Bioinformatics prediction of Microdera punctipennis Attacin genes structure and function

doi:10.14088/j.cnki.issn0439-8114.2022.24.036

Abstract

Abstract: This study aimed to understand the structure and function of the antimicrobial peptide genes MpAtt1 and MpAtt2 of Microdera punctipennis. Physicochemical properties of amino acids, the signal peptide, transmembrane structure, protein secondary structure, protein posttranslational modification, functional structural domain, protein tertiary structure and subcellular localization, homology analysis of amino acid encoded by the two Attacin genes were conducted bioinformatics analysis with the help of some bioinformatics software. The secondary structures of MpAtt1 and MpAtt2 were dominated by random coil and β-sheet, with a large number of potential phosphorylation sites, and the tertiary structures were highly conservative with Attacin_C domain and Gloverin domain, belonging to the Attacin_C superfamily. MpAtt1 and MpAtt2 proteins were soluble secretory proteins, containing N-terminal signal peptide.The two Attacin genes showed 84.77% identity with Attacin1 gene of Anatolica polita at the amino acid level. It was shown that MpAtt1 and MpAtt2 had good antibacterial activity, providing a theoretical basis for the functional identification of this gene, with a view to providing a gene source for the study of the cold tolerance mechanism of Microdera punctipennis.

Key words: Microdera punctipennis, antimicrobial peptide, Attacin, structure, bioinformatics

CLC Number:

Q966
S433.5

LI Jie-qiong, LI Xiao-wan. Bioinformatics prediction of Microdera punctipennis Attacin genes structure and function[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(24): 170-176.

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