The effects of ampelopsin on lipopolysaccharide-induced acute lung injury mice and the TLR4/MyD88/NF-κB signaling pathway

doi:10.14088/j.cnki.issn0439-8114.2023.05.021

Abstract

Abstract: Aiming to investigate the effects of ampelopsin （APS） on lipopolysaccharide （LPS）-induced acute lung injury in mice and its underlying mechanism, the acute lung injury mouse model was established by intratracheal instillation of LPS. Seventy-two KM mice were randomly divided into 6 groups as follows： normal, model, dexamethasone and APS high-dosage, medium-dosage, and low dosage group. After 8 h of LPS induction, the animal lung function analysis system was employed to monitor pulmonary function （airway resistance, pulmonary dynamic compliance and minute ventilation volume） of mice. ELISA assay was carried out to measure the content of inflammatory factors TNF-α and IL-1β. Colorimetric assay was performed to determine MPO activity. H&E staining was performed to reveal pathological changes in lung tissues. Western blotting was performed to measure the protein expression of TLR4, MyD88, IκBα, p-IκBα, p65 and p-p65. Results showed that APS administration decreased airway resistance and increased pulmonary dynamic compliance and minute ventilation volume compared to those in the model group. Also, APS reduced TNF-α, IL-1β content and MPO activity. Further, Western blotting results demonstrated that APS down-regulated the protein expression of TLR4, MyD88, p-IκBα, p65 and p-p65, and up-regulated IκBα protein expression in LPS-induced acute lung injury. APS exerted a protective effect on LPS-induced acute lung injury in mice through regulating the TLR4/MyD88/NF-κB signaling pathway.

Key words: ampelopsin, TLR4, MyD88, NF-κB, inflammation, acute lung injury

CLC Number:

R965

ZHU Hai-bin, FANG Jing, TANG Mu-lan, CHI Xin-yu, ZENG Chun-hui, YANG Ke. The effects of ampelopsin on lipopolysaccharide-induced acute lung injury mice and the TLR4/MyD88/NF-κB signaling pathway[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(5): 118-123.

References

[1] LI W, ZHANG W, LIU J, et al. Down-regulation of miR-let-7e attenuates LPS-induced acute lung injury in mice via inhibiting pulmonary inflammation by targeting SCOS1/NF-κB pathway[J]. Biosci Rep, 2021, 41(1): BSR20201089.
[2] CHEN X, WANG H, JIA K, et al.Anti-Semaphorin-7A single chain antibody demonstrates beneficial effects on pulmonary inflammation during acute lung injury[J]. Exp Ther Med, 2018, 15(3):2356-2364.
[3] ZHANG Y, XIE Y, ZHANG L, et al.MicroRNA-155 participates in smoke-inhalation-induced acute lung injury through inhibition of SOCS-1[J]. Molecules, 2020, 25(5):1022.
[4] ZHAO J, CHEN C, GUO M, et al.MicroRNA-7 deficiency ameliorates the pathologies of acute lung injury through elevating KLF4[J]. Front immunol, 2016, 7:389.
[5] GAO J, LIU Q, LI J, et al.Fibroblast growth factor 21 dependent TLR4/MYD88/NF-κB signaling activation is involved in lipopolysaccharide-induced acute lung injury[J]. Int Immunopharmacol, 2020, 80:106219.
[6] WU Y, HUANG D, WANG X, et al.Suppression of NLRP3 inflammasome by platycodin D via the TLR4/MyD88/NF-κB pathway contributes to attenuation of lipopolysaccharide induced acute lung injury in rats[J]. Int Immunopharmacol, 2021, 96:107621.
[7] 中国科学院华南植物研究所. 中国高等植物图鉴[M].第二册.北京:科学出版社, 1990.
[8] 方静, 王元霞, 刘传梦, 等. 藤茶中双氢杨梅树皮素药理作用的研究进展[J]. 广西医学,2020, 42(12):1577-1581.
[9] 黄海涛,方静,曾春晖,等. 双氢杨梅树皮素对博来霉素诱导小鼠肺纤维化的影响[J]. 中国呼吸与危重监护杂志,2020,19(3):270-275.
[10] WANG B, XIAO Y, YANG X, et al.Protective effect of dihydromyricetin on LPS-induced acute lung injury[J]. J Leukoc Biol, 2018,103(6):1241-1249.
[11] WANG Y C, LIU Q X, ZHENG Q, et al.Dihydromyricetin alleviates sepsis-induced acute lung injury through inhibiting NLRP3 inflammasome-dependent pyroptosis in mice model[J]. Inflammation, 2019, 42(4):1301-1310.
[12] SZARKA R J, WANG N, GORDON L, et al.A murine model of pulmonary damage induced by lipopolysaccharide via intranasal instillation[J]. J Immunol Methods, 1997, 202(1):49-57.
[13] WEI X, YI X, LV H, et al.MicroRNA-377-3p released by mesenchymal stem cell exosomes ameliorates lipopolysaccharide-induced acute lung injury by targeting RPTOR to induce autophagy[J]. Cell Death Dis, 2020, 11(8):657.
[14] WANG X, DENG R, DONG J, et al.Eriodictyol ameliorates lipopolysaccharide-induced acute lung injury by suppressing the inflammatory COX-2/NLRP3/NF-κB pathway in mice[J]. J Biochem Mol Toxicol, 2020, 34(3):e22434.
[15] MCINTURFF A M, CODY M J, ELLIOTT E A, et al.Mammalian target of rapamycin regulates neutrophil extracellular trap formation via induction of hypoxia-inducible factor 1 α[J]. Blood, 2012, 120(15):3118-3125.
[16] LIU F C, YU H P, LIN C Y, et al.Use of cilomilast-loaded phosphatiosomes to suppress neutrophilic inflammation for attenuating acute lung injury: The effect of nanovesicular surface charge[J]. J Nanobiotechnology, 2018, 16(1):1-14.
[17] ÖZYURT H, ÖZDEN A S, ÇEVIK Ö, et al.Investigation into the role of the cholinergic system in radiation-induced damage in the rat liver and ileum[J]. J Radiat Res, 2014, 55(5):866-875.
[18] PITTET JF, KOH H, FANG X, et al.HMGB1 accelerates alveolar epithelial repair via an IL-1β- and αvβ6 integrin-dependent activation of TGF-β1[J]. PLoS one, 2013, 8(5):e63907.
[19] MERTOWSKI S, GRYWALSKA E, GOSIK K, et al.TLR2 expression on select lymphocyte subsets as a new marker in glomerulonephritis[J]. J Clin Med, 2020, 9(2):541.
[20] BUTT Y, KURDOWSKA A, ALLEN T C.Acute lung injury: A clinical and molecular review[J]. Arch Pathol Lab Med, 2016, 140(4):345-350.