Abstract: NADH dehydrogenase subunit 1 is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase （Complex I） that functions in the transfer of electrons from NADH to the respiratory chain. To obtain the information of ND1 gene in Pteromys volans, bioinformatics methods were used in this article to analyze ND1 in there areas： physicochemical properties of ND1, hydrophilic/hydrophobic properties, secondary structure, tertiary structure, transmembrane structure, protein modification sites and homology of ND1 in Pteromys volans. The results in this research indicated that 318 amino acids were found in ND1 gene, which was a stable hydrophilic protein. The main secondary structure of ND1 protein was α-helix, random coil and extended strand. The siberian flying squirrel （Pteromys volans） and the particolored flying squirrel （Hylopetes alboniger） were found to be more closely related by compaired the ND1 gene in the siberian flying squirrel with 18 other animal species.

Key words: Pteromys volans, NADH dehydrogenase subunit 1, respiratory chain, bioinformatics