Isolation and identification of endophytic fungi from Amepelopsis grossedentata and its effects on selenium enrichment in host

doi:10.14088/j.cnki.issn0439-8114.2022.15.019

HUBEI AGRICULTURAL SCIENCES ›› 2022, Vol. 61 ›› Issue (15): 117-120.doi: 10.14088/j.cnki.issn0439-8114.2022.15.019

• Resource & Environment • Previous Articles Next Articles

Isolation and identification of endophytic fungi from Amepelopsis grossedentata and its effects on selenium enrichment in host

ZHOU Fang-zhen^1,2, ZHOU Zhi^1,2, LIU Shui-song², GONG Qi-wen², CHEN Meng-jie², YAN Peng-fei²

1. Hubei Engineering Research Center of Selenium Food Nutrition and Health Intelligent Technology, Enshi 445000, Hubei, China;
2. College of Biological Science and Technology, Hubei Minzu University, Enshi 445000, China

Received:2021-09-02 Online:2022-08-10 Published:2022-08-25

Abstract

Abstract: To investigate the effect of endophytic fungi on selenium accumulation in vine tea （Amepelopsis grossedentata）, the dominant endophytic fungi were isolated from vine tea seed and identified by morphological observation and rDNA-ITS sequence analysis. Sodium selenite solution was injected into the rhizosphere of vine tea seedlings, and high and low concentrations of endophytic fungal spore suspension were prepared and applied to the leaf surface of vine tea seedlings. Vine tea samples were collected in time. After drying, the selenium content was determined by atomic fluorescence spectrometry. The results showed that the dominant endophytic fungus LZC-1 was isolated and identified as Fusarium sp.; LZC-1 treatment could significantly reduce the enrichment of selenium in soil by vine tea （P<0.01）, and the inhibitory effect of high concentration spore suspension on host selenium enrichment was significantly stronger than that of low concentration spore suspension （P<0.05）. From this it can be seen that selenium application can increase the selenium enrichment of vine tea, while endophytic fungus LZC-1 can reduce the selenium enrichment of host vine tea.

Key words: vine tea（Amepelopsis grossedentata）, endophytic fungi, selenium enrichment

CLC Number:

Q935

ZHOU Fang-zhen, ZHOU Zhi, LIU Shui-song, GONG Qi-wen, CHEN Meng-jie, YAN Peng-fei. Isolation and identification of endophytic fungi from Amepelopsis grossedentata and its effects on selenium enrichment in host[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(15): 117-120.

References

[1] 安利峰, 胜利, 范桂香. 硒的功能及其相关疾病[J].国外医学:医学地理分册,2005,26(2):56-57,79.
[2] 杜振宇,史衍玺,王清华. 蔬菜对硒的吸收及适宜补硒食用量[J]. 生态环境, 2004,13(2):230-231,267.
[3] 周防震, 郑小江, 张晓元. 藤茶活性成分二氢杨梅素对人胚肾细胞和干细胞增殖的影响[J]. 中成药, 2015,37(12):2756-2758.
[4] 周防震,郑小江. 中国良种——藤茶[M]. 湖北武汉:湖北科学技术出版社,2016.
[5] 周成河, 许敏, 顿春垚, 等. 硒肥浓度对藤茶硒含量及生长发育的影响[J].食品工业科技,2017,38(2):127-131.
[6] 张朝阳, 黄光昱, 陈永波, 等. 根施硒肥对藤茶硒含量及产量的影响[J].湖北民族学院学报(自然科学版),2019,37(2):131-133,185.
[7] GB5009.93—2017, 食品中硒的测定[S].
[8] ZHOU F Z, ZHANG X Y, ZHAN Y J, et al.Dihydromyricetin inhibits cell invasion and down-regulates MMP-2/-9 protein expression levels in human breast cancer cells[J]. Progress in biochemistry and biophysics,2012,39(4):352-358.
[9] ZHENG X J, XIAO H, ZENG Z, et al.Composition and serum antioxidation of the main flavonoids from fermented vine tea(Ampelopsis grossedentata)[J]. Journal of functional foods,2014(9):290-294.
[10] 唐德英,李学兰,张丽霞,等.一种促进藤茶种子发芽的方法[P].中国专利:CN110313266A,2019-10-11.
[11] 张友胜, 宋松泉, 刘军. 藤茶的人工栽培[J]. 生命世界, 2021(5):34-35.
[12] 牛铁荃,郭雯岩,李雪雪,等. 葡萄砧木实生苗的抗寒性评价[J]. 山西农业大学学报(自然科学版),2020,40(6):29-37.
[13] 唐文华, 苟体忠, 蒋天智, 等. 柿树不同部位中硒的分布特征研究[J]. 广东化工,2015,42(22):41.
[14] 黄姣, 郑智溢, 蔡林邑. 茶树不同部位硒含量分析及其土壤有效态硒的关系研究[J]. 中国卫生检验杂志,2015,25(18):3169-3170.
[15] 雷红灵,周大寨, 郑小江, 等. 叶面喷硒对藤茶硒含量及品质的影响[J]. 西北农林科技大学学报(自然科学版),2012, 40(2):190-194.

Isolation and identification of endophytic fungi from Amepelopsis grossedentata and its effects on selenium enrichment in host

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	QUE Xiao-feng, YU Yan, FANG Zhi-cheng, SI Wen-hui, HU Xue-ping. Research of selenium enrichment characteristic by foliar fertilization and soluble speciation analysis in grape [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(7): 31-34.
[2]	GOU Wen-bo, YANG Zhong-duo, ZHOU Ge-ge. Isolation and anti-monoamine oxidase activity of endophytic fungi from four medicinal plants [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 56-59.
[3]	FAN Hai-yun, TENG Meng-ting, ZHOU Sheng-liang, MA Lin, LI Ying, LIU Xin-xin, DING Ru-ru, ZHANG Peng. Identification and study of eight endophytic fungi with antimicrobial activity from Lobaria sp. [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(12): 92-96.
[4]	WEN Chun-nan, WANG Xiao-long, LI Shou-ting, ZHANG Peng-zhan, YE Ying-xiao, MA Bing-ji. Isolation and Identification of Endophytic Fungi from Dioscorea opposita Thunb [J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(8): 58-60.