Full-length transcriptome sequencing and analysis of yellow catfish based on PacBio platform

doi:10.14088/j.cnki.issn0439-8114.2023.04.035

Abstract

Abstract: In order to further enrich the genomic information database of yellow catfish （Pelteobagrus fulvidraco）, the full-length transcriptome of liver, kidney, muscle, brain, spleen, heart, skin, blood, gill, and gonad tissues of yellow catfish was sequenced using the PacBio sequel platform. A total of 685 574 full-length non-chimeric read （FLNC） sequences were obtained, and 72 509 non-redundant isoforms with an average length of 2 918 bp were acquired by aligning with the genome of yellow catfish. 3 169 LncRNA, 45 872 alternative splicing events, 4 881 genes with variable polyadenylation sites, and 304 fusion genes were identified. After analyzing the 12 492 identified new genes in five public databases including NR, GO, KEGG, KOG, and Swissprot, 7 233 isoforms were successfully annotated. The new genes were mainly enriched in the Cellular process, Cell, and Binding categories according to GO analysis. KEGG pathway analysis indicated that the new genes were mainly enriched in the Signal transduction and Immune system signaling pathways. In addition, the metabolic pathways of the endocrine system related to reproduction and breeding of yellow catfish included the Oxytocin, Estrogen, Progesterone-mediated oocyte maturation, Gonadotropin releasing hormone, and Ovarian steroidogenesis signaling pathways.

Key words: yellow catfish（Pelteobagrus fulvidraco）, PacBio, full-length transcriptome, alternative splicing, signaling pathway

CLC Number:

S917

WANG Jia-qi, XIONG Yang, HAN Qing-qing, HUANG Pei-pei, MEI Jie. Full-length transcriptome sequencing and analysis of yellow catfish based on PacBio platform[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(4): 194-201.

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