Optimization of enzyme-assisted aqueous extraction technology of sapindus saponins

doi:10.14088/j.cnki.issn0439-8114.2022.16.032

Abstract

Abstract: The sapindus saponins was extracted by enzyme extraction, the effects of different cellulase on the extraction rate of saponins from sapindus with different particle size were investigated,and the extraction conditions of sapindus with large particle size including the dose of cellulase,extraction time,extraction temperature were optimized. The results showed that cellulase had a little or no effect on the saponins extraction rate of sapindus powder greater than sieve 5, and those larger than sieve 5 were not extracted with cellulose. The best process condition for sieve 1-2 particle powder was enzyme addition of 18 U/mL, extraction time of 2.5 h, and extraction temperature of 50 ℃. The best process condition for sieve 2-3 particle powder was enzyme addition of 12 U/mL, extraction time of 1.5 h, and extraction temperature of 50 ℃. This method was environment-friendly and profitable,which could provide reference for industrial extraction of sapindus saponins and the classification of raw material granularity.

Key words: cellulase, sapindus saponins, granularity, extraction rate

CLC Number:

R284.2

PENG Yi-xin, FU Chun-hua, WANG Ya, WU Ze-jun. Optimization of enzyme-assisted aqueous extraction technology of sapindus saponins[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(16): 164-168.

References

[1] 伍恒, 张翠, 翁震,等. 无患子总皂苷质控品的制备[J]. 食品工业科技, 2013, 34(8):91-96.
[2] 黄素梅,王敬文,杜孟浩,等.无患子总皂苷的提取工艺研究[J].安徽农业科学, 2010, 38(1):354-356.
[3] 李芳,霍光华,叶亚建,等.无患子皂苷的提取、类型及药理活性[J].天然产物研究与开发,2012,24(S1): 158-164,181.
[4] 伍恒, 王娜, 翁震,等. 无患子皂苷及复配体系表面活性的研究[J]. 精细化工, 2013,30(2):149-154.
[5] SPARG S G, LIGHT M E, STADEN J V.Biological activities and distribution of plant saponins[J]. Journal of ethnopharmacology,2004,94(2-3):219-243.
[6] 阳春苗, 王秋玲, 梁芳,等. 响应面法优化无患子皂苷超声提取工艺研究[J]. 生物化工,2021,7(1):1-4,10.
[7] 谭明辉, 霍光华. 无患子总皂苷的提取工艺及其抑菌活性研究[J]. 湖北农业科学, 2014,53(22):5511-5514.
[8] 但汉龙, 李娜, 叶红莲,等. 响应面优化无患子皂苷超声辅助提取工艺[J]. 中南林业科技大学学报, 2015,35(6):107-113.
[9] 付盼娟. 无患子皂苷的分离纯化及性能研究[D]. 上海:上海应用技术大学, 2016.
[10] 马丽珍. 无患子皂苷提取工艺的优化[J].日用化学工业,2015,45(3):149-151.
[11] 冯月燕, 李智斌. 天然无患子总皂苷提取工艺研究[J]. 广东化工,2019,46(9):128-129.
[12] 黄浩庭, 欧霖拱, 于玲,等. 无患子皂苷的分析方法和提取工艺优化研究[J]. 日用化学品科学, 2015, 38(12):42-44.
[13] 郑秀玉, 吴海华, 陈赟. 微波辅助法提取无患子皂苷的工艺优化[J]. 湖北农业科学, 2013,52(20):5026-5028.
[14] 余美琼, 朱金环, 杨金杯,等. 复合酶法辅助提取无患子总皂苷的工艺[J]. 福建师大福清分校学报, 2020(2):44-49.
[15] 张永忠, 徐建军, 叶玉杰. 无患子果皮皂苷提取及天然洗手液制备研究[J]. 亚太传统医药, 2017, 13(14):34-36.
[16] 马晓江, 王进,汤锋,等. 无患子总皂苷减压提取工艺优化及其除草活性研究[J]. 林产化学与工业,2019,39(1):108-114.
[17] 刘俊宏, 邱智东. 无患子总皂苷提取工艺优化及其对酪氨酸酶的抑制活性[J]. 江苏农业科学, 2020, 48(9):228-232.
[18] 杨力,贾桂云.酶解法提取海南龙血树叶总皂苷的工艺研究[J].海南师范大学学报(自然科学版),2012,25(4):421-424.
[19] 雷蕾, 张炜, 刘龙,等. 复合酶协同超声提取藜麦皂苷及其抗氧化性[J]. 精细化工, 2019, 36(3):469-474,493.
[20] 魏凤玉,方春.酶法提取无患子皂苷的工艺研究[J].应用化工,2010,39(8):1149-1151.
[21] 郑林禄,朱梅花,陈峰,等.复合酶法提取无患子总皂苷的工艺研究[J].高师理科学刊, 2017,37(6):55-57,60.