Analysis of endophytic bacterial diversity and metabolic function in Pinellia ternata from Hubei based on 16S rDNA

doi:10.14088/j.cnki.issn0439-8114.2021.23.038

Abstract

Abstract: In order to explore the diversity, abundance and flora function of endophytic bacteria in Pinellia ternata in Hubei, this study randomly collected samples of Pinellia ternata plants, extracted endophytic bacterial genomic DNA, and obtained 16S rDNA V3~V4 fragments by PCR amplification. The IlluminaMiSeq high-throughput sequencing technology was used to analyze the endophytic bacterial flora structure in Pinellia ternata plants. A total of 39 537 effective sequences and 681 OTUs for species classification were obtained, including 6 phylum, 10 class, 23 order, 29 family, 45 genus, 26 species of bacteria. The analysis of colony diversity indicated that Proteobacteria was the dominant bacteria atthephylumlevel, Mitochondria and Pseudomonadaceae were the dominant bacteriaat the family level. Functional prediction results indicated that most of the genes encoded by endophytic bacteria were related to metabolism, followed by genetic information processing and environmental information processing.

Key words: Pinellia ternate, endophytic bacteria, diversity, flora function

CLC Number:

S482.2⁺92

WANG Fan-fan, TANG Tao, GUO Jie, DUAN Yuan-yuan, GUO Xiao-liang, YOU Jing-mao. Analysis of endophytic bacterial diversity and metabolic function in Pinellia ternata from Hubei based on 16S rDNA[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(23): 172-176.

References 18

[1]	黄凤英, 高健美, 龚其海. 半夏药理作用及其毒性研究进展[J]. 天然产物研究与开发, 2020(10):1773-1781.
[2]	高振杰, 罗沙, 周建雄, 等. 半夏的研究进展[J]. 四川中医, 2019(4):212-215.
[3]	钱广涛,薛涛,张爱民,等.半夏无公害栽培技术体系探讨[J].世界中医药,2018,13(12):2949-2955.
[4]	AFZAL M,KHAN QM, SESSITSCH A.Endophytic bacteria:Prospects and applications for the phytoremediation of organic pollutants[J].Chemosphere,2014,117(1):232-242.
[5]	阳湖荣,陈飘雪,戴传超.白术内生细菌分离与促生活性[J].生态学杂志,2019,38(12):3680-3688.
[6]	冯维维,武美贤,司雨婷,等.中华补血草内生与根际具ACC脱氨酶活性细菌的筛选及其生物多样性[J].微生物学报,2016,56(4):719-728.
[7]	YAISH M W, ANTONY I, GLICK B R.Isolation and characterization of endophytic plant growth-promoting bacteria from date palm tree (Phoenix dactylifera L.) and their potential role in salinity tolerance[J]. Antonie van leeuwenhoek,2015,107(6):1519-1532.
[8]	吕艳娜, 孙小萌, 赵芮, 等. 药用植物内生细菌多样性及其生物学作用[J]. 中国野生植物资源,2017(6):45-49.
[9]	ASNICARF,WEINGART G, TICKLE T L, et al.Compact graphical representation of phylogenetic data and metadata with GraPhlAn[J]. Peer J, 2015,3:e1029.
[10]	LANGILLE M G I, ZANEVELD J, CAPORASO J G, et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences[J]. Nature biotechnology,2013,31(9):814-821.
[11]	赵佳琛,王艺涵,金艳,等.经典名方中半夏与天南星的本草考证[J].中国现代中药,2020,22(8):1361-1380.
[12]	张洁, 万芬, 李玉权,等. 半夏内生细菌的分离与鉴定[J]. 贵州农业科学,2014(3):94-97.
[13]	张盼盼. 邳半夏内生细菌多样性及其对邳半夏次生代谢产物积累的影响[D].江苏徐州:江苏师范大学,2017.
[14]	刘建玲, 陈宝宝, 刘永红, 等. 半夏内生菌的分离与初步鉴定[J]. 中国中药杂志,2009(18):2305-2307.
[15]	周佳宇, 贾永, 王宏伟, 等. 茅苍术叶片可培养内生细菌多样性及其促生潜力[J]. 生态学报,2013(4):1106-1117.
[16]	QIN S, ZHANG Y J, YUAN B, et al.Isolation of ACC deaminase-producing habitat-adapted symbiotic bacteria associated with halophyteLimoniumsinense(Girard) Kuntze and evaluating their plant growth-promoting activity under salt stress[J]. Plant and soil, 2014, 374(1-2):753-766.
[17]	苏昊. 半夏内生真菌的多样性及其药化研究[D].贵阳:贵州大学,2009.
[18]	刘建玲, 陈宝宝, 雷毅, 等. 半夏产生物碱内生菌的分离及其抑菌活性的初步研究[J]. 西北植物学报,2010(4):832-837.