Abstract: To understand the infrared spectrum characteristics of the body hair of different parts of Myodes rufocanus, Fourier transform infrared spectroscopy （FT-IR） and second derivative infrared spectroscopy （SD-IR） were used to analyze the dorsal and abdominal hairs of the Myodes rufocanus. Results showed that the infrared spectrum of dorsal and abdominal hairs formed obvious absorption peaks around 1 560 cm^-1（amide Ⅱ band） and 1 637 cm^-1（amide Ⅰ band）. The bending vibration absorption peak of O=C-N groups of the amide Ⅳ band, one of the typical amide group absorption characteristic peaks in the protein infrared spectrum, appeared in the same position （621, 643 cm^-1）. The bending vibration absorption peaks of N-H groups in the amide Ⅴ band all appeared in 678, 781 cm^-1, but at the same time, the back hair had the bending vibration absorption peaks of N-H groups in the amide side band in 685 cm^-1, but the abdominal hair was not observed. In the second derivative spectra of the back hair and abdominal hair, the absorption peaks in 702, 777, 786, 1 646, 1 633 and 1 654 cm^-1 were basically the same. However, the back hair had a smaller peak in 726 cm^-1, while the abdominal hair had a distinct single peak in 1 687~1 694 cm^-1, and the back hair had an indistinct W-shaped pattern in this position. The back hair and abdominal hair of Myodes rufocanus could reflect the characteristics of the species. In the range of 600~800 cm^-1, the amide group was more obvious, and the infrared spectrum and second derivative spectrum of back and abdominal hair were very similar. In addition, there were differences in the infrared spectrum of Myodes rufocanus back hair and abdominal hair, especially in the range of 600~800 cm^-1 and 1 600~1 800 cm^-1.

Key words: Myodes rufocanus, body hair, Fourier transform infrared spectroscopy （FT-IR）, Second derivative infrared spectroscopy （SD-IR）