Molecular identification of Bactrocera minax larvae in Taihu Lake County based on DNA barcode technology

doi:10.14088/j.cnki.issn0439-8114.2023.04.034

Abstract

Abstract: In order to accurately identify the fruit fly larvae collected from Tianhua Town, Taihu Lake County, Anhui Province, five fruit fly larvae were randomly selected as sample materials, and their molecular identification was carried out using DNA barcode technology and PCR technology. The results showed that the DNA bar code sequence of the fruit fly larva sample was 100% similar to the standard sequence of Bactrocera minax in BOLD database; the phylogenetic tree was constructed using the MAGA-X software. The sample sequence of fruit fly larvae and the Bactrocera minax sequence in the NCBI database formed a single line, which was clearly distinguished from its related species; PCR was carried out with specific primers of citrus fruit fly, and the length of the product was consistent with the characteristics of the amplified sequence length of the specific primers, indicating that the five samples of fruit fly larvae were Bactrocera minax.

Key words: Bactrocera minax, DNA barcode, PCR, COI gene, molecular identification, Anhui Province

CLC Number:

JIA Xiao-yun, XING Yao-chun, MA Xiao-jing, LI Kai-qiu, ZHUO Zhi-bin, WU Jun-xia, YANG Wen-zhao, ZENG Ling-yu, ZHAO Yan, WANG Yu-ning, LI Zhi-hong. Molecular identification of Bactrocera minax larvae in Taihu Lake County based on DNA barcode technology[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(4): 190-193.

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