Analysis of immune-related genes based on the transcriptome of Microdera punctipennis

doi:10.14088/j.cnki.issn0439-8114.2022.22.033

Abstract

Abstract: The transcriptome data of the beetle Microdera punctipennis was used to annotate and screen its immune-related genes. Microdera punctipennis transcriptome library contained 198 206 unigenes. A total of 49 034 genes were annotated, accounting for 24.74% of the total transcriptome unigenes. 1 750 homologous unigene of 107 immune-related genes in Microdera punctipennis, which mainly encoded pattern recognition receptors, immune signal transduction and regulatory factors and immune effectors, were obtained by annotation information and manual screening. The results showed that the Toll, MAPK-JNK-p38 and JAK-STAT immune pathways were intact and conserved in Microdera punctipennis, and most of their immune-related cellular processes, such as autophagy, apoptosis and RNA interference and their mediators and modulators, were also conserved, along with a variety of immune effectors, including antimicrobial peptides, heat shock proteins, and enzymes such as lysozyme, phenoloxidase, chitinase and antioxidase. However, conserved immune-related genes such as IMD, FADD and Dome, which were commonly found in other insects, were not identified in Microdera punctipennis. A preliminary map of the immune pathway in Microdera punctipennis was made based on the screened immune-related genes. The established framework of the immune system of the beetle might provide a reference for the study of immune-related genes in non-Drosophila insects.

Key words: immune related gene, Microdera punctipennis, transcriptome, immune signaling pathway, immune effector

CLC Number:

Q966
S433.5

LI Jie-qiong. Analysis of immune-related genes based on the transcriptome of Microdera punctipennis[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(22): 181-194.

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