HUBEI AGRICULTURAL SCIENCES ›› 2022, Vol. 61 ›› Issue (15): 218-221.doi: 10.14088/j.cnki.issn0439-8114.2022.15.041

• Biological Engineering • Previous Articles     Next Articles

Research on tissue culture and rapid propagation technology of European rose ruby ice

LI Fen, LIU Mu-qing, ZHU Jian-feng   

  1. Hubei Ecology Vocational College,Wuhan 430200,China
  • Received:2022-03-25 Online:2022-08-10 Published:2022-08-25

Abstract: The semi-lignified middle stem segments of European rose (Rosa chinensis Jacq.)variety ruby ice were used as explants, and the tissue culture system was successfully established. The results showed that the best sterilization method for explants was 0.1% HgCl2 for 8 min; the optimal medium formula in the primary culture stage was MS+0.2 mg/L NAA+2.0 mg/L 6-BA, the optimal medium formula in the subculture proliferation stage was MS+0.2 mg/L NAA+2.5 mg/L 6-BA, and the optimal medium formula in the rooting culture stage was 1/2 MS+0.2 mg/L NAA. The best transplanting substrate was perlite+peat+vermiculite, and the volume ratio was 1∶1∶1.

Key words: European rose(Rosa chinensis Jacq.), tissue culture, rapid reproduction

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