Efficient detection method of papaya multiple PCR based on real time fluorescence quantitative PCR

doi:10.14088/j.cnki.issn0439-8114.2023.09.030

Abstract

Abstract: Based on the pCaMV35S gene nucleic acid detection kit reaction system, the target gene primers and papaya（Carica papaya L.） DNA required for multiple PCR reactions were added to conduct multiple PCR reactions. At the same time, the effect of real-time fluorescence quantification of the target gene without synthesizing fluorescent probes was investigated. The results showed that in the real-time fluorescence quantitative PCR reaction system, the gene bands amplified by multiple PCR were brighter than those amplified by conventional PCR, achieving real-time fluorescence quantitative PCR reaction of target genes without synthesizing Fluorescence probes, in order to establish a real-time fluorescence quantitative PCR detection method based on real time fluorescence quantitative PCR.

Key words: transgenic papaya （Carica papaya L.）, multiple PCR reactions, real time fluorescence quantitative PCR, fluorescence probe, PCaMV35S gene nucleic acid detection kit （PCR fluorescence probe method）

CLC Number:

S852

ZHANG Yu-shan, CHEN Jie-bin, WU Liang-jun, ZHU Xuan, OUYANG Shu-fen, SHEN Zhi-hua. Efficient detection method of papaya multiple PCR based on real time fluorescence quantitative PCR[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(9): 170-174.

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