Abstract: By conducting PCR detection, genome sequencing, and evolutionary analysis on clinical waterfowl parvovirus, the genotype of the isolated strain was determined.Further selection pressure and gene recombination analysis were conducted on the genome of the isolated strains using Paml and RDP software to determine the evolutionary trend of the isolated strains. The results showed that the three strains of waterfowl parvovirus isolated belonged to the novel goose parvovirus, named YiCH strain, AnQ strain, and XiY strain, with genome lengths of 5 056, 5 056, and 5 068 bp, respectively. The three isolates had the closest phylogenetic relationship with the novel goose parvovirus M15 strain, with 6 base insertions at positions 191~196 and 4 878~4 883 in the ITR region at both ends of the XiY strain. Selection pressure analysis was conducted on the isolated strains, and three positive selection sites were detected for the VP protein of the isolated strains, namely 116Q, 261A, and 485S. Recombination analysis showed that both the YiCH and AnQ strains exhibited recombination phenomenon, with XiY strain as the main parent strain and GPV 06-0329 strain as the secondary parent strain.

Key words: waterfowl parvovirus, novel goose parvovirus, isolation and identification, select pressure, gene recombination