Rapid screening and identification of a Lysinibacillus pakistanensis strain producing indole-3-aceticacid

doi:10.14088/j.cnki.issn0439-8114.2021.23.014

Abstract

Abstract: 1920 Bacillus strains were cultivated in selective media in 96-well deep well plate and rapidly screenedthrough Salkowski colorimetric method. 12 strains producing indole-3-acetic acid （IAA） were obtained， which had obvious chromatic reaction. The 12 strains were rescreened and quantitively measured. One strain could produce IAA of 118.23±5.55 mg/L which was selected. After gene sequencing and sequence alignment of 16S rDNA， the strain was identified as Lysinibacillus pakistanensis.

Key words: IAA, Screening, Lysinibacillus pakistanensis

CLC Number:

S182

MIN Yong, LIU Xiao-yan, CHEN Ling, ZHU Lei, QIU Yi-min, ZHOU Rong-hua. Rapid screening and identification of a Lysinibacillus pakistanensis strain producing indole-3-aceticacid[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(23): 68-70.

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Rapid screening and identification of a Lysinibacillus pakistanensis strain producing indole-3-aceticacid

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