Acquisition and detection of co-transformed 6-SFT and bar expression frames in wheat

doi:10.14088/j.cnki.issn0439-8114.2022.24.038

Abstract

Abstract: To investigate the feasibility of fructan synthase gene 6-SFT derived from Psathyrostachys huashanica and Haynaldia villosa to improve the resistance of wheat （Triticum aestivum L.） to abiotic stress, the Ph-6-SFT or Dv-6-SFT gene was introduced into the young embryonic healing tissue of the common wheat family Kenong 199 by gene gun-mediated method using bar as a screening marker gene, and resistant plants were obtained by using the herbicide glufosinate as a screening agent and identified by PCR and GUS histochemical staining. The results showed that 146 and 187 resistant plants were obtained by the PPT screening system, of which 4 and 6 were PCR positive and 1 was GUS histochemically stained, respectively, and the transformation rates were 0.24% and 0.19%, respectively. The results showed that Ph-6-SFT and Dv-6-SFT genes had been integrated into the wheat genome, respectively, and transgenic wheat without antibiotic marker genes was obtained.

Key words: Triticum aestivum L., co-transformation, Ph-6-SFT gene, Dv-6-SFT gene, bar gene, expression cassettes

CLC Number:

S188

HE Xiao-lan, WANG Jian-wei, CHEN Xin-hong, LI Wen-xu, QIN Shao-zhao, TANG Hong, WU Xian-zhi. Acquisition and detection of co-transformed 6-SFT and bar expression frames in wheat[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(24): 185-188.

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