HUBEI AGRICULTURAL SCIENCES ›› 2022, Vol. 61 ›› Issue (19): 160-163.doi: 10.14088/j.cnki.issn0439-8114.2022.19.030

• Biological Engineering • Previous Articles     Next Articles

Study on tissue culture seedling technology of Trichosanthes kirilowii Maxim.

LI Fen, ZENG Wen-hao, ZHU Jian-feng   

  1. Hubei Ecology Polytechnic College,Wuhan 430200,China
  • Received:2022-04-18 Online:2022-10-10 Published:2022-11-04

Abstract: The tissue culture system of Trichosanthes kirilowii Maxim.was successfully established by using the young shoots as explants. The results showed that the most suitable disinfection method for explants was to use 75% ethanol for 20 s and then 0.1% HgCl2 for 4 min. The best induction medium was MS+2.0 mg/L KT+0.4 mg/L IAA, the best proliferation medium was MS+3.0 mg/L KT+0.4 mg/L IAA, and the best rooting medium was 1/4 MS+0.2 mg/L NAA. The best planting substrate volume ratio for transplanting was perlite ∶ peat ∶ vermiculite = 1∶2∶1.

Key words: Trichosanthes kirilowii Maxim., tissue culture, seedling raising

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