Abstract: The application effect of foliar spraying amino acid selenium fertilizer on selenium rich Summer Black grape was studied, and the characteristics of selenium enrichment and the distribution of selenium forms were investigated. The results showed that the best concentration of selenium solution was 250 mg/kg, and the effect was obvious; Spraying selenium fertilizer regularly from the growth and blooming stage of Summer Black grape could significantly improve the quality of leaves and help to enrich organic selenium, with the highest selenium content of 138 μg/kg. There was a positive correlation between the period of selenium enrichment and the effect of selenium enrichment （R² = 0.959 4）, the selenium enrichment effect was remarkable. The change and distribution of different forms of selenium （alkali soluble, acid soluble, Tris-HCl state, water-soluble） in selenium rich grapes were further investigated. The results showed that the extraction rate of alkaline selenium was the best at 40 ℃ and 80 min.

Key words: Summer Black grape, selenium enrichment, amino acid selenium, soluble fraction selenium