Community structure and spatial distribution characteristics of culturable bacteria in Dongting lake

doi:10.14088/j.cnki.issn0439-8114.2022.07.003

Abstract

Abstract: The physical and chemical characteristics of water and sediment samples in Dongting lake area were analyzed, and the bacterial community structure was investigated by culture-dependent method and 16S rRNA gene sequence analysis. The results show that the water body in Dongting lake is neutral to slightly alkaline, and the comprehensive trophic level index （TLI） of East, South and West Dongting lake are 43.77,46.26 and 48.16, respectively, all of which belong to mesotrophic state. A total of 94 bacterial strains are obtained from water and sediment samples of Dongting lake, belonging to 4 phyla,17 genera and 41 species. The dominant phylum is Proteobacteria, followed by Firmicutes, and the dominant genus is Acinetobacter. The bacterial community structure is quite different in spatial distribution. Among 17 genera obtained from water and sediment samples, only five genera are shared, eight genera are unique to water samples, and four genera are unique to sediment samples. In addition, the species richness and abundance of bacteria in water and sediment samples of East Dongting lake are higher than those in the other two lakes. The results further reveal that Dongting lake area is rich in bacteria resources, and the differences in spatial distribution of bacterial communities are comparatively obvious. The diversity of bacterial communities decreased with the increase of eutrophication level. All of the above indicates that the lake area has been polluted at different levels, which leads to the differences in physical and chemical indicators of the water quality, and then affects the structure and diversity of bacterial community. Therefore, changes in physical and chemical factors caused by the external pollution have a greater impact on the microbial community structure and diversity.

Key words: Dongting lake, 16S rRNA gene, bacterial community structure, comprehensive trophic level index

CLC Number:

X172

SUN Xia, LIU Yang, WANG Fang, ZHANG Hu, MAO Shu-min, QI Cui-ying. Community structure and spatial distribution characteristics of culturable bacteria in Dongting lake[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(7): 13-19.

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