RAPD analysis of intraspecial and interspecific genetic diversity in Rubus chingii Hu

doi:10.14088/j.cnki.issn0439-8114.2022.21.034

Abstract

Abstract: Rubus chingii Hu, belongs to Rubus, Rosaceae. Its unripe fruit is used as medicine material, and its ripe fruit is rich in nutritions. Rubus chingii is a unique dual-function food with remarkable nutraceutical, pharmaceutical and economic values. It is urgent to develop molecular marker-assisted breeding and then comprehensively improve the quality of Rubus chingii fruit. Seventeen accessions of screened Rubus chingii seedlings with good traits and seven other Rubus plants, including Rubus corchorifolius,Rubus trianthus,Rubus parvifolius,Rubus lambertianus, Rubus tsangii, as well as Rubus fructicosus var. Hull and Rubus rosifolius var. coronarius, were used as materials, to select the optimal RAPD primers for intraspecial and interspecific genetic diversity analysis for Rubus chingii. The results showed 25 primers with products of clear bands and good repeatability could be used to identify the Rubus chingii seedlings and other Rubus plants. Furthermore, six primers could be applied for distinguishing different genotypes of Rubus chingii.

Key words: Rubus chingii Hu, Rubus, molecular marker, random amplified polymorphic DNA

CLC Number:

CHEN Yi-quan, CHEN Zhen, JIANG Jing-yong, LIU Hao-zheng, TANG Yu-jie. RAPD analysis of intraspecial and interspecific genetic diversity in Rubus chingii Hu[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(21): 183-188.

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