Establishment of LPS-induced rabbit fever model and evaluation of antipyretic effects of Zhuang and Yao medicine ingredients

doi:10.14088/j.cnki.issn0439-8114.2023.12.022

Abstract

Abstract: The optimal dose of replicating the fever model of endotoxin （LPS） in rabbits was evaluated using different doses of LPS intravenously. 1 h after modeling, all extracts and active monomers of Zhuang and Yao ethnic medicine were administered. The rectal temperature changes of LPS rabbits were measured by the intelligent pyrogen instrument, and the anal temperature growth value, change rate, antipyretic score at each time point and the total antipyretic score of each test sample were calculated. The results showed that after LPS injection for 1 h, the temperature of all rabbits increased by 0.8~3.0 ℃ compared with the basal rectal temperature, and the temperature of the model rabbits remained high after molding, and there was no significant change. Compared with the model group, the rectal temperature change rate of rabbits treated with 7 kinds of extracts from Zhuang and Yao ethnic medicine was significantly reduced in 1 or more time points, and the order of the total heat antipyretic score was as follows： Ethanolic extract of Christia vespertilionis （L. f.） Bahn. f.>water extract of Lindernia ruellioides （Colsm.） Pennell.>water extract of Gynura divaricata （Linn.）DC. >ethanolic extract of Polygonum chinense L.>petroleum ether extract of Lindernia ruellioides （Colsm.） Pennell.>ethanolic extract of Marchantia convluta Gao et Chang>water extract of Caryopteris incana （Thunb.）Miq.>n-butanol extract of Lindernia ruellioides （Colsm.） Pennell.>water extract of Dendrobium officinale Kimura et Migo. The change rate of rectal temperature of rabbits treated with 13 active monomers of Zhuang and Yao ethnic medicine decreased significantly at one or more time points. According to the total score of antipyretic effects, the order was as follows： Kaempferol > nobiletin > hesperidin > isoquercetin > apigenin > quercetin > astragalin > gallic acid > chlorhexidine acetate > ellagic acid > chlorogenic acid > luteolin > acacetin. Under the conditions of this experiment, the optimal dose of LPS replicating the fever model of rabbits was 4.0 μg/kg; the extracts obtained from 7 kinds of Zhuang and Yao ethnic medicine and 13 kinds of active monomers of Zhuang and Yao ethnic medicine had good antipyretic effects.

Key words: fever, endotoxin（LPS）, Zhuang and Yao ethnic medicine, active monomer, antipyretic effects

CLC Number:

R363
R285.6

DENG Ling-yu, ZHU Hua, XU Li-ba, XIE Feng-feng, LUO Jing, PING Jing-yun, NONG Li-zhen, LI Yin-lan, ZENG Yan-fang, CHEN Ya-ling, CEN Si. Establishment of LPS-induced rabbit fever model and evaluation of antipyretic effects of Zhuang and Yao medicine ingredients[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 120-129.

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