Molecular detection and analysis of main quality related genes in wheat
SUN Hua-wei, LING Dong, ZHANG Dao-rong, TANG Qing, ZHOU Fang-ju, TANG Qing-yi, LIU Xian-bin, WANG Zhi-shun, TANG San-ming, REN Xi-feng
2022, 61(20):
216-220.
doi:10.14088/j.cnki.issn0439-8114.2022.20.040
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In order to clarify the distribution and frequency of related quality genes in 88 parental materials and provide parental materials for wheat quality breeding, molecular markers were used to detect the genes related to quality including Ax2*, AxN, Bx7, By8, Dx5 genes, polyphenol oxidase genes and 1B/1R translocation. The results showed that the distribution frequencies of Ax2*, AxN, Bx7, By8 and Dx5 genes were 4.55%, 57.95%, 92.05%, 35.23% and 43.18%, respectively, and the frequency of 1B/1R translocation was 21.59%. The frequencies of polyphenol oxidase genes PPO-A1a (high PPO), PPO-A1b(low PPO), PPO-D1a (low PPO) and PPO-D1b (high PPO) were 45.45%, 54.55%, 55.68% and 44.32%, respectively. The frequency of genotype PPO-A1b/PPO-D1a (double low PPO) was 32.95%, and the frequencies of genotype PPO-A1a/PPO-D1a (medium PPO) and PPO-A1b/PPO-D1b (medium PPO) were 22.73% and 21.59%, respectively. The frequency of genotype PPO-A1a/PPO-D1b (double high PPO) was 22.73%. There were few parent materials polymering multiple high-quality genes. There were 3 materials containing 7+8,5+10 subunits, non-1B /1R translocation and double low PPO activity (ppo-A1b/ppo-D1a), which were Zhengmai 119, Zhengmai 578 and Gaoyou 5766, respectively, and could be used as parent materials for breeding high-quality wheat.