Abstract: In order to establish the seed batch of Clostridium perfringens type C,taking the second generation basic seeds of C. perfringens C59-2 strain as the original seeds, it was continuously subcultured to 13 generations by inoculating the appropriate medium. The basic seeds of generation 1（F₁）, generation 5（F₅）, generation 10（F₁₀） and generation 13（F₁₃） were carried out purity test, culture characteristics, biochemical characteristics, toxin production ability, serotype and immunogenicity identification. The test results of immunogenicity identification showed that all indexes were qualified. It could be used for the production of piglet colibacillosis and Clostridium perfringens type C inactivated vaccine. The basic seed batch was F₁~F₁₀ generation.

Key words: Clostridium perfringens type C, seed batch, establishment, identification