Abstract: Through the initiating and proliferating culture of Rhododendron sinogrande seedling, the sterilization method and media components of initiating and proliferating culture suitable for R. sinogrande were explored to establish the tissue culture system of R. sinogrande. The results showed that, the fungus contamination rate of two kinds of Rhododendron were lower when they were sterilized for eight minutes under 0.1% HgCl₂, and the fungus contamination rate for R. sinogrande and R. edgeworthii Hook. f. was 5% and 10%, respectively. The initiation rate of R. sinogrande seeding was the highest for 80%, when the concentration of IBA was 0.3 mg/L and the concentration of ZT was 0.01 mg/L in the medium; The multiple proliferation was the highest for 5.32, when the concentration of TDZ was 6 mg/L in the medium.

Key words: Rhododendron sinogrande Balf. f. et W. W. Sm., tissue culture, auxin, cytokinin