Antifungal activity of chemical constituents from Mikania micrantha against 6 species of Juglans regia pathogenic fungi

doi:10.14088/j.cnki.issn0439-8114.2023.05.012

Abstract

Abstract: In order to explore the antifungal effects of the chemical constituents of invasive Mikania micrantha, five compounds were isolated from the ethyl acetate extract of the aerial part of Mikania micrantha by chromatographic methods such as silica gel column chromatography and Sephadex LH-20 gel column chromatography. According to their ¹H NMR, ¹³C NMR and MS spectral data, the five isolated compounds were identified as Stigmasterol（1）, Mikanolide（2）, Deoxymikanolide（3）, 3,5,4′-trihydroxy-6,7-dimethoxy-flavone（4） and Nepetin（5）, respectively. The antifungal activities of different solvent extracts and isolated compounds from Mikania micrantha against 6 species of pathogenic fungi of walnut were determined by the petri plate method. The results showed that the ethyl acetate extract and the n-butanol extract had a high inhibitory effect on the mycelia growth of Colletotrichum sp. and Phyllosticta sp., with EC₅₀ of 1.250 00 mg/mL and 1.660 00 mg/mL, respectively. Compounds 2 and 3 showed higher inhibition rats against Phompsis sp. and Fusicoccum sp. than other compounds. The EC₅₀ of compounds 2 and 3 against Phompsis sp. were 60.62 μg/mL and 89.13 μg/mL, and against Fusicoccum sp. were 43.65 μg/mL and 61.66 μg/mL, respectively.

Key words: invasive plant, Mikania micrantha, chemical constituents, Juglans regia, antifungal activity

CLC Number:

S482.2

ZHANG Wei, QI Jin-kang, LI Jin-fang, HU Shi-jun, YAN Xiao-hui. Antifungal activity of chemical constituents from Mikania micrantha against 6 species of Juglans regia pathogenic fungi[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(5): 66-72.

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