HUBEI AGRICULTURAL SCIENCES ›› 2022, Vol. 61 ›› Issue (15): 214-217.doi: 10.14088/j.cnki.issn0439-8114.2022.15.040

• Biological Engineering • Previous Articles     Next Articles

Study on tissue culture system of Catalpa fargesii Bureau

YANG Yu-zhen1, LI Xiao-zhe2, HOU Hong-wei2   

  1. 1. Center of Plant Tissue Culture, Nanyang Vocational College of Agriculture,Nanyang 473000, Henan,China;
    2. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  • Received:2021-10-09 Online:2022-08-10 Published:2022-08-25

Abstract: Catalpa bungei C.A. Mey. is a unique native tree species and famous ornamental tree species in China. The optimal stem segments of native species of Catalpa fargesii Bureau in Nanyang, Henan were used as explants to study the induction of cluster buds and rooting of strong seedlings. The results showed that the optimal ratio of growth regulators for the induction and proliferation of clustered buds was MS+1.0 mg/L 6-BA+0.5 mg/L GA3+0.5 mg/L IBA. The optimal ratio of growth regulators for the cultivation of strong seedlings was MS+0.6 mg/L 6-BA+1.0 mg/L IBA. The optimal rooting rate of seedlings was 1/2 MS+0.5 mg/L IBA.

Key words: Catalpa fargesii Bureau, tissue culture, rapid propagation

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