Analysis on the ploidy of hybrid seeds between Lagerstroemia indica and  Lagerstroemia speciosa

doi:10.14088/j.cnki.issn0439-8114.2022.10.017

Abstract

Abstract: Using Lagerstroemia caudata as comparison, L.fauriei, L.limii,L. speciosa, L. indica ‘Dynamite’ and 12 interspecific hybrids between L. indica and L. speciosa were diploid by detecting with flow cytometry. Eight SSR markers were used to determine the ploidy of Dynamite, L. speciosa, L. limii and 26 interspecific hybrids between L. indica and L. speciosa, and these plants were diploid. These results confirmed the feasibility of flow cytometry and SSR markers to reflect plant ploidy.

Key words: Lagerstroemia indica, Lagerstroemia speciosa, interspecific hybridization, flow cytometry, SSR, ploidy

CLC Number:

S685.99

LI Zhen-fang, PENG Chan, HUANG Guo-wei, MA Lin-jiang, KE Zun-fa, YANG Yan-ling. Analysis on the ploidy of hybrid seeds between Lagerstroemia indica and Lagerstroemia speciosa[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(10): 99-105.

References

[1] WANG X, WADL P A, POUNDERS C, et al.Evaluation of genetic diversity and pedigree within crapemyrtle cultivars using simple sequence repeat markers[J]. Journal of the American society for horticultural science, 2011,136(2):116-128.
[2] POOLER M R.‘Arapaho’ and ‘Cheyenne’ lagerstroemia[J]. HortScience, 2006,41(3):855-856.
[3] EGOLF D R. ‘Apalachee’,‘Comanche’,‘Lipan’,‘Osage’,‘Sioux’,and ‘Yuma’ Lagerstroemia[J]. HortScience, 1987,(22):674-677.
[4] 杨彦伶, 李振芳, 徐红梅, 等. 紫薇与福建紫薇种间杂交F₁代遗传性状分析[J]. 东北林业大学学报, 2018,46(9):31-34.
[5] 蔡明, 王晓玉, 张启翔, 等. 紫薇品种与尾叶紫薇种间杂交亲和性研究[J]. 西北植物学报, 2010(4):697-701.
[6] POUNDERS C,RINEHART T,SAKHANOKHO H.Evaluation of interspecific hybrids between Lagerstroemia indica and L. speciosa[J]. HortScience, 2007,42(6):1317-1322.
[7] 胡杏, 鞠易倩, 叶远俊, 等.紫薇×大花紫薇不同育性株系花粉生活力及柱头可授性对比分析[J]. 河南农业大学学报, 2014,48(2):145-149.
[8] 焦垚. 大花紫薇与紫薇杂交后代重要观赏性状遗传分析[D]. 北京:北京林业大学, 2017.
[9] 聂硕, 张林, 王峰, 等. 紫薇杂种F₁代的性状表现与倍性研究[J]. 山东农业科学, 2017(1):17-22.
[10] 王晓娇. 紫薇同源四倍体的获得及组培快繁技术研究[D]. 北京:北京林业大学, 2013.
[11] 杨冰洁, 陈晶鑫, 唐婉, 等. 紫薇属植物染色体制片技术优化[J]. 广东农业科学, 2012,39(17):135-137.
[12] 宋新红. 紫薇多倍体的诱导、鉴定及内多倍性[D].济南:山东农业大学, 2012.
[13] YE Y M, TONG J, SHI X P, et al.Morphological and cytological studies of diploid and colchicine-induced tetraploid lines of crape myrtle (Lagerstroemia indica L.)[J]. Scientia horticulturae, 2010,124(1):95-101.
[14] 宋平. 紫薇再生体系的建立及多倍体诱导研究[D]. 北京:北京林业大学, 2009.
[15] 童俊, 叶要妹, 冯彪, 等. 秋水仙素诱导三种紫薇多倍体的研究[J]. 园艺学报,2009,36(1):127-132.
[16] 陈瑞阳. 中国主要经济植物基因组染色体图谱:中国园林花卉植物染色体图谱[M]. 北京:科学出版社, 2003.
[17] ALI R.Chromosome numbers in some species of Lagerstroemia[J]. Current science, 1977,46:579-580.
[18] SINGHAL V K, GILL B S.SOCGI plant chromosome number reports - II[J]. Journal of cytology and genetics, 1984,19:115-117.
[19] GOLDBLATT P, PORTER P L II. The Index to Plant Chromosome Numbers (Ipcn): Three decades of publication by the missouri botanical garden come to an end[J]. Annals of the missouri botanical garden, 2011,98(2):226-227.
[20] MEHRA P N, BAWA K S.Chromosomal evolution in tropical hardwoods[J]. Evolution,1969,23(3):466-481.
[21] DARLINGTON C D, WYLIE A P.Chromosome atlas of flowering plants[M]. London, UK: George Allen and Unwin Ltd., 1956.
[22] BOWDEN W M.A list of chromosome numbers in higher plants I. Acanthaceae to Myrtaceae[J]. American journal of botany, 1945,32(2):81-92.
[23] GOLDBLATT P.Index to plant chromosome numbers 1975—1978[M]. St Louis: Missouri botanical garden, 1981.
[24] SONG P, WANG X, CAI M, et al.Research on identification of polyploids by flow cytometry in Lagerstroemia indica and Lagerstroemia subcostata[J]. Acta horticulturae, 2012,935:207-212.
[25] 陈磊. 紫薇无性系建立及多倍体诱导技术研究[D]. 天津:天津大学, 2011.
[26] ZHANG Q, LUO F, LIU L, et al.In vitro induction of tetraploids in crape myrtle (Lagerstroemia indica L.)[J]. Plant cell, tissue and organ culture (PCTOC), 2010,101(1):41-47.
[27] 刘亚琼. 中国紫薇属植物倍性研究及其cpDNA多样性分析[D]. 郑州:河南农业大学, 2010.
[28] 刘婷婷, 梁晓涵, 张烨, 等. 紫薇属及近缘属13种植物基因组大小测定[J]. 植物遗传资源学报, 2020,21(4):1020-1029.
[29] VRÁNA J,CÁPAL P,BEDNÁŘOVÁ M,et al. Flow cytometry in plant research: A success story[A]//NICK P,OPATRNY Z. Applied plant cell biology[M].Springer, 2014.395-430.
[30] DOLEŽEL J, GREILHUBER J, SUDA J. Estimation of nuclear DNA content in plants using flow cytometry[J]. Nature protocols, 2007,2(9):2233-2244.
[31] WEN G, DANG J, XIE Z, et al.Molecular karyotypes of loquat (Eriobotrya japonica) aneuploids can be detected by using SSR markers combined with quantitative PCR irrespective of heterozygosity[J]. Plant methods, 2020,16:22.
[32] 李伟强,戴晓港,李小平,等. 美洲黑杨种质材料倍性鉴定[J]. 南京林业大学学报(自然科学版), 2019,43(5):51-58.
[33] 解凯东, 王晓培, 王惠芹, 等. 以柑橘多胚性二倍体母本倍性杂交培育三倍体[J]. 园艺学报, 2014,41(4):613-620.
[34] 梁武军, 解凯东, 郭大勇, 等. 10个柑橘砧木类型同源四倍体的发掘与SSR鉴定[J]. 果树学报, 2014,31(1):1-6.
[35] 何敬峰, 李春峰, 丁美娟, 等. 四倍体野菊与露地菊‘紫秋裳’种间杂交 F₁代3个株系倍性鉴定[J]. 东北林业大学学报, 2019,47(5):1-3.
[36] PELLICER J, POWELL R F, LEITCH I J.The Application of flow cytometry for estimating genome size, ploidy level endopolyploidy, and reproductive modes in plants[J]. Methods in molecular biology, 2021,2222:325-361.
[37] 吕顺, 任毅, 王芳, 等. 利用流式细胞术快速鉴定169份香蕉种质资源的染色体倍性[J]. 果树学报, 2018,35(6):668-684.
[38] 彭婵, 李振芳, 马林江, 等. 紫薇属种间杂种SSR分子鉴定与遗传分析[J]. 北方园艺, 2020(13):83-90.
[39] 闫蕊, 阮成江, 吴波, 等. 油茶种质倍性的SSR-PAGE和SSRseq鉴定[J]. 分子植物育种, 2018,16(19):6390-6397.
[40] YAGI M, KIMURA T, YAMAMOTO T, et al.Estimation of ploidy levels and breeding backgrounds in pot carnation cultivars using flow cytometry and SSR markers[J]. Journal of the Japanese society for horticultural science, 2009,78(3):335-343.
[41] SCHIE S, CHAUDHARY R, DEBENER T.Analysis of a complex polyploid plant genome using molecular markers: Strong evidence for segmental allooctoploidy in garden dahlias[J]. The plant genome, 2014,7(3):1-6.
[42] AKOND M, JIN S, WANG X.Molecular characterization of selected wild species and miniature roses based on SSR markers[J]. Scientia horticulturae, 2012,147:89-97.
[43] 聂硕. 紫薇育种技术研究[D]. 山东泰安:山东农业大学,2016.
[44] 杨冰洁. 几种紫薇属植物染色体制片技术优化及原位杂交体系的建立[D]. 北京:北京林业大学, 2013.
[45] 李雯雯,刘立强,帕米尔·艾尼,等. 利用流式细胞术鉴定新疆野杏染色体倍性和DNA含量[J]. 农业生物技术学报,2019,27(3):542-550.
[46] 赖彪,吴传龙,秦永华,等. 流式细胞仪测定荔枝倍性和基因组大小的细胞核提取液筛选与应用[J]. 果树学报,2019,36(7):939-946.
[47] 田新民, 周香艳, 弓娜. 流式细胞术在植物学研究中的应用——检测植物核DNA含量和倍性水平[J]. 中国农学通报, 2011(9):21-27.
[48] 宋新红, 丰震, 谷衍川, 等. 紫薇多倍体的诱导、鉴定及内多倍性研究[J]. 山东林业科技, 2012,42(2):1-7.
[49] 贾会霞, 姬慧娟, 胡建军, 等. 杨树新品种的SSR指纹图谱构建和倍性检测[J]. 林业科学, 2015,51(2):69-79.
[50] 刘琰玮, 李美育, 朱景乐, 等. 枣树多倍体种质资源的发掘及其SSR鉴定[J]. 分子植物育种, 2018,16(10):3395-3400.

Analysis on the ploidy of hybrid seeds between Lagerstroemia indica and Lagerstroemia speciosa

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 5

Recommended Articles

Metrics

Comments

[1]	YANG Yan-ling, LI Zhen-fang, MA Lin-jiang, WANG Rui-wen, ZHANG Xin-ye, PENG Chan, HUANG Guo-wei, CHEN Hui-ling. Comprehensive evaluation of superior individuals of dwarf crape myrtle using hierarchy-gray correlation method [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(11): 76-80.
[2]	LI Ling-li, ZOU Shi-hui, YU Zhi-yong, WANG Bao-ning, YANG Li-jun, ZHOU Li. Evaluation on the growth potential of Ginkgo biloba L. and Lagerstroemia indica L. planted in road green space of Chongqing city [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(8): 111-115.
[3]	SHA Fei, YANG Hai-niu, LIU Fang, YE Yao-mei. Effects of pruning intensity and number of remaining buds on second flowering of Lagerstroemia indica [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(5): 106-109.
[4]	WANG Ye-she, CHEN Li-jun, YANG Xian-jun, DUAN Lin-dong. New cultivars of Lagerstroemia indica Lagerstroemia indica ‘Danzhao Zihuan’ and Lagerstroemia indica ‘Honghuan Danzi’ [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(2): 123-124.
[5]	WANG Ye-she, CHEN Li-jun, YANG Xian-jun, DUAN Lin-dong. New cultivars of Lagerstroemia Zhie Zhizhi Fen and Honghuan Fenjing [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(11): 91-92.