Abstract: Ganoderma lucidum fermentation process with lotus leaf as the main substrate was studied and the content of active components before and after fermentation was determined. Biomass was selected as the detection index, and the effects of water material ratio, carbon source（glucose）, nitrogen source（soybean protein） and lotus leaf specification on Ganoderma lucidum fermentation were studied by single factor test and orthogonal test. The results showed that the optimal culture conditions of lotus leaf fermented by Ganoderma lucidum were as follows： water material ratio of 3.0∶1, carbon source（glucose） 10%, nitrogen source（soybean protein） 4%, magnesium sulfate heptahydrate 0.1 g, potassium dihydrogen phosphate 0.15 g, lotus leaf specification 40 mesh particle size, fermentation temperature 28 ℃, and fermentation time 12 d. After fermentation, the contents of active components in the substrate were alkaloid 0.508%, polysaccharide 4.870%, ganoderma acid 0.720%, soluble dietary fiber 3.450% and insoluble dietary fiber 9.020%, which was a good raw material for a functional food.

Key words: Ganoderma lucidum, fermentation process, lotus leaf, active components, orthogonal test