Comparison of quality differences of flavonoids in Sophora japonica L. flowers and Robinia pseudoacacia L. flowers

doi:10.14088/j.cnki.issn0439-8114.2022.07.020

Abstract

Abstract: Sophora japonica L. flowers and Robinia pseudoacacia L. flowers are closely related varieties, both of which have medicinal and edible value and are rich in flavonoids. The standard of Sophora japonica L. flowers in pharmacopoeia was used to explore the difference of flavonoids between the two, so as to facilitate the distribution and clinical distinction. With rutin as the control, TLC qualitative comparison, colorimetry determination of total flavonoids content and HPLC determination of rutin content were conducted for the two sophora flower. The difference between the chromatograms of Sophora japonica L. flower and Robinia pseudoacacia L. flower was very obvious. Rutin was the main chemical composition in Sophora japonica L. flower, and there were different principal components from rutin in Robinia pseudoacacia L. flower. The determination results showed that the contents of total flavonoids and rutin of Sophora japonica L. flowers were about 1.5 times and 5~10 times of that of Robinia pseudoacacia L. flowers, respectively. The total flavonoids content of the two kinds of Sophora japonica L. flowers was greater than 8%; the total flavonoids of the same species of Sophora japonica L. flowers from different origins showed differences; after processing, the contents of total flavonoids and rutin gradually decreased in the order of raw Sophora japonica L. flowers, fried Sophora japonica L. flowers and Sophora japonica L. flowers charcoal. The chromatographic differences between the two kinds of Flos Sophorae were obvious, which could be used for the qualitative identification of the two; the contents of total flavonoids and rutin in Robinia pseudoacacia L. flowers were significantly lower than those in Sophora japonica. L. flowers There were differences in the amount of the same species of Sophora japonica L.flowers from different habitats, and there was little difference in chromatogram. The flavonoids in Flos Sophorae after processing changed to some extent; the results can be used for the comparison of flavonoids differences between the two.

Key words: Sophora japonica L. flowers, Robinia pseudoacacia L. flowers, total flavonoids, thin layer chromatography, colorimetry, high performance liquid chromatography

CLC Number:

FU Feng-ping, GAO Xin-yue, YU Jia, WU Jian-ying, YU Ling, CHEN Hong-ying. Comparison of quality differences of flavonoids in Sophora japonica L. flowers and Robinia pseudoacacia L. flowers[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(7): 108-112.

References

[1] 陈晗雯,何富乐.辨一辨洋槐与国槐之花[J].健康博览,2018(8):32-33.
[2] 国家药典委员会.中华人民共和国药典[M]. 北京:中国医药科技出版社,2020.
[3] 吴虹霁,兰昌云,陈媛. 槐花的研究进展[J]. 广东微量元素科学, 2006, 13(6):1-6.
[4] 孙国禄,赵强,董晓宁,等. 槐花化学成分及药理作用研究[J]. 中兽医医药杂志, 2009, 28(6):24-27.
[5] 刘琳, 程伟. 槐花化学成分及现代药理研究新进展[J]. 中医药信息, 2019, 36(4):125-128.
[6] 王林,章敏,胡秋辉.刺槐花营养功能成分及其开发利用[J].食品科学, 2006,27(2):274-276.
[7] CVETKOVIĆ D M, JOVANKIĆ J V, MILUTINOVIĆ M G,et al.The anti-invasive activity of Robinia pseudoacacia L. and Amorpha fruticosa L. on breast cancer MDA-MB-231 cell line[J]. Biologia, 2019,74:915-928.
[8] 王颖, 荀守华, 张元帅,等. 6种刺槐花营养成分和活性物质检测与分析[J]. 山东林业科技, 2018,48(5):23-26,30.
[9] 宋永芳, 罗嘉梁,解荷锋. 刺槐花的化学成分研究[J]. 林产化学与工业, 1992(4):321-326.
[10] VEITCH N C, ELLIOTT P C, KITE G C, et al.Flavonoid glycosides of the black locust tree, Robinia pseudoacacia (Leguminosae)[J]. Phytochemistry, 2010, 71(4):479-486.
[11] 鄢长余. 刺槐花化学成分与指纹图谱研究[D]. 沈阳:辽宁中医药大学, 2010.
[12] 李艳艳, 初正云, 翟延君,等. 刺槐花总黄酮提取工艺研究[J]. 辽宁中医药大学学报,2011,13(5):87-88.
[13] 向昌国, 向宁, 黄成龙,等. 响应面法优化刺槐花黄酮类化合物的微波提取工艺[J]. 食品科学, 2011,32(22):32-36.
[14] 陈叶青. 凉血止血药炒炭时黄酮类成分的变化规律与止血作用的相关性研究[D]. 南京:南京中医药大学, 2016.
[15] 赵雍, 郭静, 刘婷,等. 槐花制炭后新止血成分的药理研究[J]. 中国中药杂志, 2010, 35(17):2346-2349.
[16] 李娆娆,原思通.中药槐花炮制历史沿革研究[J].中国中药杂志,2004(11):72-76.
[17] 朱洪剑, 李鑫. 不同炮制方法对槐花有效成分百分含量的影响[J]. 哈尔滨商业大学学报(自然科学版),2018,34(4):392-397,401.