Abstract: HydroBondPS-C₁₈ Column（4.6 mm×250.0 mm,5 μm） was used for gradient elution with acetonitrile and 0.2% formic acid water as the mobile phase. The flow rate was 1.0 mL/min, the column temperature was 30 ℃, and the detection wavelengths were 203 and 242 nm. The results showed that the linear ranges of pachymaric acid A, pachymaric acid and pineosinic acid were 1.82~10.94 μg, 1.90~11.38 μg, 2.21~13.26 μg, respectively. The correlation coefficient was 0.999 9, indicating that there was a good correlation between the two variables. The recovery rates were 99.32%, 99.85% and 99.01%, respectively, and the fingerprint of Poria cocos was established. There were 11 common peaks of Poria cocos in 11 regions. Among them, the contents of pachymaric acid A, pachymaric acid and pineosinic acid in Xichang City of Sichuan Province were the highest, which were 4.21,4.39 and 4.06 mg/g, respectively. This method has good stability and high repeatability, which is suitable for the establishment of Poria cocos fingerprint and the determination of triterpenoid acid content.

Key words: Poria cocos, fingerprint, triterpene acid, content determination