Expression analysis of PpPRR5 gene in pear genotypes with different degrees of photosynthetic rate

doi:10.14088/j.cnki.issn0439-8114.2021.23.037

Abstract

Abstract: Improvement in photosynthesis is an effective way to further increase yield potential in fruit crops. Circadian clock genes could be adjusted by environmental cues, and thereby regulate photosynthesis. The differences of photosynthesis of different types of pear varieties （including Pyrus pyrifolia, Pyrus bretschneider and western pear） were studied and analyzed, and the expression level and change pattern of PpPRR5 gene in different varieties were detected. The results showed that significantly different levels of net photosynthetic rate were observed in five pear genotypes. Pyrus pyrifolia Nakaicv. ‘Cuiguan' had the highest levels of net photosynthetic rate. Pyrus bretschneider maintained moderate levels of net photosynthetic rate. Pyrus communis L. cv. ‘Abate Fetel' showed the lowest levels of net photosynthetic rate. There was a negative correlation between the expression of circadian clock gene PpPRR5 and the capacity of pear genotype for photosynthetic rate. The result suggests that PpPRR5 acts as a negative regulator of photosynthetic performance.

Key words: pear, photosynthesis, different genotypes, PpPRR5, expression levels

CLC Number:

Q819
S661.2

LIU Zheng, AN Lin, WU Tao, QIN Zhong-qi, YANG Li, CHENG Yin-sheng. Expression analysis of PpPRR5 gene in pear genotypes with different degrees of photosynthetic rate[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(23): 168-171.

References 23

[1]	INOUE K, ARAKI T, ENDO M.Oscillator networks with tissue-specific circadian clocks in plants[J]. Sem Cell Dev Biol, 2018, 83:78-85.
[2]	CANO-RAMIREZ D, DODD A N.New connections between circadian rhythms, photosynthesis, and environmental adaptation[J]. Plant cell environ, 2018, 41:2515-2517.
[3]	NOHALES M A,KAY S A.Molecular mechanisms at the core of the plant circadian oscillator[J]. Nat Struct Mol Biol,2016,23:1061-1069.
[4]	ZHANG Y,PFEIFFER A,TEPPERMAN J M,et al. Central clock components modulate plant shade avoidance by directly repressing transcriptional activation activity of PIF proteins[J]. Proc natlacadsci USA,2020,117:3261-3269.https://doi.org/10.1073/pnas.1918317 117.
[5]	HENRIQUES R, RAPDI C, AHMAD Z, et al.Circadian regulation of plant growth[J]. Annuplant reviews, 2018, 1:1-29.
[6]	SRIVASTAVA D, SHAMIM M, KUMAR M, et al.Role of circadian rhythm in plant system: An update from development to stress response[J]. Environ Exp Bot, 2019, 162:256-271.
[7]	RESCO DE DIOS V, LOIK M E, et al. Genetic variation in circadian regulation of nocturnal stomatal conductance enhances carbon assimilation and growth[J]. Plant cell environ, 2016, 39:3-11.
[8]	GARICA-PLAZAOLA J I,FERNANDEZ-MARIN B,FERRIO J P, et al. Endogenous circadian rhythms in pigment composition induce changes in photochemical efficiency in plant canopies[J]. Plant cell environ, 2017, 40:1153-1162.
[9]	HAYDON M J, MIELCZAREK O, ROBERTSON F C, et al.Photosynthetic entrainment of the Arabidopsis thaliana circadian clock[J]. Nature,2013,502:689-692.
[10]	KO D K,ROHOZINSKI D,SONG Q,et al.Temporal shift of circadian-mediated gene expression and carbon fixation contributes to biomass heterosis in maize hybrids[J]. Plosgenet,2016,12:e1006197.
[11]	NAKAMICHI N, KIBA T, KAMIOKA M, et al.Transcriptional repressor PRR5 directly regulates clock-output pathways[J]. Proc natlacadsci USA, 2012, 109:17123-17128.
[12]	TODA Y, KUDO T, KINOSHITA T, et al.Evolutionary insight into the clock-associated PRR5 transcriptional network of flowering plants[J]. Sci Rep, 2019, 9:2983.
[13]	NAKAMICHI N, KIBA T, HENRIQUES R, et al.PSEUDO-RESPONSE REGULATORS 9,7, and 5 are transcriptional repressors in the Arabidopsis Circadian Clock[J]. Plant cell,2010,22:594-605.
[14]	SATO E, NAKAMICHI N, YAMASHINO T, et al.Aberrant expression of the Arabidopsis circadian-regulated APRR5 gene belonging to the APRR1/TOC1 quintet results in early flowering and hypersensitiveness to light in early photomorphogenesis[J]. Plant cell physiol, 2002, 43:1374-1385.
[15]	YAMAMOTO Y, SATO E, SHIMIZU T, et al.Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet implicated in circadian rhythm, control of flowering time,and early photomorphogenesis[J]. Plant cell physiol,2003,44:1119-1130.
[16]	NAKAMICHI N, KITA M, ITO S, et al.PSEUDO-RESPONSE REGULATORS, PRR9, PRR7 and PRR5, together play essential roles close to the circadian clock of Arabidopsis thaliana[J]. Plant cell physiol, 2005, 46:686-698.
[17]	RUTS T,MATSUBARA S, WIESE-KLINKENBERG A, et al.Aberrant temporal growth pattern and morphology of root and shoot caused by a defective circadian clock in Arabidopsis thaliana[J]. Plant J, 2012, 72:154-161.
[18]	MATSUSHIKA A,MAKINO S,KOJIMA M, et al.Circadian waves of expression of the APRR1/TOC1 family of Pseudo-Response Regulators in Arabidopsis thaliana: Insight into the plant circadian clock[J]. Plant cell physiol, 2000, 41:1002-1012.
[19]	NOHALES MA, KAY SA.Molecular mechanisms at the core of the plant circadian oscillator[J]. Nat Struct Mol Biol,2016,23:1061-1069.
[20]	XIE Q, WANG P, LIU X, et al.LNK1 and LNK2 are transcriptional coactivators in the Arabidopsis circadian oscillator[J]. Plant cell, 2014, 26:2843-2857.
[21]	KIBA T, HENRIQUES R, SAKAKIBARA H, et al.Targeted degradation of PSEUDO-RESPONSE REGULATOR5 by an SCFZTL complex regulates clock function and photomorphogenesis in Arabidopsis thaliana[J]. Plant cell, 2007, 19:2516-253.
[22]	LIU Z,AN L,LIN S, et al.Comparative physiological and transcriptomic analysis of pear leaves under distinct training systems[J]. Sci Rep, 2020, 10:18892.
[23]	LIU Z, CHENG K, QIN Z, et al.Selection and validation of suitable reference genes for qRT-PCR analysis in pear leaf tissues under distinct training systems[J].PLos one,2018,13(8):e0202472.