Abstract: Based on the previous mulberry transcriptome data and photosynthetic phenotype data,through WGCNA analysis, 10 gene modules associated with the phenotype were obtained, and screenedthe photosynthetic-related gene modules of MEmagenta, MEgreen, MEyellow, MEpurple, and MEblack were screened. GO and KEGG analysis showed that DEGs were significantly enriched in the activities of photosynthesis-related enzymes, carbon metabolism and carbon fixation. 12 genes highly related to photosynthesis were screened. Finally, through RNA-seq combined with WGCNA analysis, three key photosynthetic genes were selected, namely, photosystem Q（B） protein L484_000029 gene, ribose bisphosphate carboxylase L484_000836 gene, and ribose bisphosphate carboxylase/oxygenase activator 2 L484_025354 gene. The results provided support for further research on the regulation mechanism of mulberry photosynthesis.

Key words: WGCNA, mulberry, photosynthesis-related genes