Gene editing technology and its application prospects in mulberry breeding

doi:10.14088/j.cnki.issn0439-8114.2021.23.002

Abstract

Abstract: The mechanism of three gene editing techniques and the application of CRISPR/Cas9 gene editing technology in plant breeding were reviewed. According to the current situation of mulberry breeding, the application prospect of CRISPR/cas9 gene editing technology in mulberry functional genomics and genetic improvement was prospected, in order to provide reference and new ideas for mulberry diversified germplasm innovation.

Key words: mulberry(Morus alba L.), gene editing, CRISPR/Cas, genetic improvement

CLC Number:

HUANG Jin, LI Yong, YU Cui, ZHU Zhi-xian, MO Rong-li, DONG Zhao-xia, HU Xing-ming, DENG Wen. Gene editing technology and its application prospects in mulberry breeding[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(23): 8-14.

References 82

[1]	买买提依明,夏庆友,吴丽莉,等.新疆沙漠桑树的研究现状与发展方向[J].北方蚕业,2007(2):1-4.
[2]	周建华. 湖北省蚕桑产业发展回顾与展望[J].湖北农业科学,2017,56(15):2880-2882,2887.
[3]	侯韶敏. 桑树经济价值及播种育苗技术[J].山西林业,2021(2):32-33.
[4]	AGARWAL S, KANWAR K.Comparison of genetic transformation in Morus alba L. via different regeneration systems[J]. Plant cell reports,2007,26(2):177-85.
[5]	郑红艳,王磊.CRISPR/Cas基因编辑技术及其在作物育种中的应用[J].生物技术进展,2018,8(3):185-190.
[6]	左鑫,李欣容,李铭铭,等.基因编辑技术及其在药用植物中的应用展望[J].中国现代中药,2020,22(12):2108-2114,2121.
[7]	KIM Y G, CHA J, CHANDRASEGARAN S.Hybrid restriction enzymes:Zinc finger fusions to Fok I cleavage domain[J]. Proceedings of the national academy of sciences of the United States of America,1996,93(3):1156-1160.
[8]	CHRISTIAN M, CERMAK T, DOYLE E L, et al.Targeting DNA double-strand breaks with TAL effector nucleases[J]. Genetics,2010,186(2):757-761.
[9]	JINEK M, CHYLINSKI K, FONFARA I, et al.A programmable Dual-RNA-Guided DNA endonuclease in adaptive bacterial immunity[J]. Science,2012,337(6096):816-821.
[10]	ZHANG Y, MASSEL K, GODWIN ID, et al.Applications and potential of genome editing in crop improvement[J]. Genome biology,2018,19(1):210.
[11]	DOUDNA J A, CHARPENTIER E.The new frontier of genome engineering with CRISPR-Cas9[J]. Science,2014,346(6213):1258096.
[12]	URNOV F D, REBAR E J, HOLMES M C, et al.Genome editing with engineered Zinc finger nucleases[J]. Nature reviews genetics,2010,11(8):636-46.
[13]	CHUGUNOVA A A, DONTSOVA O A, SERGIEV P V.Methods of genome engineering: A new era of molecular biology[J]. Biochemistry (Moscow),2016,81(7):662-677.
[14]	MANI M, KANDAVELOU K, DY F J, et al.Design, engineering, and characterization of Zinc finger nucleases[J]. Biochemical and biophysical research communications,2005,335(2):447-57.
[15]	CHEN K, WANG Y, ZHANG R,et al.CRISPR/Cas genome editing and precision plant breeding in agriculture[J]. Annual review of plant biology,2019,70:667-697.
[16]	CARROLL D.Progress and prospects: Zinc-finger nucleases as gene therapy agents[J]. Gene therapy,2008,15(22):1463-1468.
[17]	ALEXANDER W G.A history of genome editing in Saccharomyces cerevisiae[J]. Yeast,2018,35(5):355-360.
[18]	BOGDANOVE A J, SCHORNACK S, LAHAYE T.TAL effectors:Finding plant genes for disease and defense[J]. Current opinion in plant biology,2010,13(4):394-401.
[19]	MOSCOU M J, BOGDANOVE A J.A simple cipher governs DNA recognition by TAL effectors[J]. Science,2009,326(5959):1501.
[20]	CONG L,RAN F A,COX D,et al.Multiplex genome engineering using CRISPR/Cas systems[J]. Science,2013,339(6121):819-823.
[21]	MAKAROVA K S,HAFT D H,BARRANGOU R,et al.Evolution and classification of the CRISPR-Cas systems[J]. Nature reviews microbiology,2011,9(6):467-477.
[22]	MAKAROVA K S, KOONIN E V.Annotation and classification of CRISPR-Cas systems[J]. Methods in molecular biology (Clifton, N.J.),2015,1311:47-75.
[23]	KOONIN E V, MAKAROVA K S, ZHANG F.Diversity, classification and evolution of CRISPR-Cas systems[J]. Current opinion in microbiology,2017,37:67-78.
[24]	NISHIMASU H,RAN F A,HSU P D,et al.Crystal structure of Cas9 in complex with guide RNA and target DNA[J]. Cell,2014,156(5):935-949.
[25]	GASIUNAS G,BARRANGOU R,HORVATH P,et al.Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria[J]. Proceedings of the national academy of sciences of the United States of America,2012,109(39):E2579-86.
[26]	MARRAFFINI L A, SONTHEIMER E J.Self versus non-self discrimination during CRISPR RNA-directed immunity[J]. Nature,2010,463(7280):568-571.
[27]	BARRANGOU R,FREMAUX C,DEVEAU H,et al.CRISPR provides acquired resistance against viruses in prokaryotes[J]. Science,2007,315:1709-1712.
[28]	WIEDENHEFT B, STERNBERG S H, DOUDNA JA.RNA-guided genetic silencing systems in bacteria and archaea[J]. Nature, 2012,482(7385):331-338.
[29]	STERNBERG S, HREDDING S, JINEK M, et al.DNA interrogation by the CRISPR RNA-guided endonuclease Cas9[J]. Biophysical journal,2014,106(2):695a.
[30]	包爱科,白天惠,赵天璇,等.CRISPR/Cas9系统:基因组定点编辑技术及其在植物基因功能研究中的应用[J].草业学报,2017,26(7):190-200.
[31]	刘耀光,李构思,张雅玲,等.CRISPR/Cas植物基因组编辑技术研究进展[J].华南农业大学学报,2019,40(5):38-49.
[32]	ZETSCHE B, GOOTENBERG J S, ABUDAYYEH O O, et al.Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system[J]. Cell, 2015, 163(3):759-771.
[33]	MAHFOUZ M M.Genome editing: The efficient tool CRISPR-Cpf1[J]. Nat plants, 2017, 3:17028.
[34]	SHMAKOV S, ABUDAYYEH O O, MAKAROVA K S, et al.Discovery and functional characterization of diverse class 2 CRISPR-Cas systems[J]. Molecular cell,2015,60(3):385-397.
[35]	陈赢男,陆静.CRISPR/Cas9系统在林木基因编辑中的应用[J].遗传,2020,42(7):657-668.
[36]	SHAN Q W, WANG Y P, LI J, et al.Targeted genome modification of crop plants using a CRISPR-Cas system[J]. Nature biotechnology,2013,31(8):686-688.
[37]	XU R, YANG Y, QIN R, et al.Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice[J]. Journal of genetics and genomics,2016,43(8):529-532.
[38]	ZHOU J P, XIN X H, HE Y, et al.Multiplex QTL editing of grain-related genes improves yield in elite rice varieties[J]. Plant cell reports,2019,38(4):475-485.
[39]	MA X, FENG F, ZHANG Y, et al.A novel rice grain size gene OsSNB was identified by genome-wide association study in natural population[J]. PLoS genetics,2019,15(5): e1008191.
[40]	SUN Y, JIAO G, LIU Z, et al.Generation of high-amylose rice through CRISPR/Cas9-mediated targeted mutagenesis of starch branching enzymes[J]. Frontiers in plant science,2017,8:298.
[41]	杨平,陈春莲,姚晓云,等.利用基因编辑技术改良水稻直链淀粉含量与香味[J].分子植物育种,2020,18(3):915-923.
[42]	王加峰,郑才敏,刘维,等.基于CRISPR/Cas9技术的水稻千粒重基因tgw6突变体的创建[J].作物学报,2016,42(8):1160-1167.
[43]	徐鹏,王宏,涂燃冉等.利用CRISPR/Cas9系统定向改良水稻稻瘟病抗性[J].中国水稻科学,2019,33(4):313-322.
[44]	SHI J, GAO H, WANG H, et al. ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions[J]. Plant biotechnology journal,2017,15(2):207-216.
[45]	LI J, ZHANG H, SI X, et al.Generation of thermosensitive male- sterile maize by targeted knockout of the ZmTMS5 gene[J]. Journal of genetics and genomics,2017,44(9):465-468.
[46]	SVITASHEV S, YOUNG J, SCHWARTZ C, et al.Targeted mutagenesis, precise gene editing, and site- specific gene insertion in maize using Cas9 and guide RNA[J]. Plant physiology,2015,169:931-945.
[47]	ZENG R, LI Z, SHI Y, et al.Natural variation in a type-A response regulator confers maize chilling tolerance[J]. Nature communications,2021,12(1):4713.
[48]	WANG Y, CHENG X, SHAN Q, et al.Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew[J]. Nature biotechnology,2014,32(9):947-951.
[49]	WANG W, PAN Q, HE F, et al.Transgenerational CRISPRCas9 activity facilitates multiplex gene editing in allopolyploid wheat[J]. The CRISPR journal,2018,1(1):65-74.
[50]	DO P T, NGUYEN C X, BUI H T, et al.Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2-1A and GmFAD2-1B genes to yield a high oleic, low linoleic and alpha-linolenic acid phenotype in soybean[J]. BMC Plant Biology, 2019,19(1):311.
[51]	WANG J, KUANG H, ZHANG Z, et al.Generation of seed lipoxygenase-free soybean using CRISPR-Cas9[J]. The crop journal,2020,8(3):432-439.
[52]	LI M, CHEN R, JIANG Q Y, et al.GmNAC06, a NAC domain transcription factor enhances salt stress tolerance in soybean[J]. Plant molecular biology,2020,105(3):333-345.
[53]	NADAKUDUTI S S, STARKER C G, VOYTAS D F, et al.Genome editing in potato with CRISPR/Cas9[J]. Methods in molecular biology (Clifton, N.J.),2019,1917:183-201.
[54]	ZENG Z, HAN N, LIU C, et al.Functional dissection of HGGT and HPT in barley vitamin E biosynthesis via CRISPR/Cas9-enabled genome editing[J]. Annals of botany,2020,126(5):929-942.
[55]	GAO W, LONG L, TIAN X, et al.Genome editing in cotton with the CRISPR/Cas9 system[J]. Frontiers in plant science,2017,8:1364.
[56]	FAN D, LIU T T, LI C F, et al.Efficient CRISPR/Cas9-mediated targeted mutagenesis in populus in the first generation[J]. Scientific reports,2015,5:12217.
[57]	ZHOU X H,JACOBS T B,XUE L J,et al.Exploiting SNPs for biallelic CRISPR mutations in the outcrossing woody perennial Populus reveals 4-coumarate:CoA ligase specificity and redundancy[J].New phytologist,2015,208(2):298-301.
[58]	MUHR M, PAULAT M, AWWANAH M, et al.CRISPR/Cas9-mediated knockout of Populus BRANCHED1 and BRANCHED2 orthologs reveals a major function in bud outgrowth control[J]. Tree physiology,2018,38(10):1588-1597.
[59]	WAN S Z, LI C F, MA X D, et al.PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar[J]. Plant cell reports,2017,36(8):1263-1276.
[60]	CHARRIER A, VERGNE E, DOUSSET N, et al.Efficient targeted mutagenesis in apple and first time edition of pear using the CRISPR-Cas9 system[J]. Frontiers in plant science,2019,10:40.
[61]	VARKONYI-GASIC E, WANG T C, VOOGD C, et al.Mutagenesis of kiwifruit CENTRORADIALIS-like genes transforms a climbing woody perennial with long juvenility and axillary flowering into a compact plant with rapid terminal flowering[J]. Plant biotechnology journal,2019,17(5):869-880.
[62]	REN C, LIU X J, ZHANG Z, et al.CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)[J]. Scientific reports,2016,6:32289.
[63]	PENG A H, CHEN S C, LEI T G, et al.Engineering canker‐resistant plants through CRISPR/Cas9‐targeted editing of the susceptibility gene CsLOB1 promoter in citrus[J]. Plant biotechnology journal,2017,15(12):1509-1519.
[64]	GOMEZ M A, LIN Z D, MOLL T, et al.Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence[J]. Plant biotechnology journal,2019,17(2):421-434.
[65]	KIM H R, PATEL K R, THORPE T A.Regeneration of mulberry plants through tissue culture[J]. Botanical gazette,1985,146:335-340.
[66]	MACHII M.Leaf disc transformation of mulberry plant (Morus alba L.) by Agrobacterium Ti plasmid[J].Journal of insect biotechnology & sericology,1990,59:105-110.
[67]	MACHII M, SUNG G B, YAMANUCHI H, et al.Transient expression of GUS gene introduced into mulberry plant by particle bombardment[J]. Journal of insect biotechnology & sericology,1996,65:503-506.
[68]	KAPUR A, BHATNAGAR S, KHURANA P.Efficient regeneration from mature leaf explants of Indian mulberry via organogenesis[J]. Sericologia,2001,41:207-214.
[69]	SUGIMURA Y, MIYAZAKI J, YONEBAYASHI K, et al.Gene transfer by electroporation into protoplasts isolated from mulberry call[J]. Journal of insect biotechnology & sericology,1999,68:49-53.
[70]	OKA S, TEWARY P K.Induction of hairy roots from hypocotyls of mulberry (Morus indica L.) by Japanese wild strains of Agrobacterium rhizogenes[J]. Journal of insect biotechnology & sericology,2000,69:13-19.
[71]	BHATNAGAR S, KAPUR A, KHURANA P.Evaluation of parameters for high efficiency gene transfer via particle bombardment in Indian mulberry[J]. Indian journal of experimental biology,2002,40(12):1387-1392.
[72]	BHATNAGAR S, KHURANA P.Agrobacterium tumefaciens-mediated transformation of Indian mulberry, Morus indica cv. K-2:a time-phased screening strategy[J]. Plant cell reports,2003,21(7):669-675.
[73]	SARKAR T, MOGILI T, SIVAPRASAD V.Improvement of abiotic stress adaptive traits in mulberry (Morus spp.):An update on biotechnological interventions[J]. 3 Biotech,2017,7(3):214.
[74]	谈建中,楼程富,钟名其,等.大豆球蛋白基因表达载体的构建及对桑树的遗传转化[J].蚕业科学,1999,25(1):5-10.
[75]	管志文,谭智达,陈爱玉,等.农杆菌携带柞蚕抗菌肽基因转入桑树的研究[J].蚕业科学,1994,20(1):1-6.
[76]	王勇,贾仕荣,陈爱玉,等.抗菌肽基因导入桑树获得抗病转基因植株[J].蚕业科学,1998,24(3):136-140.
[77]	LAL S, GULYANI V, KHURANA P.Overexpression of hva1 gene from barley generates tolerance to salinity and water stress in transgenic mulberry (Morus indica)[J]. Transgenic research,2008,17:651-663.
[78]	CHECKER V G, CHIBBAR A K, KHURANA P.Stress-inducible expression of barley hva1 gene in transgenic mulberry displays enhanced tolerance against drought, salinity and cold stress[J].Transgenic research,2012,21(5):939-957.
[79]	DAS M, CHAUHAN H, CHHIBBAR A, et al.High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K-2, by constitutive and inducible expression of tobacco Osmotin[J]. Transgenic research,2011,20(2):231-246.
[80]	SAJEEVAN R S, NATARAJA K N, SHIVASHANKARA K S, et al.Expression of Arabidopsis SHN1 in Indian mulberry (Morus indica L.) increases leaf surface wax content and reduces post-harvest water loss[J]. Frontiers in plant science,2017,8:418.
[81]	SAEED B, DAS M, HAQ Q M R, et al. Overexpression of beta carotene hydroxylase-1 (bch1) in mulberry, Morus indica cv. K-2, confers tolerance against high-temperature and high-irradiance stress induced damage[J]. Plant cell tissue and organ culture,2015,120(3):1003-1015.
[82]	陈笑,冯献忠.基因组编辑技术在大豆遗传改良中的应用[J].农业生物技术学报,2021,29(4):789-798.