Identification on heavy metal-resistant Enterobacter ludwigii GL-2 and effects on growth of wheat

doi:10.14088/j.cnki.issn0439-8114.2021.23.010

Abstract

Abstract: In order to isolate the plant growth-promoting rhizobacteria（PGPR）that exhibit heavy metal resistance, the LB medium supplemented with Cd and the siderophores medium were used to screen suitable strains. Minimal inhibitory concentrations （MICs） of GL-2 strain was determined by plate dilution method. The passivation effects to Pb and Cd on the growth o f wheat under GL-2 strain were added to MS medium in the different treatments. The promoting effect of GL-2 was studied on the growth of wheat by pot experiments. The results showed that the strain GL-2 belongs to Enterobacter ludwigii based on molecular biology and physiological and biochemical identification. GL-2 had a strong tolerance to heavy metals, and the minimum inhibitory concentration of Pb was 650 mg/L.The MS medium added to GL-2 strains could relieve heavy metal influence on wheat growth effectively. There was a significantly effect to increase the root fresh weight, tiller numbers and root dry weight of wheat within GL-2 strains.

Key words: Enterobacter ludwigii, wheat, heavy metal, plant growth-promoting

CLC Number:

S512.1

CAI Gao-lei, ZHOU Yang, ZHAO Chang-song, PENG Xuan-he, ZHANG zhen, ZHAO Jian-ning. Identification on heavy metal-resistant Enterobacter ludwigii GL-2 and effects on growth of wheat[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(23): 44-48.

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