Abstract: In order to further understand the structure and function of fish-related genes and lay the foundation for the follow-up study of PI3K/AKT signaling pathway and key genes, we studied the key genes family of black seabream antifreeze. Based on the existing gene family members, AKT and CK gene family members were screened from the genome of Sparus macrocephalus. Their molecular weight, isoelectric point, motif structure and conserved domain were analyzed, and the phylogenetic tree was constructed with related species. The results showed that the AKT gene family proteins of fish were highly conserved, and the AKT sequence was clustered into a single branch. Among them, AKT1 and AKT3 were closely related to those of glithead seabream, and they were clustered into a branch with Pseudosciaena crocea. AKT2 is clustered with fish, but had a distant genetic relationship with other fish. Muscle creatine kinase （mck） and brain creatine kinase （bck） were identified in black seabream. The length and molecular weight of mck and bck were basically the same, and both of them contained creatine kinase domain. The mck and bck of mammals were closely related to the bck of fish. However, compared with fish, it formed a branch. In mck, black seabream has a close genetic relationship with glithead seabream, while in bck, black seabream has the closest genetic relationship with large yellow croaker and separates into another branch compared with other fishes.

Key words: low temperature stress, signal pathway, hypothermic sensor, gene family, AKT gene, CK gene