Improving the thermotolerance of mannanase through molecular improvement

doi:10.14088/j.cnki.issn0439-8114.2023.12.027

HUBEI AGRICULTURAL SCIENCES ›› 2023, Vol. 62 ›› Issue (12): 151-153,162.doi: 10.14088/j.cnki.issn0439-8114.2023.12.027

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Improving the thermotolerance of mannanase through molecular improvement

WANG Ya-wei¹, XUE Qiang², XIONG Hai-rong²

1. School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430048,China;
2. College of Life Sciences, South-Central Minzu University, Wuhan 430074,China

Received:2023-09-25 Published:2024-01-10

Abstract

Abstract: The mannanase gene （KJ806638） from Thermobifida fusca was used as the research object, and it was modified by the error-prone PCR method, and the heat resistance of mannanase was further improved. The optimum reaction temperature of N16I mutant was increased from 72.5 ℃ to 77.5 ℃. Differential scanning fluorometry （DSF） testing results showed that the apparent decomposition temperature T_m of the enzyme protein increased from 61.9 ℃ to 63.2 ℃.

Key words: mannanase, error-prone PCR, thermostabilty

CLC Number:

WANG Ya-wei, XUE Qiang, XIONG Hai-rong. Improving the thermotolerance of mannanase through molecular improvement[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 151-153,162.

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Improving the thermotolerance of mannanase through molecular improvement

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