Abstract: HPLC method was established to simultaneously determine the contents of puerarin, dihydromyricetin and myricetin in Wuwei Quzhi granules. Shim-pack C₁₈ column was used, the mobile phase was acetonitrile-0.1% phosphoric acid, and gradient elution was adopted; the flow rate was 1.0 mL/min, the column temperature was 30 ℃, and the detection wavelengths were 250 and 285 nm. The results showed that puerarin, dihydromyricetin and myricetin had a good linear relationship at the levels of 0.023 0~0.138 0 μg, 1.087 2~6.523 2 μg and 0.500 8~3.004 8 μg, respectively. The average recoveries of puerarin, dihydromyricetin and myricetin were 102.7%, 101.1% and 99.7%, respectively, with RSDs of 2.56%, 2.21% and 0.78%, respectively. The method is stable, accurate and reproducible, and can be used for quality control of Wuwei Quzhi granules.

Key words: Wuwei Quzhi granules, HPLC, puerarin, dihydromyricetin, myricetin