Study on physiological and biochemical characteristics of Cercidiphyllum japonicum during flower bud development

doi:10.14088/j.cnki.issn0439-8114.2022.22.017

Abstract

Abstract: By detecting the changes of isozyme activity, endogenous soluble substance and malondialdehyde content in flower buds of Cercidiphyllum japonica at different developmental stages and different sexes, the development regularity of flower buds and the adaptation strategy to the environment were explored at the physiological and biochemical level. The activities of peroxidase and superoxide dismutase, the contents of soluble protein, soluble sugar and malondialdehyde in flower buds of Cercidiphyllum japonica were determined by guaiacol method, nitrogen blue tetrazole method, Coomassie Bright Blue G-250 method, anthrone method and 2-thiobarbituric acid （TBA） method, respectively. The results showed that during flower bud development, the activity of peroxidase increased at first and then decreased, the activity of superoxide dismutase fluctuated at a certain level, and the contents of soluble protein, soluble sugar and malondialdehyde increased at first and then decreased; the superoxide dismutase activity, soluble protein and malondialdehyde contents in female flower buds were higher than those in male flower buds.

Key words: Cercidiphyllum japonica, flower bud development, physiological and biochemical indexes, environmental adaptation

CLC Number:

Q945

DUAN Qiu-di, YANG Xu, ZHAO Yan, LU Li-jing, CUI Xiu-wei, LIANG Hong-wei. Study on physiological and biochemical characteristics of Cercidiphyllum japonicum during flower bud development[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(22): 89-93.

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