Abstract: In June 2021, a Longlin goat farm in Guangxi suddenly experienced a decrease in the appetite of the sheep, and some goats were lying on the ground, coughing, wheezing, purulent nasal discharge, elevated body temperature, diarrhea and other symptoms, and even death, and the sick and dead sheep were dissected and conducted isolation and identification of bacteria. The results showed that the isolated strains were mainly based on sepsis, hemorrhagic enteritis and fibrinous pneumonia, and the isolated strains were morphologically characterized and 16S rDNA sequenced. The case was therefore sepsis caused by a mixture of infections with E.coli, Pasteurella multiclimum D, Acinetobacter india, Aeromonas guinea pig, and Staphylococcus coronaria. Susceptibility test results showed that bacteria were highly sensitive to kanamycin, cefotaxime, telmicoxam, and florfenicol.

Key words: bacterial isolation and identification, susceptibility test, 16S rDNA sequencing