Pre- and postprandial changes of orexigenic factors and anorexigenic factors in grass carp （Ctenopharyngodon idella）

doi:10.14088/j.cnki.issn0439-8114.2023.10.025

Abstract

Abstract: The transcriptional levels of ghrelin, npy, cart, cck and leptin in the brain and intestinal tissues of grass carp before and after feeding were detected by real-time fluorescence quantitative PCR to explore the variation laws of their mRNA transcripts. The results showed that transcription levels of ghrelin mRNA in the brain of grass carp reached the maximum peak 2 h before feeding （P<0.05）, and then decreased significantly, but there were no significant changes in the expression levels within 4 h after feeding （P>0.05）. The mRNA expression level of npy in the brain was significantly higher within 2 h before feeding than 2 h after feeding（P<0.05）, while its mRNA expression presented an opposite trend in the foregut. Meanwhile, the expression levels of anorexigenic factors （cck, cart and leptin） in brain and foregut kept stable at a low level 2 h before feeding, while a significant increase was observed 1~2 h after feeding in the fed group （P<0.05）. The GH content in plasma after feeding exhibited a trend of rising first and then decreasing, the peak value was delayed to 1 h after feeding.

Key words: Ctenopharyngodon idella, orexigenic factors, anorexigenic factors, GH

CLC Number:

S917.4

GUO Wei, YANG Hui-jun, MO Ai-jie, SUN Jun-xiao, ZHAI Yu-xiang, YI Lin-yuan, YUAN Yong-chao. Pre- and postprandial changes of orexigenic factors and anorexigenic factors in grass carp （Ctenopharyngodon idella）[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(10): 143-148.

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