Isolation and identification of microorganism in larvae of naturally dead Batocera lineolata

doi:10.14088/j.cnki.issn0439-8114.2022.07.013

Abstract

Abstract: In order to find biocontrol bacteria with insecticidal activity against Batocera lineolata, and develop efficient microbial agents for biological control of Batocera lineolata, the traditional method of microbial isolation and culture combined with 16S rDNA gene sequencing was used to isolate and identify the microorganisms in the larvae of Batocera lineolata. The results showed that 21 strains of bacteria were classified into 12 genera including Klebsiella, Serratia and Alcaligenes, respectively. The 8 strains of fungi are classified into 6 genera including Clonostachys, Fusariums and Cordyceps. The insecticidal activities and exploitation potential of Serratia, Cordyceps and Clonostachys were discussed.

Key words: Batocera lineolata, death larvae, internal microorganism, isolation and identification

CLC Number:

LI Bei-na, YANG Zhen-de, LIN Ye-bang, XU Lei, WEI Shan-fang, BAN Zhong-hua. Isolation and identification of microorganism in larvae of naturally dead Batocera lineolata[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(7): 69-76.

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