HUBEI AGRICULTURAL SCIENCES ›› 2019, Vol. 58 ›› Issue (9): 114-116.doi: 10.14088/j.cnki.issn0439-8114.2019.09.027

• Detection Analysis • Previous Articles     Next Articles

Quantitative determination of 4 isoflavones of Pueraria lobata and Pueraria thomsonii from Zitong in Sichuan province by HPLC

XU Wei-feng1, DU Chun-yan1, XU Shu-yu1, LI Qi-yuan1, SU Li-li1, YANG Gang2, JIANG He-zhong1   

  1. 1.School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;
    2.Zitong Professional Cooperative of Fuku Gegen/Zitong Tianfu Biotechnology Co.,Ltd.,Zitong 622150,Sichuan,China
  • Received:2018-10-23 Online:2019-05-10 Published:2019-12-05

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Abstract: To determine the contents of four isoflavones of Pueraria lobata and Pueraria thomsonii in Sichuan Zitong by HPLC. The chromatographic conditions were YMC-Pack ODS-A C18 column（250 mm×4.6 mm,5 μm）. The linear range of puerarin, 3′-methoxy puerarin, daidzin and daidzein were 0.050~0.750, 0.010~0.150, 0.010~0.150 and 0.012~0.180 μg, respectively, and it showed a good linear relationship（r>0.999）, the average recovery rate was between 96.52%~100.22%, and RSD was between 0.75%~1.84%（n=6）. This method is simple, accurate and reproducible. It is suitable for the determination of isoflavones in Puerarin. The contents of 4 isoflavones of P. lobata are obviously higher than P. thomsonii from Zitong, Sichuan Province. And the content of daidzein in P. lobata and P. thomsonii from Zitong is obviously higher than other habitat origins reported.

Key words: Pueraria lobata, Pueraria thomsonii, HPLC, isoflavone, content

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