Research on the biological activities of terpene phenolic compounds from Plakortis simplex

doi:10.14088/j.cnki.issn0439-8114.2023.10.027

Abstract

Abstract: The chemical composition of Plakortis simplex collected from Xisha Islands in the South China Sea was studied. The terpene phenolic compounds from Plakortis simplex were tracked and enriched efficiently by LC-MS combined with HPLC. The antitumor and anti-inflammatory activities of terpene phenolic compounds were detected by MTT assay and luciferase double reporter gene assay respectively. The results showed that eight known terpene phenolic compounds, Plakordiol A（1）, Plakordiol B（2）, Plakordiol C（3）, Plakordiol D（4）, （7R,10R）-Hydroxycurcudiol（5）, （7R,10S）-Hydroxycurcudiol（6）, （7R*,10R*）-Abolene（7） and （7R*,10S*）-Abolene（8）, which were four pairs of differential isomers, were enriched and isolated from the n-butanol extract of the sponge. All the compounds showed cytotoxicity against human breast cancer MCF-7 cells at the concentration of 20 mmol/L. Plakordiol A（1） and Plakordiol C（3） showed inhibitory activity against the transcription of RXRα induced by 9-cis-retinoic acid, with significant differences compared to the model group （P<0.05 or P<0.01）. While Plakordiol D（4） promoted the transcriptional activity of RXRα, with significant differences compared with the model group （P<0.01）. Plakordiol A（1） inhibited the activation of NF-κB induced by TNF-α and had significant differences compared to the model group （P<0.05）.

Key words: Plakortis simplex, terpene phenolic compounds, antitumor activity, anti-inflammatory activity

CLC Number:

R284

LIU Xin-lian, ZHUANG Jiang-chao, ZHOU Xin-hang, TANG Ji-hang, ZHANG Sheng-yuan, WANG Jie. Research on the biological activities of terpene phenolic compounds from Plakortis simplex[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(10): 154-157.

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