

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Nine pairs of primers with good repeatability and clear bands were screened from 63 pairs of MSAP primers, and 30 hot pepper （Capsicum spp.） germplasms introduced from abroad were amplified by MSAP. The analysis of DNA methylation pattern showed that fully methylation was major methylation type in 30 hot peppers. However,MSP（Methylation-sensitive polymorphisms） clustering and MISP（Methylation-insensitive polymorphisms） clustering were all relatively discrete, and the related principal coordinate analysis was basically consistent with the clustering analysis results. The DNA methylation study did not gather Capsicum spp. into the same group. It is shown that the epigenetic variation of hot pepper is more abundant than the genetic variation of the genome.

Study on planning of waterfront ecological corridor：A case study of Shuangqiaoxi park in Chongqing city

LIU Zi-yin, SUN Li-na, SUN Li-qiang, WANG Peng-fei

2021, 60(6): 81-85. doi:10.14088/j.cnki.issn0439-8114.2021.06.016

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Based on the actual demand of urbanization for ecological landscape, the application of waterfront ecological corridor was discussed, taking Shuangqiaoxi park in Chongqing as the research object, based on the concept of ecological restoration and protection andthe principle of ecological priority, the current situation and limiting factors of the base were analyzed. Through the planning and design strategy, through the ecological landscape restoration and protection, to build a benign and stable, diverse and sustainable ecological foundation, to create an ecological stability, rich landscape public interactive space. The design strategy of waterfront ecological corridor was summarized, in order to provide reference for waterfront landscape restoration.

Research on the transformation path of traditional orchards under the guidance of sightseeing agriculture：Taking the Shishan lake Yasen Agricultural Sightseeing park in Xinyang city as an example

WANG Meng-chen, YUAN De-qiang, WANG Peng-fei

2021, 60(6): 86-89. doi:10.14088/j.cnki.issn0439-8114.2021.06.017

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The study taked the Shishan lake Yasen Agricultural Sightseeing park in Xinyang city as an example, analyzed its development constraints and resource advantages based on field research data, grasped the integration principle of agricultural tourism and carried out the design of four sections of viewing experience, leisure and entertainment, scientific research and education and service management. After that, the overall planning layout of “one heart, one axis, two corridors, four districts and multiple points” was formed. The aim is to provide a reference for the development of agricultural garden in Xinyang city and other agriculture-oriented areas in Henan province.

The rapeutic effect of 8 kinds of aquaculture drugs on the Acrossocheilius hemispinus infected Ichthyopthirius multifillis

CHEN Bin

2021, 60(6): 90-94. doi:10.14088/j.cnki.issn0439-8114.2021.06.018

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In order to obtain the effective drugs for the treatment of Acrossocheilius hemispinus infected Ichthyopthirius multifillis, the therapeutic test of 8 aquaculture drugs with different density on the Acrossocheilius hemispinus artificial infected Ichthyopthirius multifillis was studied. The results showed that copper sulfate could accelerate the division and multiplication of Ichthyopthirius multifillis. Baicao antidote（natural plant carbon）had no effect on killing Ichthyopthirius multifillis vegetative body. When the formaldehyde solution was used alone, it had a certain killing effect on the vegetative body of Ichthyopthirius multifillis, but the treatment cycle was long and the survival rate of diseased fish was low. Benwenjing（dried ginger, pepper, apricot leaves） had no effect on killing the vegetative body of Ichthyopthirius multifillis, but it can improved the resistance of the diseased fish and reduced the mortality rate, so it should be used in conjunction with other insecticides. When the industrial salt（NaCl）concentration was between 3 000 and 5 000 mg/L, it could effectively remove the vegetative body of Ichthyopthirius multifillis off the diseased fish, and the cure rate of the diseased fish could reach （86.7±9.4）%. The Xiaoguajuesha（matrine, capsaicin）could accelerate the division and proliferation of Ichthyopthirius multifillis in the early stage, but it could effectively kill the larvae of Ichthyopthirius multifillis in the later stage. It was suitable to be used when the number of Ichthyopthirius multifillis was not large. When the treatment concentration of Yukangle（active calcium carbonate）was 4~6 mg/L, the insecticidal effect was obvious, and the cure rate of the diseased fish was（46.7±9.4）%~（60.0±0.0）%; When the treatment concentration of Bailingxiaoguajing（plant extracts）was 1.6~2.0 mg/L, the insecticidal effect was obvious, and the cure rate of the diseased fish was（80.0±0.0）%.

Acute toxic effects of four common disinfectants on Ruditapes philippinarum

CHEN Zhi, LI Wei-peng, HU Feng-xiao, HUANG Jian, CHEN Qi-chun, WEI Cheng-chen, LIAN Cheng-yang

2021, 60(6): 95-99. doi:10.14088/j.cnki.issn0439-8114.2021.06.019

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In order to obtain the safe concentration of four common disinfectants on the scallop of Ruditapes philippinarum, and provides a theoretical basis for antisepsis treatment of Ruditapes philippinarum before artificial oxytocin, in this study, the acute toxicity of potassium permanganate, povidone iodine, chlorine dioxide and formaldehyde to Ruditapes philippinarum was tested by static acute toxicity test. The result showed that the safe concentration of the four disinfectants was potassium permanganate （0.24 mg/L）,povidone iodine （11.04 mg/L）,chlorine dioxide （0.68 mg/L）,formaldehyde（1.87 mg/L）; it can be known that the four disinfectants to Ruditapes philippinarum was potassium permanganate > povidone iodine > chlorine dioxide > formaldehyde. Povidone iodine should be used for disinfection in the process of oxytocin breeding of Ruditapes philippinarum.

Study on community structure and diversity of zooplankton in Qingshui river

SHI Wei, DUAN Jie-reng, QIU Xiao-cong, WANG Xiao-yi

2021, 60(6): 100-104. doi:10.14088/j.cnki.issn0439-8114.2021.06.020

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The 22 sample sites of Qingshui river basin were surveyed in July （summer） and November （winter） 2018,the zooplankton species composition, density, biomass, common species,dominant species composition and seasonal variation were studied to explore the population structure and diversity of zooplankton in Qingshui river basin. The results showed that 23 species of zooplankton were identified in Qingshui river basin, including 8 species of rotavirus, 8 species of dendritic and 7 species of copepods. There were 6 kinds of dominant species, of which the daphnia was the most sharp. Zooplankton species, density, biomass and distribution varied by season, with summer species, densities and biomass peaking and winter at a low ebb. The Margalef index of zooplankton in Qingshui river basin was 0.432~0.997, Shannon-Wieaver index was 1.251~1.922, and evenness index was 0.233~2.066. The structure and diversity of zooplankton population were different in different sampling sites. According to Margalef index, Shannon-Wieaver index and evenness index, it can be preliminarily judged that water quality in Qingshui river basin is seriously polluted and moderately eutrophic, which needs to be strengthened for treatment and protection.

Night distribution pattern of bats species diversity in Xishuangbanna

CAO Hui, YANG Jian-bo

2021, 60(6): 105-107. doi:10.14088/j.cnki.issn0439-8114.2021.06.021

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In order to explore the night variation of bat species diversity in Xishuangbanna, acoustic monitoring detector was used to probe bat activities, analyzed the variation of bat species diversity in different time at night during a year. The results showed that the distribution of bat species diversity at night was significantly different, except 18：00-20：00 and 20：00-22：00, 22：00-00：00 and 00：00-02：00, 00：00-02：00 and 02：00-04：00. In the seasonal variation, the first peak of bat species diversity in autumn and winter is one hour earlier than that in spring and summer.

Distribution and activity of serum protein of pregnancy and non-pregnancy Erinaceus amurensis Schrenk

JIN Zhi-min, JIA Xiu-qi, YANG Xin-yu, SU Hang

2021, 60(6): 108-109. doi:10.14088/j.cnki.issn0439-8114.2021.06.022

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The discontinuous polyacrylamide gel electrophoresis was used to analyze and compare the activities of serum proteinof pregnancy and non-pregnancy Erinaceus amurensis Schrenk. The results showed that the non-pregnant Erinaceus amurensis Schrenk serum protein electrophoresis separated 20 bands with a mobility of 0.009~0.590,pregnant Erinaceus amurensis Schrenk serum protein electrophoresis separated 25 bands with a mobility of 0.005~0.590. There were significant differences in the distribution, activity and number of electrophoretic bands between pregnant and non-pregnant individuals.

Study on optimization of extraction process of Bupleurum chinensis saponins by central composite design-response surface methodology

ZHAO Yan-dan, ZHAO Yan-jie, LI Xiao-yang, SONG Li-hua

2021, 60(6): 110-114. doi:10.14088/j.cnki.issn0439-8114.2021.06.023

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On the basis of single factor experiment, the effect of extraction times on the content of saponins in Bupleurum chinense was analyzed. The extraction process of saikosaponins was optimized by response surface method of central composite design. The effects of extraction time, extraction temperature and the ratio of liquid to material on saikosaponins were investigated. The results showed that the best conditions of total saponins of Bupleurum chinense were extraction temperature 80 ℃, extraction time 60 min, liquid-to-material ratio 8∶1（mL∶g）, and extraction twice. Under the optimal condition, the content of total saponins of Bupleurum chinense was 1.80 mg/g, which was well fitting with the predicted value of 1.81 mg/g. The extraction process by response surface methodology is accurate, reliable and predictable.

Design of water fertilizer integrated irrigation system based on PLC

CHAO Yang, LI Pei-dong, HUANG Lin-gang

2021, 60(6): 115-118. doi:10.14088/j.cnki.issn0439-8114.2021.06.024

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A set of water fertilizer integrated irrigation controller with high stability, simple operation, low cost and sufficient function was studied and designed. The system used sensor technology, micro-computer technology, PLC as the core, and drip irrigation technology, according to the soil humidity and the required fertilizer and water threshold in the process of crop growth, to carry out automatic irrigation and fertilization. The results showed that the system could effectively improve the productivity of facility agriculture and the efficiency of fertilizer and water use, save the amount of water resources, and provide some technical support for small and medium-sized facility agriculture farmers in western region.

An hourly prediction model of air temperature based on deep GRU neural network

LUO Yu, LUO Lin-yan, FAN Jia-zhi, DUAN Si-ru, GAO Wen-juan

2021, 60(6): 119-122. doi:10.14088/j.cnki.issn0439-8114.2021.06.025

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Base on deep gated recurrent unit （GRU） neural network, an hourly prediction model of air temperature in Shimen county are proposed by using hourly surface meteorological data （air temperature, air pressure, dew point temperature, relative humidity and water vapor pressure）, besides the accuracy of the model is analyzed. As the result indicated, the accuracy of the deep GRU model gradually declines with the prediction horizons, whose coefficients of determination, mean absolute error, mean values of root square error and the prediction accuracy are 0.996~0.906, 0.359~1.974, 0.510~2.562 ℃ and 62.529% respectively, which has higher performance than the prediction model based on autoregressive integrated moving average （ARIMA）, and reflects turning changes of air temperature as well. The hourly air temperature prediction accuracies in Shimen from April to July in 2019 are contrasted of the deep GRU model and numerical weather predictions （NWPs） from European Center in addition to Japan. The result shows that the deep GRU model would be a beneficial supplement to the air temperature short-impending prediction within 12 hours due to its prediction accuracies are all better than NWPs in 3, 6 and 9 h horizons.

The reform of agricultural scientific research evaluation system in China

SI Zhi-zhi, LI Jin-hu, GUO Jing-li

2021, 60(6): 123-126. doi:10.14088/j.cnki.issn0439-8114.2021.06.026

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The agricultural research evaluation system is one of the important basis and tool for the evaluation of agricultural scientific research. However, the evaluation system of agricultural research adopted in China is imperfect,which has some problems in practice. Based on the experience of foreign agricultural scientific research evaluation system, this paper considered the reform ideas of China's agricultural scientific research evaluation system, and proposed to establish a correct value orientation and build a “four in one” agricultural scientific research evaluation system.

The current situation and protection of agro-cultural heritage of species in Hubei province

WANG Ran, LEI Shu-yan

2021, 60(6): 127-130. doi:10.14088/j.cnki.issn0439-8114.2021.06.027

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Based on the investigation and research on the agro-cultural heritage of the species in Hubei province, The results showed that the protection of the ago-cultural heritage of the species was low, the management was lack, and the cultural connotation was lacking. Therefore, it was necessary to strengthen the management and protection, realize the dual protection of the material cultural heritage and the intangible cultural heritage, and promote the integrated development of culture, agriculture and tourism.

Analysis of tourism resources evaluation model based on BP neural network

LIU Yan-wei

2021, 60(6): 131-134. doi:10.14088/j.cnki.issn0439-8114.2021.06.028

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At present, the evaluation of tourism resources had a strong subjective color. There were many variables and strong nonlinearity in each evaluation model. BP neural network model used MATLAB calculation program to evaluate tourism resources, which can avoid the influence of subjective factors on the evaluation results. By using the methods of literature comparison, theoretical analysis and expert consultation, the evaluation index system including 7 first-class indexes and 16 second-class indexes was constructed. On this basis, the evaluation model was designed, and the error value in the evaluation model was analyzed emphatically. The evaluation model was applied to the tourism resources evaluation of several major cities, and the evaluation results and error range meet the requirements.

Spatial pattern analysis of strategic emerging industries in Jiangsu province

WANG Yan-yan

2021, 60(6): 135-138. doi:10.14088/j.cnki.issn0439-8114.2021.06.029

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Taking 109 districts and counties in Jiangsu province as the spatial analysis units, the spatial agglomeration pattern of strategic emerging industries in Jiangsu province in 2017 was studied by using spatial autocorrelation method. The results showed that, there were large spatial differences in the overall spatial distribution of strategic emerging industries in Jiangsu province, which was more in southern Jiangsu and less in central Jiangsu and northern Jiangsu. There was an obvious spatial agglomeration pattern,which were mainly high-high-level agglomeration and low-low-level agglomeration, high-high-level agglomeration was mainly concentrated in Suzhou-Wuxi-Changzhou region, and low-low-level agglomeration was mainly concentrated in northern Jiangsu. The results provided a reference for the government to formulate the strategic emerging industrial policies of different districts and counties, and provided the basis for the better development of strategic emerging industries.

The dialectical relationship between the theory of “Two Mountains”and the development of outdoor sports resources

YOU Mao-lin, LIU Jun

2021, 60(6): 139-142. doi:10.14088/j.cnki.issn0439-8114.2021.06.030

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Since 2016, the central government had issued a number of policies to support the development of outdoor sports. At the same time, more and more people participated in outdoor sports. Outdoor sports had been placed in the hope of targeted poverty alleviation and rural revitalization in many places, which had pushed the development of China’s outdoor sports resources into the fast track. Followed by the construction of outdoor sports infrastructure and the influx of outdoor sports enthusiasts into recreation areas, it will inevitably threaten the local ecological environment security. Under the guidance of the “Two Mountains” theory, outdoor sports resources should be properly developed and utilized, but the ecological environment should not be sacrificed. It was necessary to protect the ecological environment as the basis for the sustainable development of human society. Therefore, the development of outdoor sports resources should make full use of the high-quality natural conditions of the original ecology, guard against over industrialization, and avoid the environmental impact caused by large-scale construction and the influx of a large number of external population, the strategy of protecting outdoor sports resources should be adopted.

Construction and evaluation of evaluation index system of ecological civilization construction level in southern Shaanxi

DUAN Kai, LI Rong-rong

2021, 60(6): 143-146. doi:10.14088/j.cnki.issn0439-8114.2021.06.031

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Ecological problem is a topic of great concern to human beings, and it is an important and urgent strategic task for national development. How to objectively and accurately evaluate the level of ecological civilization construction and make scientific and correct planning, we need to establish a scientific and effective evaluation index system of ecological civilization construction level. Based on the relevant theories of ecological civilization, this study taking three cities in southern Shaanxi （Shangluo city, Hanzhong city and Ankang city） as the research objects, the evaluation index system of ecological civilization construction level applicable to the three cities in southern Shaanxi was constructed by analytic hierarchy process（AHP） method, and the ecological civilization construction level of the three cities in southern Shaanxi from 2016 to 2018 was evaluated, and corresponding improvement measures were proposed.

Research on the questions of order vegetables in Shiyan city

WANG Wei, WU Xun-shan, LAN Yu-mei, GONG Shi-fei, GUO Yuan-ping

2021, 60(6): 147-150. doi:10.14088/j.cnki.issn0439-8114.2021.06.032

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Shiyan city was located in the core water source area of the central route of South-to-North Water Diversion Project and the deep poverty-stricken of Qinba mountain. The development of order vegetables by the way of production and marketing docking can effectively realize the optimal of resources and the promotion of economic benefits. This study introduced the development status of the city’s order vegetables and analyzed the main problems in the production of the city’s order vegetables from the perspectives of government, order enterprises and farmers. Combined with the problems, this study put forward countermeasures and suggestions from three aspects, such as the government increasing industrial support, the order enterprises’ accelerating the construction of their own organizational capacity, and the farmers’ enhancing the awareness of production and marketing docking.

Spatiotemporal characteristics of coordinated development of population-resources-environment in Jiangsu province

WANG Qing-jun, DU Xiao

2021, 60(6): 151-156. doi:10.14088/j.cnki.issn0439-8114.2021.06.033

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Through the construction of the evaluation index system of the coordinated development of population-resources-environment in Jiangsu province, the weight of the index was determined by the vertical and horizontal grading method. Combined with the calculation model of coordinated development degree, the coordinated development degree of population-resources-environment system in Jiangsu province in 2010, 2015 and 2017 were calculated, and its spatial and temporal characteristics were analyzed. The results showed that the comprehensive evaluation score and coordinated development degree of population-resources-environment system of Jiangsu province and provincial cities were on the rise, but there was still room for improvement; in addition, the coordinated development degree of population-resources-environment system of Jiangsu province had obvious regional differentiation, showing a distribution pattern of high in the south and low in the north.

Research on the satisfaction of housing resettlement of rural disaster immigrants： Taking the cyclone immigrants in Sheyang county as an example

FENG Xuan-feng, LI Yi

2021, 60(6): 157-160. doi:10.14088/j.cnki.issn0439-8114.2021.06.034

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Taking the cyclone immigrants in Sheyang county as the research object,based on the questionnaire survey and field investigation, this study analyzed the satisfaction degree of housing resettlement from three aspects： Housing resettlement policy, housing conditions and living environment. The results showed that the housing resettlement satisfaction of centralized resettlement was much higher than that of decentralized resettlement, mainly due to the influence of housing conditions, living environment and other factors; affected by the housing resettlement policy, there were significant differences in the housing resettlement satisfaction of different resettlement sites. On this basis, the study put forward relevant suggestions, in order to find ways to improve the satisfaction of disaster immigrants housing resettlement.

Performance evaluation of village assistance in Awati county of Xinjiang based on analytic hierarchy process

ZHANG Wan-qing, WANG Hua-li

2021, 60(6): 161-165. doi:10.14088/j.cnki.issn0439-8114.2021.06.035

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According to the field investigation, this study established a performance evaluation index system of village assistance in Awati county of Xinjiang, including 5 first level indicators and 24 second level indicators, and constructed a hierarchical structure model. By using the analytic hierarchy process, it was concluded that the weight of industrial assistance and employment assistance was the highest, which was the main factor to measure the performance of village assistance in Awati county of Xinjiang. Finally, the study put forward relevant suggestions from five aspects： Strengthening the implementation of policies, optimizing the accurate identification work, strengthening the joint force between industry and employment, maintaining the sustainability of the effect of village assistance, and improving the quality of the cadre team of village assistance.

The system construction of French environmental basic database and its enlightenment to China

FEI Meng-yan

2021, 60(6): 166-170. doi:10.14088/j.cnki.issn0439-8114.2021.06.036

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Big data has been applied and developed rapidly in the process of promoting the modernization of national ecological environment governance system and governance capacity. The construction of environmental basic database has become one of the six main directions in the construction of digital China. France has specially stipulated the environmental basic database system in the form of legislation, including the basic framework, establishment and network services, information disclosure and sharing mechanism, which enlightened China to establish the unified environmental basic information standard, refer to the special competent department, promote cooperation with third-party commercial institutions and data resource sharing, and build open public services platform.

Research on the incentive mechanism of village cadres under incomplete contract

WANG Ping

2021, 60(6): 171-175. doi:10.14088/j.cnki.issn0439-8114.2021.06.037

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This research introduced the game theory of economics into the study of incentive mechanism of village cadres, using the principal-agent theory as the theoretical tools and logic support, using incomplete contract as the core concept and research condition, analyzing the behavior logic of village cadres, to reshape the incentive system of village cadres as the main line, put forward the countermeasures and suggestion to avoid the deviation of policy implementation in rural governance.

Impactions of economic policy uncertainty and swine fever on pork price

ZHANG Lian, YUAN Yong-sheng

2021, 60(6): 176-179. doi:10.14088/j.cnki.issn0439-8114.2021.06.038

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On the basis of relevant research, this study focused on the comprehensive impact of economic policy uncertainty and swine fever on pork price, and made an empirical analysis on China’s pork price from 2009 to 2019 by using deterministic factor decomposition and logarithmic multiple regression model. The results showed that, China’s pork demand showing a seasonal cyclical change, which had a seasonal impact on pork price; the economic policy uncertainty and swine fever will increase the supply gap and impact on pork price.

Characteristic analysis and prospect of selenium and tourism integration in Enshi prefecture

WANG Shi-ling, CHEN Pu-liang

2021, 60(6): 180-183. doi:10.14088/j.cnki.issn0439-8114.2021.06.039

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Taking Enshi prefecture as a sample, this study introduced the resource endowment and industry development status of Enshi prefecture from two dimensions of selenium and tourism, the theoretical basis of selenium and tourism integration and its application in Enshi prefecture were expounded. On this basis, further suggestions were put forward to promote the integration of selenium and tourism, promote the development of local economy, create characteristic growth pole in Enshi prefecture.

Research on the development of China's national central cities

ZONG Ya-fei, CAO Yue-e, GU Yun-zhi

2021, 60(6): 184-188. doi:10.14088/j.cnki.issn0439-8114.2021.06.040

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National central cities were not only the leaders of the whole country’s economy development, but also an important support point for participating in global economy trade and cooperative division of labor. The establishment of national central cities can promote the overall development of regional economy and realize the integration of regional economy development. Taking 16 cities as the research objects, this study analyzed the advantage dimensions of each city in the development status under the six dimension index system. The results showed that Beijing, Shanghai, Guangzhou and Chongqing were the national central cities in the four directions of north, east, south and west, and their leading position in economy development was obvious. The economy agglomeration degree of national central cities was obvious, but the effectiveness of environmental governance need to be improved, and scientific and technological innovation need to be strengthened.

Research on the high quality development path of global tourism under the background of culture and tourism integration：Taking Nanxun district of Huzhou city as an example

XIONG You-ping

2021, 60(6): 189-192. doi:10.14088/j.cnki.issn0439-8114.2021.06.041

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Through the cross-border and cross-regional allocation and integration of culture and tourism resources and elements, promoting the comprehensive optimization and improvement of tourism destination's functional efficiency and functional types was the key and difficult point of global tourism. Nanxun district of Huzhou city had gradually improved the global tourism innovation platform, the whole industry chain, and the brand effect. It had a good practical foundation and first-mover advantages. It was necessary to update the concept of global tourism development, optimize the functional pattern of global tourism, develop the characteristic products of global tourism, and optimize the operation environment of global tourism, so as to realize the high quality development of global tourism under the background of culture and tourism integration.

Research on satisfaction of rural tourists in Shanshan county based on perceived value

GAO Ya, YANG Li-min, YANG Hai-bin

2021, 60(6): 193-200. doi:10.14088/j.cnki.issn0439-8114.2021.06.042

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On the basis of combing the relevant literature, based on the analysis of the relevant data of rural tourism tourists in Shanshan county, a structural equation model of the perceived value of rural tourism, tourist satisfaction and tourist loyalty is constructed, and an empirical study is carried out. The results show that environmental perception, infrastructure perception, and public service perception in rural tourism in Shanshan county have a significant or extremely significant positive impact on tourist satisfaction, and tourist satisfaction also has a significant positive impact on tourist loyalty. Tourists' perceived value indirectly positively affects loyalty through satisfaction. The influence path of each variable is not the same. Based on this, starting from the perceived value of rural tourism, suggestions are made for the development of rural tourism in Shanshan county.

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