Identification of Euphorbia maculata L. and its adulterants in Guangxi based on DNA barcode technology

doi:10.14088/j.cnki.issn0439-8114.2022.10.028

Abstract

Abstract: In order to identify the species of Euphorbia maculata L. and its adulterants,the total genomic DNAs were extracted from 41 samples of Euphorbia maculata L. and its adulterants from Guangxi, and the barcode rbcL and psbA-trnH sequences of each sample were amplified and sequenced, and compared with the rbcL and psbA-trnH sequences of 13 samples downloaded by GenBank. The intraspecific and interspecific genetic distances of Euphorbia maculata L. and its adulterants were calculated and the phylogenetic tree was constructed by the neighbor-joining method. The results showed that the intraspecific genetic distance was significantly smaller than the interspecific genetic distance between Euphorbia maculata L. and its adulterants. The phylogenetic tree analysis results showed that the samples of Euphorbia maculata L. were clustered into a single line,which indicated that rbcL and psbA-trnH sequences could distinguish Euphorbia maculata L. and its adulterants.

Key words: Euphorbia maculata L., adulterants, DNA barcode, genetic distance, phylogenetic tree

CLC Number:

S567.219

HOU Jing, HUANG Yong, LI Li, WANG Su-e, CAI Yi. Identification of Euphorbia maculata L. and its adulterants in Guangxi based on DNA barcode technology[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(10): 156-159.

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